LOCUS       sequence-171537-        4583 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express zebrafish recoverin2a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Zang J, Keim J, Kastenhuber E, Gesemann M, Neuhauss SC
  TITLE     Recoverin depletion accelerates cone photoresponse recovery.
  JOURNAL   Open Biol. 2015 Aug;5(8). pii: 150086. doi: 10.1098/rsob.150086.
  PUBMED    26246494
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Open 
            Biol."; date: "2015-08"; volume: "5(8). pii: 150086. doi"; pages: " 
            10.1098/rsob.150086"
COMMENT     Sequence Label: recoverin2a
FEATURES             Location/Qualifiers
     source          1..4583
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      58..486
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(145..164)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     355..376
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             830..1624
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(884..903)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1494..1513
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(1642..1710,1711..2502)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1860..1879)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2673..3261
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3162..3181
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3415..3432
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3549..3570
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3585..3590,3591..3608,3609..3615)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3623..3639
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3628..3650
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3647..3663
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3647..3663
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        3681..3699
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3681..3698
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4458..4477)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4459..4477)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4484..4501)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4484..4500)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4493..4515)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggacg
       61 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
      121 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
      181 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
      241 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
      301 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
      361 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
      421 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
      481 cgaattttaa caaaattcag ggcgcaaggg ctgctaaagg aagcggaaca cgtagaaagc
      541 cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta tctggacaag
      601 ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat ggcgatagct
      661 agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg cgccctctgg
      721 taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa ggatctgatg
      781 gcgcagggga tcaagatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
      841 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
      901 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
      961 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     1021 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     1081 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     1141 atcccacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     1201 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     1261 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     1321 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
     1381 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     1441 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     1501 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     1561 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     1621 ctgaattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     1681 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     1741 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     1801 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     1861 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     1921 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     1981 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     2041 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     2101 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     2161 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     2221 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     2281 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     2341 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     2401 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     2461 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     2521 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     2581 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     2641 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     2701 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     2761 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     2821 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     2881 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     2941 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3001 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3061 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3121 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3181 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3241 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3301 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3361 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     3421 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     3481 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     3541 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     3601 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     3661 gaccatgatt acgccaagct atttaggtga cactatagaa tactcaagct atgcatcaag
     3721 cttggtaccg agctcggatc cactagtaac ggccgccagt gtgctggaat tcgccctttt
     3781 ttttttgcgg ccgcttattt cttctggtgc tcgaactgaa taaggtggat agcgtcttca
     3841 ttctccagaa taccttgaat aaactcgccc tcagccaatc gctcgttatc ctttttattg
     3901 aagtacgacc acagtttgtc tgctctcttt tcaggagtgt tctcatcggc tggcagactt
     3961 tcttggtcct ctttgggaat cagcttaaag atagcctggc agatttctgc aacttctgac
     4021 ttggttatgt agccattttt gtcgacatcg aaaagtgaga aggcccactc cagcttgcgc
     4081 tccgtctttc cggttgatgt catatgtaga gcgataatgt attccttgaa atccagcgtt
     4141 ccgtcatcat tggtgtcaaa ggagcggaag acgtgctggg cgtatgaggt ggtgtcacat
     4201 tccggaaaaa aacgttcgta gatcttttta aactcttcag gtgtgatacg gccagacgga
     4261 cactgcttct ggaagttctc gtaccactgc gacagttcgt tctctgagaa tttggtgttg
     4321 agtttgagat cctccaggat ctccttggac atagcactgc ttttggcatt ccccatagct
     4381 agctttttaa gggcgaattc tgcagatatc catcacactg gcggccgctc gagcatgcat
     4441 ctagagggcc caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac
     4501 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     4561 ctttcgccag ctggcgtaat agc
//
