LOCUS       sequence-171524-        9000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses sgRNA targeting human AAVS1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9000)
  AUTHORS   Guernet A, Mungamuri SK, Cartier D, Sachidanandam R, Jayaprakash A, 
            Adriouch S, Vezain M, Charbonnier F, Rohkin G, Coutant S, Yao S, 
            Ainani H, Alexandre D, Tournier I, Boyer O, Aaronson SA, Anouar Y, 
            Grumolato L
  TITLE     CRISPR-Barcoding for Intratumor Genetic Heterogeneity Modeling and 
            Functional Analysis of Oncogenic Driver Mutations.
  JOURNAL   Mol Cell. 2016 Aug 4;63(3):526-38. doi: 
            10.1016/j.molcel.2016.06.017. Epub 2016 Jul 21.
  PUBMED    27453044
REFERENCE   2  (bases 1 to 9000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.06.017"; journalName: "Mol Cell"; date: 
            "2016-08-4- 4"; volume: "63"; issue: "3"; pages: "526-38"
COMMENT     Sequence Label: sgAAVS1(A)
FEATURES             Location/Qualifiers
     source          1..9000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          9..236
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      248..257
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             257..322
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             329..349
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             374..4474
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             374..4474
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4475..4522
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4538..4591
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4592..5191
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4592..4611)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     complement(5219..5236)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5225..5432
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5441..5581
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5441..5570
                     /label=AAV2 ITR
     rep_origin      5656..6111
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5743..5762)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5953..5974
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6128..6147)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6247..6269
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6307..6325)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6393..6497
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6498..6566,6567..7358)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6716..6735)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7529..8117
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8018..8037
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        8179..8427
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     8179..8199
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8350..8369
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8448..8523
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        8620..8905
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc
       61 gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc
      121 cttctcctcc gggctgtaat tagctgagca agaggtaagg gtttaaggga tggttggttg
      181 gtggggtatt aatgtttaat tacctggagc acctgcctga aatcactttt tttcaggttg
      241 gaccggtgcc accatggact ataaggacca cgacggagac tacaaggatc atgatattga
      301 ttacaaagac gatgacgata agatggcccc aaagaagaag cggaaggtcg gtatccacgg
      361 agtcccagca gccgacaaga agtacagcat cggcctggac atcggcacca actctgtggg
      421 ctgggccgtg atcaccgacg agtacaaggt gcccagcaag aaattcaagg tgctgggcaa
      481 caccgaccgg cacagcatca agaagaacct gatcggagcc ctgctgttcg acagcggcga
      541 aacagccgag gccacccggc tgaagagaac cgccagaaga agatacacca gacggaagaa
      601 ccggatctgc tatctgcaag agatcttcag caacgagatg gccaaggtgg acgacagctt
      661 cttccacaga ctggaagagt ccttcctggt ggaagaggat aagaagcacg agcggcaccc
      721 catcttcggc aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca
      781 cctgagaaag aaactggtgg acagcaccga caaggccgac ctgcggctga tctatctggc
      841 cctggcccac atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga
      901 caacagcgac gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga
      961 ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg ccagactgag
     1021 caagagcaga cggctggaaa atctgatcgc ccagctgccc ggcgagaaga agaatggcct
     1081 gttcggaaac ctgattgccc tgagcctggg cctgaccccc aacttcaaga gcaacttcga
     1141 cctggccgag gatgccaaac tgcagctgag caaggacacc tacgacgacg acctggacaa
     1201 cctgctggcc cagatcggcg accagtacgc cgacctgttt ctggccgcca agaacctgtc
     1261 cgacgccatc ctgctgagcg acatcctgag agtgaacacc gagatcacca aggcccccct
     1321 gagcgcctct atgatcaaga gatacgacga gcaccaccag gacctgaccc tgctgaaagc
     1381 tctcgtgcgg cagcagctgc ctgagaagta caaagagatt ttcttcgacc agagcaagaa
     1441 cggctacgcc ggctacattg acggcggagc cagccaggaa gagttctaca agttcatcaa
     1501 gcccatcctg gaaaagatgg acggcaccga ggaactgctc gtgaagctga acagagagga
     1561 cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc ccccaccaga tccacctggg
     1621 agagctgcac gccattctgc ggcggcagga agatttttac ccattcctga aggacaaccg
     1681 ggaaaagatc gagaagatcc tgaccttccg catcccctac tacgtgggcc ctctggccag
     1741 gggaaacagc agattcgcct ggatgaccag aaagagcgag gaaaccatca ccccctggaa
     1801 cttcgaggaa gtggtggaca agggcgcttc cgcccagagc ttcatcgagc ggatgaccaa
     1861 cttcgataag aacctgccca acgagaaggt gctgcccaag cacagcctgc tgtacgagta
     1921 cttcaccgtg tataacgagc tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc
     1981 cgccttcctg agcggcgagc agaaaaaggc catcgtggac ctgctgttca agaccaaccg
     2041 gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt gcttcgactc
     2101 cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc ctgggcacat accacgatct
     2161 gctgaaaatt atcaaggaca aggacttcct ggacaatgag gaaaacgagg acattctgga
     2221 agatatcgtg ctgaccctga cactgtttga ggacagagag atgatcgagg aacggctgaa
     2281 aacctatgcc cacctgttcg acgacaaagt gatgaagcag ctgaagcggc ggagatacac
     2341 cggctggggc aggctgagcc ggaagctgat caacggcatc cgggacaagc agtccggcaa
     2401 gacaatcctg gatttcctga agtccgacgg cttcgccaac agaaacttca tgcagctgat
     2461 ccacgacgac agcctgacct ttaaagagga catccagaaa gcccaggtgt ccggccaggg
     2521 cgatagcctg cacgagcaca ttgccaatct ggccggcagc cccgccatta agaagggcat
     2581 cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc acaagcccga
     2641 gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc cagaagggac agaagaacag
     2701 ccgcgagaga atgaagcgga tcgaagaggg catcaaagag ctgggcagcc agatcctgaa
     2761 agaacacccc gtggaaaaca cccagctgca gaacgagaag ctgtacctgt actacctgca
     2821 gaatgggcgg gatatgtacg tggaccagga actggacatc aaccggctgt ccgactacga
     2881 tgtggaccat atcgtgcctc agagctttct gaaggacgac tccatcgaca acaaggtgct
     2941 gaccagaagc gacaagaacc ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa
     3001 gaagatgaag aactactggc ggcagctgct gaacgccaag ctgattaccc agagaaagtt
     3061 cgacaatctg accaaggccg agagaggcgg cctgagcgaa ctggataagg ccggcttcat
     3121 caagagacag ctggtggaaa cccggcagat cacaaagcac gtggcacaga tcctggactc
     3181 ccggatgaac actaagtacg acgagaatga caagctgatc cgggaagtga aagtgatcac
     3241 cctgaagtcc aagctggtgt ccgatttccg gaaggatttc cagttttaca aagtgcgcga
     3301 gatcaacaac taccaccacg cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct
     3361 gatcaaaaag taccctaagc tggaaagcga gttcgtgtac ggcgactaca aggtgtacga
     3421 cgtgcggaag atgatcgcca agagcgagca ggaaatcggc aaggctaccg ccaagtactt
     3481 cttctacagc aacatcatga actttttcaa gaccgagatt accctggcca acggcgagat
     3541 ccggaagcgg cctctgatcg agacaaacgg cgaaaccggg gagatcgtgt gggataaggg
     3601 ccgggatttt gccaccgtgc ggaaagtgct gagcatgccc caagtgaata tcgtgaaaaa
     3661 gaccgaggtg cagacaggcg gcttcagcaa agagtctatc ctgcccaaga ggaacagcga
     3721 taagctgatc gccagaaaga aggactggga ccctaagaag tacggcggct tcgacagccc
     3781 caccgtggcc tattctgtgc tggtggtggc caaagtggaa aagggcaagt ccaagaaact
     3841 gaagagtgtg aaagagctgc tggggatcac catcatggaa agaagcagct tcgagaagaa
     3901 tcccatcgac tttctggaag ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa
     3961 gctgcctaag tactccctgt tcgagctgga aaacggccgg aagagaatgc tggcctctgc
     4021 cggcgaactg cagaagggaa acgaactggc cctgccctcc aaatatgtga acttcctgta
     4081 cctggccagc cactatgaga agctgaaggg ctcccccgag gataatgagc agaaacagct
     4141 gtttgtggaa cagcacaagc actacctgga cgagatcatc gagcagatca gcgagttctc
     4201 caagagagtg atcctggccg acgctaatct ggacaaagtg ctgtccgcct acaacaagca
     4261 ccgggataag cccatcagag agcaggccga gaatatcatc cacctgttta ccctgaccaa
     4321 tctgggagcc cctgccgcct tcaagtactt tgacaccacc atcgaccgga agaggtacac
     4381 cagcaccaaa gaggtgctgg acgccaccct gatccaccag agcatcaccg gcctgtacga
     4441 gacacggatc gacctgtctc agctgggagg cgacaaaagg ccggcggcca cgaaaaaggc
     4501 cggccaggca aaaaagaaaa aggaattcgg cagtggagag ggcagaggaa gtctgctaac
     4561 atgcggtgac gtcgaggaga atcctggccc aatgaccgag tacaagccca cggtgcgcct
     4621 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     4681 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     4741 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     4801 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     4861 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     4921 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     4981 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     5041 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccaca acctcccctt
     5101 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     5161 ctggtgcatg acccgcaagc ccggtgcctg agaattctaa ctagagctcg ctgatcagcc
     5221 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     5281 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     5341 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     5401 gattgggaag agaatagcag gcatgctggg gagcggccgc aggaacccct agtgatggag
     5461 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc
     5521 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag
     5581 gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt
     5641 caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     5701 cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc
     5761 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     5821 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
     5881 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     5941 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct
     6001 attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga
     6061 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca
     6121 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     6181 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     6241 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     6301 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     6361 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     6421 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     6481 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     6541 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     6601 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     6661 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     6721 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     6781 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     6841 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     6901 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     6961 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     7021 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     7081 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     7141 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     7201 ccggtgagcg tggaagccgc ggtatcattg cagcactggg gccagatggt aagccctccc
     7261 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     7321 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     7381 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     7441 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     7501 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     7561 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     7621 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     7681 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     7741 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     7801 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     7861 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     7921 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     7981 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     8041 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     8101 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     8161 ggccttttgc tcacatgtga gggcctattt cccatgattc cttcatattt gcatatacga
     8221 tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta
     8281 caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt
     8341 tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta
     8401 tatatcttgt ggaaaggacg aaacaccgtc ccctccaccc cacagtggtt ttagagctag
     8461 aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
     8521 tgcttttttg ttttagagct agaaatagca agttaaaata aggctagtcc gtttttagcg
     8581 cgtgcgccaa ttctgcagac aaatggctct agaggtaccc gttacataac ttacggtaaa
     8641 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaatag taacgccaat
     8701 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     8761 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     8821 cgcctggcat tgtgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     8881 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
     8941 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
//
