LOCUS       sequence-171561-        2919 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducibile Tra Promoter driving sfGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Scott SR, Hasty J
  TITLE     Quorum Sensing Communication Modules for Microbial Consortia.
  JOURNAL   ACS Synth Biol. 2016 Sep 16;5(9):969-77. doi: 
            10.1021/acssynbio.5b00286. Epub 2016 May 19.
  PUBMED    27172092
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00286"; journalName: "ACS Synth Biol"; date: 
            "2016-09-16- 16"; volume: "5"; issue: "9"; pages: "969-77"
COMMENT     Sequence Label: Bsrs074-Ptra
FEATURES             Location/Qualifiers
     source          1..2919
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     83..100
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     84..100
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    116..215
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             369..1082
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1112..1129)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(1191..1290)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1307..1326)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1308..1326)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1331..1347)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1331..1347)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1460..1564
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1565..1633,1634..2425)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1783..1802)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tgtgacccag acctcgcaaa atgagaccag ataagcctta tcagataaca gcgccctttt
       61 ggcgtctttt tgagcaccta ggtgtaaaac gacggccagt cttaagctcg ggccccaaat
      121 aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgatg agcaatgctt
      181 ttttataatg ccaactttgt acaaaaaagc aggctccgaa ttggtatcac gaggcagaat
      241 ttcagataaa aaaaatcctt agctttcgct aaggatctga agtggaattc atgagatctc
      301 tacgtgcaga tctgcacata gccacaccct gaatgagatg ttttctctcc gctaaaggag
      361 gtgacaatat gcgtaaaggc gaagagctgt tcactggtgt cgtccctatt ctggtggaac
      421 tggatggtga tgtcaacggt cataagtttt ccgtgcgtgg cgagggtgaa ggtgacgcaa
      481 ctaatggtaa actgacgctg aagttcatct gtactactgg taaactgccg gtaccttggc
      541 cgactctggt aacgacgctg acttatggtg ttcagtgctt tgctcgttat ccggaccata
      601 tgaagcagca tgacttcttc aagtccgcca tgccggaagg ctatgtgcag gaacgcacga
      661 tttcctttaa ggatgacggc acgtacaaaa cgcgtgcgga agtgaaattt gaaggcgata
      721 ccctggtaaa ccgcattgag ctgaaaggca ttgactttaa agaagacggc aatatcctgg
      781 gccataagct ggaatacaat tttaacagcc acaatgttta catcaccgcc gataaacaaa
      841 aaaatggcat taaagcgaat tttaaaattc gccacaacgt ggaggatggc agcgtgcagc
      901 tggctgatca ctaccagcaa aacactccaa tcggtgatgg tcctgttctg ctgccagaca
      961 atcactatct gagcacgcaa agcgttctgt ctaaagatcc gaacgagaaa cgcgatcata
     1021 tggttctgct ggagttcgta accgcagcgg gcatcacgca tggtatggat gaactgtaca
     1081 aatgaggatc ctaactcgct cctcaggctt cctcgctcac tgactcgctg cgctcggtcg
     1141 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatcaattcg acccagcttt
     1201 cttgtacaaa gttggcatta taaaaaataa ttgctcatca atttgttgca acgaacaggt
     1261 cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc
     1321 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg
     1381 ttacattgca caagataaaa atatatcatc atgcctcctc tagacttaga cgtcaggtgg
     1441 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     1501 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     1561 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     1621 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     1681 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     1741 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     1801 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     1861 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     1921 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     1981 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     2041 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     2101 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     2161 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     2221 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     2281 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     2341 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     2401 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     2461 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatcc
     2521 tcgaggagcg cctcagcgcg ccgtagcgtc gataaaaatt acgggctggg gcgaaactac
     2581 catctgttcg aaaaggtccg taaatgggcc tacagagcga ttcgtcaggg ctggcctgta
     2641 ttctcacaat ggcttgatgc cgttatccag cgtgtcgaaa tgtacaacgc ttcgcttccc
     2701 gttccgcttt ctccggctga atgtcgggct attggcaaga gcattgcgaa atatacacac
     2761 aggaaattct caccagaggg attttccgct gtacaggccg ctcgcggtcg caagggcgga
     2821 actaaatcta agcgcgcagc agttcctaca tcagcacgtt cgctgaaacc gtgggaggca
     2881 ttaggcatca gtcgagcgac gtactaccga aaattaaaa
//
