LOCUS       sequence-17190-e         766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Sheff MA, Thorn KS
  TITLE     Optimized cassettes for fluorescent protein tagging in Saccharomyces
            cerevisiae.
  JOURNAL   Yeast 2004 Jun;21(8):661-70.
  PUBMED    15197731
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "2004-06"; volume: "21(8)"; pages: "661-70"
COMMENT     Sequence Label: pKT0209
FEATURES             Location/Qualifiers
     source          1..766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..765
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGIDELYK"
ORIGIN
        1 gcnnnntacg ctgcaggtcg acggatcggt gacggtgctg gtttaattaa catgtctaaa
       61 ggtgaagaat tattcactgg tgttgtccca attttggttg aattagatgg tgatgttaat
      121 ggtcacaaat tttctgtctc cggtgaaggt gaaggtgatg ctacttacgg taaattgacc
      181 ttaaaattta tttgtactac tggtaaattg ccagttccat ggccaacctt agtcactact
      241 ttaacttatg gtgttcaatg tttttctaga tacccagatc atatgaaaca acatgacttt
      301 ttcaagtctg ccatgccaga aggttatgtt caagaaagaa ctattttttt caaagatgac
      361 ggtaactaca agaccagagc tgaagtcaag tttgaaggtg ataccttagt taatagaatc
      421 gaattaaaag gtattgattt taaagaagat ggtaacattt taggtcacaa attggaatac
      481 aactataact ctcacaatgt ttacatcatg gctgacaaac aaaagaatgg tatcaaagtt
      541 aacttcaaaa ttagacacaa cattgaagat ggttctgttc aattagctga ccattatcaa
      601 caaaatactc caattggtga tggtccagtc ttgttaccag acaaccatta cttatccact
      661 caatctgcct tatccaaaga tccaaacgaa aagagagacc acatggtctt gttagaattt
      721 gttactgctg ctggtattac ccatggtatt gatgaattgt acaaat
//
