LOCUS       sequence-171569-       11131 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses PP7-VP64 fused to DHFR domain on N-terminus in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11131)
  AUTHORS   Maji B, Moore CL, Zetsche B, Volz SE, Zhang F, Shoulders MD, 
            Choudhary A
  TITLE     Multidimensional chemical control of CRISPR-Cas9.
  JOURNAL   Nat Chem Biol. 2017 Jan;13(1):9-11. doi: 10.1038/nchembio.2224. Epub
            2016 Oct 31.
  PUBMED    27820801
REFERENCE   2  (bases 1 to 11131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2224"; journalName: "Nat Chem Biol"; date: 
            "2017-01"; volume: "13"; issue: "1"; pages: "9-11"
COMMENT     Sequence Label: DHFR-PP7-VP64_T2A_GFP
FEATURES             Location/Qualifiers
     source          1..11131
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        84..463
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        465..663
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     618..638
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             681..861
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    908..1033
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1526..1759
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1944..1988
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2286..2403
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2528..3706
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2759..3697
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3654..3674
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3719..4195
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="MISLIAALAVDYVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRH
                     TWESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVIEQFL
                     PKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     CDS             4658..4678
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4703..4852
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             4874..4927
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4928..4930,4931..5644)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(4928..4930,4931..5641)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4970..4991)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5231..5250)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5578..5599
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5669..6257
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5722..5742)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6140..6151)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6260..6276)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6261..6277)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6782..6962
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6988..7005)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6994..7218
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7264..7692
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7351..7370)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7561..7582
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7701..7721)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7706..8035
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7886..8021
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7948..7967
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8083..8130
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8149..8523
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8653..8774
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8690..8709)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8744..8763
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8823..8839)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8823..8839)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8836..8858)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8847..8863
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8871..8877,8878..8895,8896..8901))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8916..8937
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9054..9071)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9225..9813)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9305..9324)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9984..10775,10776..10844))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10607..10626
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10845..10949)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11024..11043)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
       61 tgtacgggcc agatatacgc gttgacattg attattgact agttattaat agtaatcaat
      121 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
      181 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
      241 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
      301 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
      361 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
      421 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
      481 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
      541 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
      601 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
      661 cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
      721 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
      781 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
      841 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
      901 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
      961 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     1021 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     1081 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     1141 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     1201 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     1261 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     1321 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     1381 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     1441 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     1501 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     1561 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     1621 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     1681 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     1741 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     1801 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     1861 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     1921 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     1981 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     2041 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     2101 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     2161 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     2221 gattagtgaa cggatcggca ctgcgtgcgc caattctgca gacaaatggc agtattcatc
     2281 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     2341 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     2401 tttcgggttt attacaggga cagcagagat ccagtttggt taattaatgc aaagatggat
     2461 aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct tgaaaggagt
     2521 gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     2581 gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg
     2641 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     2701 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt
     2761 aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc
     2821 ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag
     2881 tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga
     2941 ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct
     3001 cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt
     3061 tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt
     3121 tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg
     3181 cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct
     3241 ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc
     3301 ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa
     3361 atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc
     3421 ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca
     3481 cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta
     3541 tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt
     3601 gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc
     3661 tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgacgta cggccaccat
     3721 gatcagtctg attgcggcgt tagcggtaga ttacgttatc ggcatggaaa acgccatgcc
     3781 gtggaacctg cctgccgatc tcgcctggtt taaacgcaac accttaaata aacccgtgat
     3841 tatgggccgc catacctggg aatcaatcgg tcgtccgttg ccaggacgca aaaatattat
     3901 cctcagcagt caaccgagta cggacgatcg cgtaacgtgg gtgaagtcgg tggatgaagc
     3961 catcgcggcg tgtggtgacg taccagaaat catggtgatt ggcggcggtc gcgttattga
     4021 acagttcttg ccaaaagcgc aaaaactgta tctgacgcat atcgacgcag aagtggaagg
     4081 cgacacccat ttcccggatt acgagccgga tgactgggaa tcggtattca gcgaattcca
     4141 cgatgctgat gcgcagaact ctcacagcta ttgctttgag attctggagc ggcgaggtgg
     4201 aggaggttca ggaggtggag gctctggagg tggaggcagc tccaaaacca tcgttctttc
     4261 ggtcggcgag gctactcgca ctctgactga gatccagtcc accgcagacc gtcagatctt
     4321 cgaagagaag gtcgggcctc tggtgggtcg gctgcgcctc acggcttcgc tccgtcaaaa
     4381 cggagccaag accgcgtatc gcgtcaacct aaaactggat caggcggacg tcgttgattc
     4441 cggacttccg aaagtgcgct acactcaggt atggtcgcac gacgtgacaa tcgttgcgaa
     4501 tagcaccgag gcctcgcgca aatcgttgta cgatttgacc aagtccctcg tcgcgacctc
     4561 gcaggtcgaa gatcttgtcg tcaaccttgt gccgctgggc cgtagcgctg gaggaggtgg
     4621 aagcggagga ggaggaagcg gaggaggagg tagcggacct aagaaaaaga ggaaggtggc
     4681 ggccgctgga tccggacggg ctgacgcatt ggacgatttt gatctggata tgctgggaag
     4741 tgacgccctc gatgattttg accttgacat gcttggttcg gatgcccttg atgactttga
     4801 cctcgacatg ctcggcagtg acgcccttga tgatttcgac ctggacatgc tgattaacgc
     4861 tagcggcagt ggagagggca gaggaagtct gctaacatgc ggtgacgtcg aggagaatcc
     4921 tggcccagtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     4981 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     5041 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     5101 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     5161 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     5221 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     5281 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     5341 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     5401 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     5461 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     5521 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     5581 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     5641 gtaagaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
     5701 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     5761 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     5821 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     5881 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     5941 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     6001 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     6061 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     6121 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     6181 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     6241 ttgggccgcc tccccgcatc gataccgtcg acctcgagac ctagaaaaac atggagcaat
     6301 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga
     6361 ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc
     6421 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     6481 ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     6541 ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta
     6601 caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg
     6661 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg
     6721 gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc cggactgtac
     6781 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     6841 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     6901 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     6961 cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     7021 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     7081 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     7141 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     7201 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     7261 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     7321 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     7381 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     7441 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     7501 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     7561 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     7621 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     7681 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     7741 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     7801 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     7861 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     7921 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     7981 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     8041 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
     8101 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg
     8161 accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc
     8221 gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac
     8281 gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc
     8341 tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg
     8401 aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg
     8461 gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac
     8521 tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     8581 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     8641 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     8701 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     8761 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     8821 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     8881 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     8941 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     9001 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     9061 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     9121 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     9181 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     9241 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     9301 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     9361 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     9421 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     9481 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     9541 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     9601 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     9661 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     9721 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     9781 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     9841 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     9901 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     9961 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    10021 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    10081 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    10141 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    10201 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    10261 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    10321 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    10381 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    10441 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    10501 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    10561 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    10621 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    10681 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    10741 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    10801 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    10861 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    10921 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    10981 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
    11041 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
    11101 cgagcaaaat ttaagctaca acaaggcaag g
//
