LOCUS       sequence-17180-e         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Sheff MA, Thorn KS
  TITLE     Optimized cassettes for fluorescent protein tagging in Saccharomyces
            cerevisiae.
  JOURNAL   Yeast 2004 Jun;21(8):661-70.
  PUBMED    15197731
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "2004-06"; volume: "21(8)"; pages: "661-70"
COMMENT     Sequence Label: pKT0174
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(48..764)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYIYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGIDELYK"
ORIGIN
        1 taaaatcata aatcataaga aattcgctta tttagaagtg gcgcgcctta tttgtacaat
       61 tcatcaatac catgggtaat accagcagca gtaacaaatt ctaacaagac catgtggtct
      121 ctcttttcgt ttggatcttt ggataaggca gattgagtgg ataagtaatg gttgtctggt
      181 aacaagactg gaccatcacc aattggagta ttttgttgat aatggtcagc taattgaaca
      241 gaaccatctt caatgttgtg tctaattttg aagttagctt tgataccatt cttttgtttg
      301 tcagcagtga tgtaaacatt gtgagagtta taaatgtatt ccaatttgtg acctaaaatg
      361 ttaccatctt ctttaaaatc aatacctttt aattcgattc tattaactaa ggtatcacct
      421 tcaaacttga cttcagctct ggtcttgtag ttaccgtcat ctttgaaaaa aatagttctt
      481 tcttgaacat aaccttctgg catggcagac ttgaaaaagt catgttgttt catatgatct
      541 gggtatctag aaaaacattg aacaccccaa gttaaagtag tgactaaggt tggccatgga
      601 actggcaatt taccagtagt acaaataaat tttaaggtca atttaccgta agtagcatca
      661 ccttcacctt caccggagac agaaaatttg tgaccattaa catcaccatc taattcaacc
      721 aaaattggga caacaccagt gaataattct tcacctttag acatgttaat taaaccagca
      781 ccgtcaccga tccgtcgacc tgcagcgtac g
//
