LOCUS       sequence-171572-        8797 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral expression of NoBody.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8797)
  AUTHORS   D'Lima NG, Ma J, Winkler L, Chu Q, Loh KH, Corpuz EO, Budnik BA, 
            Lykke-Andersen J, Saghatelian A, Slavoff SA
  TITLE     A human microprotein that interacts with the mRNA decapping complex.
  JOURNAL   Nat Chem Biol. 2016 Dec 5. doi: 10.1038/nchembio.2249.
  PUBMED    27918561
REFERENCE   2  (bases 1 to 8797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2016 Dec 5. doi: 10.1038/nchembio.2249."
COMMENT     Sequence Label: FLAG LOC550643 pFCPGW
FEATURES             Location/Qualifiers
     source          1..8797
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        84..463
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        465..663
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             681..861
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    908..1033
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1526..1759
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1944..1988
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2286..2403
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2462..2765
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2766..2969
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2966..2990
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3067..3090
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    3335..3923
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3388..3408)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3806..3817)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3926..3942)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3927..3943)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4448..4628
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4654..4671)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4660..4884
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4930..5358
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5017..5036)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5227..5248
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5367..5387)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5372..5701
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5552..5687
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5614..5633
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5749..5796
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5815..6189
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6319..6440
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6356..6375)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6410..6429
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6489..6505)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6489..6505)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6502..6524)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6513..6529
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6537..6543,6544..6561,6562..6567))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6582..6603
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6720..6737)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6891..7479)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6971..6990)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7650..8441,8442..8510))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8273..8292
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8511..8615)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8690..8709)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
       61 tgtacgggcc agatatacgc gttgacattg attattgact agttattaat agtaatcaat
      121 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
      181 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
      241 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
      301 aactgcccac ttggcagtac atcaagtgta tcatatgcca agcacgcccc ctattgacgt
      361 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
      421 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
      481 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
      541 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
      601 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
      661 cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
      721 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
      781 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
      841 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
      901 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
      961 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     1021 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     1081 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     1141 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     1201 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     1261 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     1321 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     1381 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     1441 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     1501 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     1561 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     1621 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     1681 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     1741 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     1801 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     1861 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     1921 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     1981 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     2041 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     2101 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     2161 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     2221 gattagtgaa cggatcggca ctgcgtgcgc caattctgca gacaaatggc agtattcatc
     2281 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     2341 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     2401 tttcgggttt attacaggga cagcagagat ccagtttggt taattaagct tgggagttcc
     2461 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     2521 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     2581 aatgggtgga gcatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     2641 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     2701 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     2761 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     2821 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     2881 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     2941 tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac
     3001 gccatccacg ctgttttgac ctccatagaa gacaccgact ctagaggatc cttcgaaacc
     3061 ggtatggact acaaggatga cgacgataag ggagaccaac cttgtgcctc cgggagatcc
     3121 actctcccac ctggaaacgc acgggaagcc aagcctccaa aaaagcgctg cctcctcgct
     3181 ccgcgttggg attatccgga aggaactccc aacggaggta gtaccactct accctccgca
     3241 cctcctcctg catcagccgg cctgaagtcg caccctcctc ctccggagaa gtagggcgcg
     3301 cccctgcagg gaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     3361 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     3421 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     3481 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     3541 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     3601 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     3661 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     3721 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     3781 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     3841 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     3901 ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagacctag aaaaacatgg
     3961 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca
     4021 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta
     4081 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     4141 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt
     4201 ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga cctttggatg
     4261 gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa
     4321 cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt
     4381 agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga
     4441 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     4501 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     4561 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     4621 ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     4681 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     4741 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     4801 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     4861 ctggggatgc ggcgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     4921 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     4981 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     5041 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     5101 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     5161 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     5221 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     5281 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     5341 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     5401 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     5461 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     5521 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     5581 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     5641 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     5701 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa
     5761 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc
     5821 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc
     5881 tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc
     5941 cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc
     6001 ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg
     6061 tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg
     6121 gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag
     6181 caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     6241 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     6301 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     6361 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     6421 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     6481 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     6541 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     6601 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     6661 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     6721 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6781 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     6841 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6901 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6961 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7021 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7081 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7141 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7201 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7261 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7321 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7381 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7441 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7501 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7561 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7621 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7681 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     7741 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     7801 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     7861 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     7921 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     7981 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8041 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8101 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8161 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8221 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     8281 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     8341 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     8401 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     8461 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8521 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     8581 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     8641 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
     8701 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     8761 agtgcgcgag caaaatttaa gctacaacaa ggcaagg
//
