LOCUS       sequence-17216-e         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Janicki SM, Tsukamoto T, Salghetti SE, Tansey WP, Sachidanandam R, 
            Prasanth KV, Ried T, Shav-Tal Y, Bertrand E, Singer RH, Spector DL
  TITLE     From silencing to gene expression: real-time analysis in single 
            cells.
  JOURNAL   Cell 2004 Mar 5;116(5):683-98.
  PUBMED    15006351
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell"; 
            date: "2004-03-5"; volume: "116(5)"; pages: "683-98"
COMMENT     Sequence Label: CMV-dH3-YFP
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      405..414
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(456..477)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ctgctcgcaa atcgactggt ggaaaggcgc cacgcnnaca actggctact aaggccgctc
       61 gcaagagtgc tccagccacc ggaggtgtga agaagcccca ccgctatcgc cctggaaccg
      121 tggccttgcg tgaaattcgt cgctaccaaa agagcaccga gcttctaatc cgcaagctgc
      181 ctttccagcg tctggtgcgt gaaatcgctc aggactttaa gacggacttg cgattccaga
      241 gctcggcggt tatggctctg caggaagcta gcgaagccta cctggtcggt ctcttcgaag
      301 ataccaactt gtgtgccatt catgccaagc gtgtcaccat aatgcccaaa gacatccagt
      361 tagcgcgacg cattcgcggc gagcgtgctc agcggccgtc ggtcgccacc atggtgagca
      421 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
      481 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
      541 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
      601 ccttcngcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact
      661 tcttcaagtc cgccatgccc gaangctacg tccaggagcg caccatcttc ntcanggacg
      721 acggcaacta ca
//
