LOCUS       sequence-17188-e         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Sheff MA, Thorn KS
  TITLE     Optimized cassettes for fluorescent protein tagging in Saccharomyces
            cerevisiae.
  JOURNAL   Yeast 2004 Jun;21(8):661-70.
  PUBMED    15197731
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "2004-06"; volume: "21(8)"; pages: "661-70"
COMMENT     Sequence Label: pKT0127
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..745
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGIDELY"
ORIGIN
        1 cgacgggatc ggtgacggtg ctggtttaat taacatgtct aaaggtgaag aattattcac
       61 tggtgttgtc ccaattttgg ttgaattaga tggtgatgtt aatggtcaca aattttctgt
      121 ctccggtgaa ggtgaaggtg atgctactta cggtaaattg accttaaaat ttatttgtac
      181 tactggtaaa ttgccagttc catggccaac cttagtcact actttaactt atggtgttca
      241 atgtttttct agatacccag atcatatgaa acaacatgac tttttcaagt ctgccatgcc
      301 agaaggttat gttcaagaaa gaactatttt tttcaaagat gacggtaact acaagaccag
      361 agctgaagtc aagtttgaag gtgatacctt agttaataga atcgaattaa aaggtattga
      421 ttttaaagaa gatggtaaca ttttaggtca caaattggaa tacaactata actctcacaa
      481 tgtttacatc atggctgaca aacaaaagaa tggtatcaaa gttaacttca aaattagaca
      541 caacattgaa gatggttctg ttcaattagc tgaccattat caacaaaata ctccaattgg
      601 tgatggtcca gtcttgttac cagacaacca ttacttatcc actcaatctg ccttatccaa
      661 agatccaaac gaaaagagag accacatggt cttgttagaa tttgttactg ctgctggtat
      721 tacccatggt attgatgaat tgtac
//
