LOCUS       sequence-17191-e         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Sheff MA, Thorn KS
  TITLE     Optimized cassettes for fluorescent protein tagging in Saccharomyces
            cerevisiae.
  JOURNAL   Yeast 2004 Jun;21(8):661-70.
  PUBMED    15197731
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "2004-06"; volume: "21(8)"; pages: "661-70"
COMMENT     Sequence Label: pKT0140
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..760
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGIDELYK"
ORIGIN
        1 gtacgctgca ggtcgacgga tcggtgacgg tgctggttta attaacatgt ctaaaggtga
       61 agaattattc actggtgttg tcccaatttt ggttgaatta gatggtgatg ttaatggtca
      121 caaattttct gtctccggtg aaggtgaagg tgatgctact tacggtaaat tgaccttaaa
      181 atttatttgt actactggta aattgccagt tccatggcca accttagtca ctactttagg
      241 ttatggtttg atgtgttttg ctagataccc agatcatatg aaacaacatg actttttcaa
      301 gtctgccatg ccagaaggtt atgttcaaga aagaactatt tttttcaaag atgacggtaa
      361 ctacaagacc agagctgaag tcaagtttga aggtgatacc ttagttaata gaatcgaatt
      421 aaaaggtatt gattttaaag aagatggtaa cattttaggt cacaaattgg aatacaacta
      481 taactctcac aatgtttaca tcatggctga caaacaaaag aatggtatca aagttaactt
      541 caaaattaga cacaacattg aagatggttc tgttcaatta gctgaccatt atcaacaaaa
      601 tactccaatt ggtgatggtc cagtcttgtt accagacaac cattacttat cctatcaatc
      661 tgccttatcc aaagatccaa acgaaaagag agaccacatg gtcttgttag aatttgttac
      721 tgctgctggt attacccatg gtattgatga attgtacaaa
//
