LOCUS       sequence-171538-        4574 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express zebrafish recoverin2b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Zang J, Keim J, Kastenhuber E, Gesemann M, Neuhauss SC
  TITLE     Recoverin depletion accelerates cone photoresponse recovery.
  JOURNAL   Open Biol. 2015 Aug;5(8). pii: 150086. doi: 10.1098/rsob.150086.
  PUBMED    26246494
REFERENCE   2  (bases 1 to 4574)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Open 
            Biol."; date: "2015-08"; volume: "5(8). pii: 150086. doi"; pages: " 
            10.1098/rsob.150086"
COMMENT     Sequence Label: recoverin2b
FEATURES             Location/Qualifiers
     source          1..4574
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(331..349)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(332..349)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(367..383)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(367..383)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(380..402)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    391..407
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(415..421,422..439,440..445))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    460..481
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(598..615)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(769..1357)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(849..868)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1528..2319,2320..2388))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2151..2170
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(2406..3200)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2517..2536)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3127..3146
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     rep_origin      complement(3544..3972)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3654..3675)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3866..3885
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4098..4120
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4112..4129
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4113..4129
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4136..4155
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4136..4154
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggttacgtca caaaacctga agtgattgaa ctcagccagg ccattttcaa actcatcccc
       61 aaagacaagc agacaagtct tcccaacgat gagagcacgc cagagaagag agcagagaaa
      121 ctctgggcca tctttgacaa aaaggacaac gaacgagtgg cagaggggga gttcattgaa
      181 gccatccagt caagcgatga tggtctacgg ctcattcagt atgatcataa atgatgcggc
      241 cgcaaaaaaa aaagggcgaa ttccagcaca ctggcggccg ttactagtgg atccgagctc
      301 ggtaccaagc ttgatgcata gcttgagtat tctatagtgt cacctaaata gcttggcgta
      361 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
      421 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
      481 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
      541 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
      601 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
      661 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
      721 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
      781 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
      841 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
      901 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
      961 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     1021 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     1081 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     1141 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     1201 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     1261 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     1321 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     1381 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     1441 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     1501 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     1561 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     1621 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     1681 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     1741 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     1801 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     1861 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     1921 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     1981 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     2041 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     2101 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     2161 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     2221 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     2281 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     2341 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     2401 tcaattcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga atcgggagcg
     2461 gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc ttcagcaata
     2521 tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg gccacagtcg
     2581 atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc atcgccatgg
     2641 gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga gcctggcgaa cagttcggct
     2701 ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc ggcttccatc
     2761 cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga
     2821 tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca
     2881 aggtgggatg acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc
     2941 gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc cagccacgat
     3001 agccgcgctg cctcgtcctg cagttcattc agggcaccgg acaggtcggt cttgacaaaa
     3061 agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca gccgattgtc
     3121 tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga acctgcgtgc
     3181 aatccatctt gttcaatcat gcgaaacgat cctcatcctg tctcttgatc agatcttgat
     3241 cccctgcgcc atcagatcct tggcggcaag aaagccatcc agtttacttt gcagggcttc
     3301 ccaaccttac cagagggcgc cccagctggc aattccggtt cgcttgctgt ccataaaacc
     3361 gcccagtcta gctatcgcca tgtaagccca ctgcaagcta cctgctttct ctttgcgctt
     3421 gcgttttccc ttgtccagat agcccagtag ctgacattca tccggggtca gcaccgtttc
     3481 tgcggactgg ctttctacgt gttccgcttc ctttagcagc ccttgcgccc tgaattttgt
     3541 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     3601 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     3661 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     3721 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     3781 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     3841 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     3901 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     3961 tacagggcgc gtccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     4021 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     4081 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata
     4141 cgactcacta tagggcgaat tgggccctct agatgcatgc tcgagcggcc gccagtgtga
     4201 tggatatctg cagaattcgc ccttaaaaac atatgggaaa cgcacagagc ggagggattc
     4261 ccagagaaat cctggatgac ctgaagctca ccaccaggtt ctccgaatct gagatcaccc
     4321 agtggtatga gaacttccag aagcagtgtc ctacaggtcg catcacactg cagcagttcg
     4381 aggagatcta cggtaaattc ttccctgaca gcgatgccac cacttacgcc caacacgttt
     4441 tccgctcctt tgatgccaat gacgatggaa cgttagactt taaggagtac gttgtggctc
     4501 ttcacatgac cccctcgggc aagtccaccc taaagctgga gtgggccttc tcactgtttg
     4561 atgtggacaa gaac
//
