LOCUS       sequence-171849-         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor Vector containing ZNF146 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF146
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             257..265
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 atgtcacacc tcagccagca gaaaatttac agtggggaaa acccctttgc ctgtaaggta
       61 tgtggaaaag tcttcagcca caaatcaaac ctcactgagc atgagcattt tcacacgaga
      121 gagaaacctt ttgaatgtaa cgagtgtgga aaagccttta gccaaaagca gtatgtcatt
      181 aaacatcaga acacccatac tggcgagaag cttttcgaat gtaatgaatg tggaaaatca
      241 tttagccaga aggaaaacct ccttacgcac cagaaaattc acactggaga aaaacctttt
      301 gagtgtaaag attgcgggaa agctttcatt cagaagtcaa acctcatcag acaccagaga
      361 actcacacag gagagaagcc ctttgtatgt aaggagtgtg gaaaaacctt cagtggcaaa
      421 tccaacctta ctgagcatga gaaaatccat attggagaga agccttttaa atgtagtgaa
      481 tgtggaacag cctttggcca gaagaagtac ctcataaaac atcagaacat tcacactgga
      541 gagaaaccct atgaatgtaa cgaatgtgga aaagccttct ctcagcgaac atcacttatt
      601 gtacatgtga ggattcattc aggtgataaa ccttacgaat gcaatgtttg tggaaaagcc
      661 ttctctcaga gctcatctct cactgtgcat gtgagaagcc atacagggga gaagccctat
      721 ggttgtaatg aatgtgggaa agctttctct cagttctcaa cccttgctct gcatttgaga
      781 atacacacag gtaagaagcc ttatcagtgc agtgaatgtg ggaaagcttt cagccagaag
      841 tcacaccaca ttagacacca gaaaattcat actcactaa
//
