LOCUS       sequence-171778-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor Vector containing ZNF277 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF277
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..29
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 atgcaggaag accgtgatgg gagctgcagc acagtcgggg gtgtaggtta tggggacagt
       61 aaggattgta tcctggagcc gctttccctg ccagaaagtc caggtggcac caccacttta
      121 gaaggttctc catctgtgcc ttgtattttc tgtgaagaac attttcctgt ggctgaacaa
      181 gacaaacttc tgaagcacat gattattgag cataagattg tcatagctga tgtcaagttg
      241 gttgctgatt tccaaaggta cattttatat tggaggaaaa ggttcactga acagcccatc
      301 acagattttt gtagtgtaat aagaattaat tccactgctc catttgaaga acaagagaat
      361 tattttttgt tatgtgacgt tttaccagaa gatagaattc ttagagaaga gcttcagaaa
      421 cagagactga gagaaattct ggaacaacag cagcaagaac gaaatgatac caattttcat
      481 ggcgtttgta tgttttgcaa tgaagaattc cttggaaaca gatctgttat tttgaaccac
      541 atggccagag aacatgcttt caacattgga ttgccagaca acattgtaaa ctgcaatgaa
      601 tttttgtgta cattacagaa aaagcttgac aatttgcagt gcttgtactg tgagaagacc
      661 ttcagggaca aaaatacact taaagatcac atgaggaaaa aacagcatcg taagattaat
      721 cctaagaaca gagaatatga cagattttat gtcatcaatt atttggtaag gctttctttt
      781 cataacttaa aatttgattg cagtactgca aacaaaatct ttctgaggac caactaa
//
