LOCUS       sequence-171528-        9517 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tissue-specific FLP expression plasmid for C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9517)
  AUTHORS   Munoz-Jimenez C, Ayuso C, Dobrzynska A, Torres-Mendez A, Ruiz PC, 
            Askjaer P
  TITLE     An Efficient FLP-Based Toolkit for Spatiotemporal Control of Gene 
            Expression in Caenorhabditis elegans.
  JOURNAL   Genetics. 2017 Aug;206(4):1763-1778. doi: 
            10.1534/genetics.117.201012. Epub 2017 Jun 23.
  PUBMED    28646043
REFERENCE   2  (bases 1 to 9517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.117.201012"; journalName: "Genetics"; date: 
            "2017-08"; volume: "206"; issue: "4"; pages: "1763-1778"
COMMENT     Sequence Label: pBN280
FEATURES             Location/Qualifiers
     source          1..9517
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1881..1905)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2952..2972)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(2980..2997)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2980..2996)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2989..3011)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3324..3346
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3384..3402)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3470..3574
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3575..3643,3644..4435)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3793..3812)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4606..5194
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5095..5114
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5321..5343
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5340..5356
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5340..5356
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    5376..5396
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    6857..6881
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(7030..7041)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tggacaacat ttgaaggata tgcagataca tacgcgttag atacgacggt tgatgtagga
       61 ggagaggtag tcgaggacct cttgggagat gattccgttc caggctgggt aacggatgga
      121 tccctcggcg gatcccttga gttgctcgat gtgttgccac tcctcgattg ggttggtctc
      181 gtccttgagg gcgatcatct ccttggagat tgggtcgtag gcgtagtaac gggagacgag
      241 ggcgaagtag tggtctggga tggcggtgat ttggtgggtg taggtggtac gggcgacggc
      301 ggaggcacgc ttgtcggacc agtttccgac gacgttggtg agctcggtga gtccctgaaa
      361 atttaaatca gattagttag ttagtaaaat catgtttaaa cttaccttca tggagaggaa
      421 ggaggtcatg aggtgacgtc cgatgtggga ctttggtccg ttcttgatgg cgaagatgga
      481 gtatggggcg ttcttcttga gggccttgtt gtaggaacgg acgaggttgt ccttgaggag
      541 ttggtactct tgcttgttgg aggaggagtt tccggtacgg ttgacacgct tgaggactgg
      601 ctcggagtta cggaggaact cgtcgaggta gacgagtggg tcgatacgtc cacgggcgga
      661 gaagaagtag atgtgacggg agacggaggt cttggtctcg gtgacgaggc attggatgat
      721 gactccgagg tacttgtttt ggacgagctt gaaggacttt gggtcgacgt tcttgatgtc
      781 ggagaaacgt ccgcagttga tgaaggtggc gaggaagagg aattggtaga gggtcttggt
      841 cttggtgaaa cgggaggtgt actcgaagga gttgaggatc ttctcggtga tctcccagat
      901 ggactctccc tcggagagga gggccttgag catcttcttg gagtgggagt ttcccttgtc
      961 ggcctcctcg gaggactcga attggagttg gagggaggag acgatgtcgg tgatgtcgga
     1021 ttggtgcttt tgtccgtagt atgggatgat ggtgaactcc caggctggga tgagcttctg
     1081 aaaatttaaa taatcagggt tagttagtat atatatgttt aaacttacct tgagggaggc
     1141 ctcgaggatg gtggcctttt gggtcttgta cttgaattgg agggacttgt tgacgatgtc
     1201 gaaggagagg gagttggaga tgatggtgtt gtaggacatg aaggtggcac gcttgatggc
     1261 ggttccgttg tgggtgatca tccagcagag gtaggtgagc tcggcggcgc agagggcgat
     1321 cttctctccg gatggacgct cgaaacgctc gacgaattga cggacgagga cttttggtgg
     1381 tgtcttacag agaatgtcga attgtggcat ttttggtacc atgattgaag agagcaatgc
     1441 tacctaaaaa ccaaagaaaa tcatgaaatg aggttgagag gggtctttgc tgtgttgatt
     1501 gattgtttta ttatgcgcaa acaaagacac ctcctctttt agatatccga tggcgtggtg
     1561 cttctggaat tcggcacgta atatacaatg gagccgcctg ccacacaatc taatatccgg
     1621 agaaaaaaaa acgaaatgag tcgaataagg cgccactcta tttatttttg ctgtcatggt
     1681 ttccgtggga gaataaatga gcccacggct tgtgatgatg ggaaaagatg agaatgatgt
     1741 cagatttcga atttattgaa gaagaataat gaacgggaac gcatcggagc ttactacgcg
     1801 gccgcatgct acagccggct cctcgagacg tacggtgcgc gcgatgcatt cgaagatctg
     1861 cccactagtg agtcgtatta acccagcttt cttgtacaaa gtggatagaa tcaagcatgc
     1921 tccgcgtttg ctgtactatt tctcgcagag cagttttttt tctcacactt tattattctg
     1981 tactgcccct ccacgctata attttttgca tagagggcca taaaggaaaa cggtacggta
     2041 aaaataatgg gtccttttta ccgttgtgta gattgtttct aacgggatgc aaattttcac
     2101 taaaactctg gaaaactgat ggaattctac gggcagccgg caattttaga tatttgaaga
     2161 atccttcttg atcagtgcca agctttccca tggaccaaag atgtggatcc aagatcaaat
     2221 gcacaggatc tgaactcctt tgttccaaaa acaaggattt cttttctata gtttgaccta
     2281 aatattatag tagaacatat acatttgatg atttcatgaa taatttattg ttaaaaaata
     2341 atacatgtgt taaaaaataa ttctaaaaaa ttaattggag cgtccttggc ggatatggcg
     2401 gattggaagg tcataagtaa ctcgaactaa cctgtaaata taattaattt agaagtaatc
     2461 attttgaaat gtttctaaat ttttgtaaac gtaatcaatg atttacttac aaaatttttc
     2521 gaaatattag tagaaatata aaaaaccagt aaaattcaaa agaggccagc cacacatgca
     2581 tcttacaata cgaatgatgc gttttttgtc gcctcggaga aggaatcaag caggctccgc
     2641 gttttctgta ctatttctcc cagagccgtt tattttgcat agggggtggg tcataaacgg
     2701 agacgttttg gtataaaatt tgccccttct tccaccgttg aaaaaattgg aaaatgaacc
     2761 gggacaggaa aggggatttc aggataatgg ttcaattatc gatgctactg tcatatctga
     2821 gtagtggatt tgagcaaggt ttccaaattt tgagtacaag cgtctagtgc atgtgtcaaa
     2881 aaagtcgggc aaaaattagt tttaatcaat aaggtttaaa gatcagaaat ttttgtctga
     2941 cctaatgggc ccaactttat tatacatagt tgataattca ctggccgtcg ttttacaacg
     3001 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     3061 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     3121 cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     3181 acaccgcata tggtgcactc tcagtgcaat ctgctctgat gccgcatagt taagccagcc
     3241 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     3301 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     3361 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     3421 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     3481 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     3541 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     3601 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     3661 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     3721 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     3781 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     3841 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     3901 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     3961 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     4021 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     4081 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     4141 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     4201 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     4261 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggtc
     4321 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     4381 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     4441 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     4501 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     4561 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     4621 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     4681 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     4741 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     4801 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     4861 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     4921 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     4981 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     5041 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     5101 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     5161 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     5221 gttcctggtc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     5281 tgtggataac cgtattaccg cctttgagtg agctgatacc agcggataac aatttcacac
     5341 aggaaacagc tatgaccatg attacgccaa gctatcaact ttgtatagaa aagttggagg
     5401 gacttccagc tgtctgcgag ccagtccaaa agggagagcc aggatccacc ggagagccag
     5461 gaccagtcgg accaccagga ccatccggac aaccaggaaa cgacggaacc ccaggtaatc
     5521 atttcatagt ttttcaatcc atatctaatc accacatctt tcaggacaac ctggaccaaa
     5581 gggaccacca ggaccagacg gcaagccagg agccgacgga aacccaggac agccaggacc
     5641 agtcggacca ccaggaaccc caggagagcg tggaatctgt ccaaagtact gcgccatcga
     5701 tggaggaatc ttcttcgagg acggaactcg ccgctaagcg gatcgccttt aatttattct
     5761 ttgccttatg cgttgctcaa aataaaacat tatttcatcc aaaataattt tttgaggttt
     5821 tagatgaatt tgatagttta gcttgtcact ttctcctgtg cgaagaaact tgctaagcag
     5881 gtaagcagca tccttatcaa gtttcgacct tatctctcag tagtcgcata cgtcgtatcc
     5941 ctcatctgct ttgttttagc cacttcaagg gaattcagat cactttttct catgtgtttt
     6001 caaatttact tttgtggagt gaccttaatt gaccctctcg tcataagtgg acggtgggta
     6061 gttacttaac aaatttcgag tacaggatag tgtcaatggc atgagacatg tagtagccaa
     6121 gcaattatgg cgaataagtt tcaaaaacca acattctgtc aaatacaaag acctgcaaca
     6181 ttagagaatt cattaaatac atcttcgagg aaatcaaatt ttcccgtaga cgatcccgta
     6241 gacctacttg tacaatatgt tctagatata cactccgaac ttaaaatatc tcgaacattt
     6301 gtgttgaata tttattttaa ttctaggttt ttcaaattcc gcacgatttt tgccatacta
     6361 ctgcccaata gtttacattt attgggcctg gatctgtaag gcacttttgg gagctccctt
     6421 tcttttcctt caaattaccc atacagatct ataaatcaca atttaataca agattttatt
     6481 caagttaaaa acataaaagg ggctttgcgg tggtccccat ttcaccagag aactataaaa
     6541 aatataaatt taacatcaaa tgttgcaatt gattccctct caaagctgct tgaagaaccc
     6601 tgattctgtc aagcctatga agatttaaaa aaaattggga agacccttag ttccaaacaa
     6661 gtgtcgttga cccagtaggc gatattctga aaagtcataa aatggggttg ctaaaaaatt
     6721 ggtcgctaac ttacatttag ctaggaatgt taattggaat aactcataat ttacagtaaa
     6781 attatcaaaa aatagcaaaa atcaatagag gaccgctaca caggcatcca acagtacgag
     6841 ttctgcgttt ttgaattcaa gtttgtacaa aaaagcaggc tctttgagcc aatttatcca
     6901 agtccttgta aaaagtattc gaaaattgtt aacggataaa atgtttatta taatatcaaa
     6961 aacaatttgt cagttgacca ctttttgata tatttgacag aaaccgggat gaattggctc
     7021 aaagttaggg cgcccttcta ttacagggtt tctgataaca aaccggttat taactcccaa
     7081 caagggatga tttcaattca tcatgctcaa attgacccaa attaagttac atgacaaatt
     7141 catcgccctt ttcaactctt cttggctcat catctgttat tctgttccta ttctgtctgc
     7201 accctatacc cctttgcata ctctctcgtc attcctttct tggatagtgt cttcttctcc
     7261 ccagctctgc tacttctatg acttgcgccc gctgcttttc cgctcgttcc tctcctctct
     7321 gacgtcttct tctgcttctt ttctccttcc tttttccctt ctcaatcttc ctctttttcc
     7381 catttctgtc aatcattcga agaagaagaa gaagaagacc ctcattcatt tcattttttt
     7441 ttctgtcggt gtgtgctgct cggttaagag tgagctctct attccacgtc ttcttctttt
     7501 tcttcttcga ttcgaatcaa tcactccacc aaaacgcatt cgtttttggg attcaccccg
     7561 cggttcgcaa taggtttctt tttcaaatta ttagcgttat aaatagaaaa atggtggagt
     7621 ttcaataaaa atgataattt acaaagtgta ttttgattac atgtactcaa aaaggttgaa
     7681 atttttcata ccagttttcc ggaaatccat ctgatatcat tatcgtattt ttctttttaa
     7741 aaatgttttc aaaaaaaaac aaaatatagc tggttatttg gcaccctcta attaccattt
     7801 tcctgtcaca ccacactctt ttccttcttc cacttctttc cgttttcagc cgcttccaac
     7861 caaaccgata tgaaagccga gcaacaacga tcgattccac ccggctcggc gaccttcccg
     7921 tcgcaggtga gactcagaaa actagagaaa ccgctcaact aactcttgat atccgatttc
     7981 attcttttct cttttctttt tgttgaactt ttccatattt ccagatgcca cggccaccac
     8041 caagcaccga acaaggaatc acaacggaat cggagcttgc gaagaaagct caaatcactc
     8101 cgaacgacgt tttagcactt ccgggaatca ctcaaggtat cttgctccct ttggatttca
     8161 gtttaaaaca taaatttagg attcttatgc tccccatctg cgaacatcta taacatcgag
     8221 ttcaccaagt tccaaatccg tgatctggac actgagcaag tgcttttcga gatcgccaaa
     8281 ccggagaacg atcaggagaa tgatgagtcg ccacaggagt cggcaagata cgtgcgttat
     8341 agatttgctc caaacttttt gaaactcaaa acggtcggag caactgtgga attcaaagta
     8401 ggagacatcc caatccatca tttccgaatg atcgaacgtc acttcttcaa agatcgcctt
     8461 ctgaagtgtt ttgactttga attcggattc tgtattccga attcacgaaa caactgtgaa
     8521 catatctatg agttccctca actctctcaa caactcagtg agtcattatt ctaaaaagta
     8581 caattcaaaa gactaatctc tttttcagtg gacgacatga tcaacaatcc aaacgagact
     8641 cgttctgaca gcttctattt cgtcgataac aaactcgtca tgcacaacaa agccgactac
     8701 tcatatgatg cataaatatt taatacaaaa atgttctgga taattattct gtcgaataga
     8761 aaaaaaactc caaatgtgat taaattccaa taattcctgt ctagtttgtt ccttcccctt
     8821 ccccttctca tgttcaatgc attcctaagc ttttcagttc cccccttgtt tctatatttt
     8881 ttcgctgtcc tgtcacactc gctaaaaaca ctaatcacac ggaaatctgt tttcaataaa
     8941 aactccaact ttaactcatt ttcaatttca actgaaagat tttttcatta gagaatgtct
     9001 agaactaggc ccggggaaag ccgggttacc ataagaaaaa aaattacatt ttttctcgaa
     9061 tagtagaaac atattgcaag actatttttc attatttcag aacacacact ggagagagac
     9121 cgtatccatg tgctcaatgt gagaaggcat tcaccgacag ctcaactctt acaaaacatc
     9181 tgagaaccca tactggacat aaaccttatg tttgctcaat ttgtatgatg aaattcacac
     9241 agagtgggaa cttgcatagg cacatgaaaa ctcataaata gtttttccta ttatggaagt
     9301 aatgtttcta aatgaatatt gccattttct gcaaaatatt ttacaatcca caagtctcga
     9361 acaaatttaa tcaatgtctt gttttgatac aatttaacaa attcactgtg gagaggattt
     9421 actataaaaa cgttttacac cagcaaaaaa tcaatagaac atttacatga taaggagaat
     9481 atataaaaag ggggcattat aatttactga tacaata
//
