LOCUS       sequence-171520-        9027 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses sgRNA targeting human KRAS around codon G12.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9027)
  AUTHORS   Guernet A, Mungamuri SK, Cartier D, Sachidanandam R, Jayaprakash A, 
            Adriouch S, Vezain M, Charbonnier F, Rohkin G, Coutant S, Yao S, 
            Ainani H, Alexandre D, Tournier I, Boyer O, Aaronson SA, Anouar Y, 
            Grumolato L
  TITLE     CRISPR-Barcoding for Intratumor Genetic Heterogeneity Modeling and 
            Functional Analysis of Oncogenic Driver Mutations.
  JOURNAL   Mol Cell. 2016 Aug 4;63(3):526-38. doi: 
            10.1016/j.molcel.2016.06.017. Epub 2016 Jul 21.
  PUBMED    27453044
REFERENCE   2  (bases 1 to 9027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.06.017"; journalName: "Mol Cell"; date: 
            "2016-08-4- 4"; volume: "63"; issue: "3"; pages: "526-38"
COMMENT     Sequence Label: sgKRAS(A)
FEATURES             Location/Qualifiers
     source          1..9027
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          31..258
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      270..279
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             279..344
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             351..371
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             396..4496
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             396..4496
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4497..4544
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4560..4613
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4614..5213
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4614..4633)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     complement(5241..5258)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5247..5454
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5463..5603
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5463..5592
                     /label=AAV2 ITR
     rep_origin      5678..6133
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5765..5784)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5975..5996
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6150..6169)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6269..6291
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6329..6347)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6415..6519
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6520..6588,6589..7380)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6738..6757)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7551..8139
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8040..8059
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        8201..8449
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     8201..8221
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8372..8391
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8470..8545
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        8642..8927
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg cgcgctgcct tcgccccgtg
       61 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca
      121 caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagctgag caagaggtaa
      181 gggtttaagg gatggttggt tggtggggta ttaatgttta attacctgga gcacctgcct
      241 gaaatcactt tttttcaggt tggaccggtg ccaccatgga ctataaggac cacgacggag
      301 actacaagga tcatgatatt gattacaaag acgatgacga taagatggcc ccaaagaaga
      361 agcggaaggt cggtatccac ggagtcccag cagccgacaa gaagtacagc atcggcctgg
      421 acatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag gtgcccagca
      481 agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac ctgatcggag
      541 ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga accgccagaa
      601 gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc agcaacgaga
      661 tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg gtggaagagg
      721 ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg
      781 agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc gacaaggccg
      841 acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc cacttcctga
      901 tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc cagctggtgc
      961 agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg
     1021 ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc gcccagctgc
     1081 ccggcgagaa gaagaatggc ctgttcggaa acctgattgc cctgagcctg ggcctgaccc
     1141 ccaacttcaa gagcaacttc gacctggccg aggatgccaa actgcagctg agcaaggaca
     1201 cctacgacga cgacctggac aacctgctgg cccagatcgg cgaccagtac gccgacctgt
     1261 ttctggccgc caagaacctg tccgacgcca tcctgctgag cgacatcctg agagtgaaca
     1321 ccgagatcac caaggccccc ctgagcgcct ctatgatcaa gagatacgac gagcaccacc
     1381 aggacctgac cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag tacaaagaga
     1441 ttttcttcga ccagagcaag aacggctacg ccggctacat tgacggcgga gccagccagg
     1501 aagagttcta caagttcatc aagcccatcc tggaaaagat ggacggcacc gaggaactgc
     1561 tcgtgaagct gaacagagag gacctgctgc ggaagcagcg gaccttcgac aacggcagca
     1621 tcccccacca gatccacctg ggagagctgc acgccattct gcggcggcag gaagattttt
     1681 acccattcct gaaggacaac cgggaaaaga tcgagaagat cctgaccttc cgcatcccct
     1741 actacgtggg ccctctggcc aggggaaaca gcagattcgc ctggatgacc agaaagagcg
     1801 aggaaaccat caccccctgg aacttcgagg aagtggtgga caagggcgct tccgcccaga
     1861 gcttcatcga gcggatgacc aacttcgata agaacctgcc caacgagaag gtgctgccca
     1921 agcacagcct gctgtacgag tacttcaccg tgtataacga gctgaccaaa gtgaaatacg
     1981 tgaccgaggg aatgagaaag cccgccttcc tgagcggcga gcagaaaaag gccatcgtgg
     2041 acctgctgtt caagaccaac cggaaagtga ccgtgaagca gctgaaagag gactacttca
     2101 agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct
     2161 ccctgggcac ataccacgat ctgctgaaaa ttatcaagga caaggacttc ctggacaatg
     2221 aggaaaacga ggacattctg gaagatatcg tgctgaccct gacactgttt gaggacagag
     2281 agatgatcga ggaacggctg aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc
     2341 agctgaagcg gcggagatac accggctggg gcaggctgag ccggaagctg atcaacggca
     2401 tccgggacaa gcagtccggc aagacaatcc tggatttcct gaagtccgac ggcttcgcca
     2461 acagaaactt catgcagctg atccacgacg acagcctgac ctttaaagag gacatccaga
     2521 aagcccaggt gtccggccag ggcgatagcc tgcacgagca cattgccaat ctggccggca
     2581 gccccgccat taagaagggc atcctgcaga cagtgaaggt ggtggacgag ctcgtgaaag
     2641 tgatgggccg gcacaagccc gagaacatcg tgatcgaaat ggccagagag aaccagacca
     2701 cccagaaggg acagaagaac agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag
     2761 agctgggcag ccagatcctg aaagaacacc ccgtggaaaa cacccagctg cagaacgaga
     2821 agctgtacct gtactacctg cagaatgggc gggatatgta cgtggaccag gaactggaca
     2881 tcaaccggct gtccgactac gatgtggacc atatcgtgcc tcagagcttt ctgaaggacg
     2941 actccatcga caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag agcgacaacg
     3001 tgccctccga agaggtcgtg aagaagatga agaactactg gcggcagctg ctgaacgcca
     3061 agctgattac ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg
     3121 aactggataa ggccggcttc atcaagagac agctggtgga aacccggcag atcacaaagc
     3181 acgtggcaca gatcctggac tcccggatga acactaagta cgacgagaat gacaagctga
     3241 tccgggaagt gaaagtgatc accctgaagt ccaagctggt gtccgatttc cggaaggatt
     3301 tccagtttta caaagtgcgc gagatcaaca actaccacca cgcccacgac gcctacctga
     3361 acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt
     3421 acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag caggaaatcg
     3481 gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc aagaccgaga
     3541 ttaccctggc caacggcgag atccggaagc ggcctctgat cgagacaaac ggcgaaaccg
     3601 gggagatcgt gtgggataag ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc
     3661 cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc aaagagtcta
     3721 tcctgcccaa gaggaacagc gataagctga tcgccagaaa gaaggactgg gaccctaaga
     3781 agtacggcgg cttcgacagc cccaccgtgg cctattctgt gctggtggtg gccaaagtgg
     3841 aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc accatcatgg
     3901 aaagaagcag cttcgagaag aatcccatcg actttctgga agccaagggc tacaaagaag
     3961 tgaaaaagga cctgatcatc aagctgccta agtactccct gttcgagctg gaaaacggcc
     4021 ggaagagaat gctggcctct gccggcgaac tgcagaaggg aaacgaactg gccctgccct
     4081 ccaaatatgt gaacttcctg tacctggcca gccactatga gaagctgaag ggctcccccg
     4141 aggataatga gcagaaacag ctgtttgtgg aacagcacaa gcactacctg gacgagatca
     4201 tcgagcagat cagcgagttc tccaagagag tgatcctggc cgacgctaat ctggacaaag
     4261 tgctgtccgc ctacaacaag caccgggata agcccatcag agagcaggcc gagaatatca
     4321 tccacctgtt taccctgacc aatctgggag cccctgccgc cttcaagtac tttgacacca
     4381 ccatcgaccg gaagaggtac accagcacca aagaggtgct ggacgccacc ctgatccacc
     4441 agagcatcac cggcctgtac gagacacgga tcgacctgtc tcagctggga ggcgacaaaa
     4501 ggccggcggc cacgaaaaag gccggccagg caaaaaagaa aaaggaattc ggcagtggag
     4561 agggcagagg aagtctgcta acatgcggtg acgtcgagga gaatcctggc ccaatgaccg
     4621 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
     4681 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
     4741 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     4801 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     4861 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     4921 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     4981 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     5041 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     5101 ccgcgcccca caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     5161 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgagaattct
     5221 aactagagct cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     5281 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     5341 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     5401 ggggcaggac agcaaggggg aggattggga agagaatagc aggcatgctg gggagcggcc
     5461 gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga
     5521 ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga
     5581 gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg
     5641 cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta
     5701 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg
     5761 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     5821 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     5881 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     5941 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     6001 acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggtc
     6061 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     6121 acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     6181 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     6241 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     6301 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     6361 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     6421 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     6481 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     6541 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     6601 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     6661 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     6721 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     6781 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     6841 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     6901 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     6961 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     7021 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     7081 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     7141 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     7201 tttattgctg ataaatctgg agccggtgag cgtggaagcc gcggtatcat tgcagcactg
     7261 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     7321 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     7381 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     7441 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     7501 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     7561 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     7621 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     7681 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     7741 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     7801 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     7861 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     7921 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     7981 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     8041 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     8101 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     8161 ttacggttcc tggccttttg ctggcctttt gctcacatgt gagggcctat ttcccatgat
     8221 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa ttaatttgac
     8281 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
     8341 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa
     8401 gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg aatataaact
     8461 tgtggtagtg ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact
     8521 tgaaaaagtg gcaccgagtc ggtgcttttt tgttttagag ctagaaatag caagttaaaa
     8581 taaggctagt ccgtttttag cgcgtgcgcc aattctgcag acaaatggct ctagaggtac
     8641 ccgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     8701 tgacgtcaat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     8761 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     8821 acgtcaatga cggtaaatgg cccgcctggc attgtgccca gtacatgacc ttatgggact
     8881 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc
     8941 ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat
     9001 ttatttttta attattttgt gcagcga
//
