LOCUS       sequence-172160-        1722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor Vector containing ZNF10 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF10
FEATURES             Location/Qualifiers
     source          1..1722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..225
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
ORIGIN
        1 atggatgcta agtcactaac tgcctggtcc cggacactgg tgaccttcaa ggatgtattt
       61 gtggacttca ccagggagga gtggaagctg ctggacactg ctcagcagat cgtgtacaga
      121 aatgtgatgc tggagaacta taagaacctg gtttccttgg gttatcagct tactaagcca
      181 gatgtgatcc tccggttgga gaagggagaa gagccctggc tggtggagag agaaattcac
      241 caagagaccc atcctgattc agagactgca tttgaaatca aatcatcagt ttccagcagg
      301 agcattttta aagataagca atcctgtgac attaaaatgg aaggaatggc aaggaatgat
      361 ctctggtatt tgtcattaga agaagtctgg aaatgtagag accagttaga caagtatcag
      421 gaaaacccag agagacattt gaggcaagtg gcattcaccc aaaagaaagt acttactcag
      481 gagagagtct ctgaaagtgg taaatatggg ggaaactgtc ttcttcctgc tcagctagta
      541 ctgagagagt atttccataa acgtgactca catactaaaa gtttaaaaca tgatttagtt
      601 cttaatggtc atcaggacag ttgtgcaagt aacagtaatg aatgtggtca aactttctgt
      661 caaaacattc accttattca gtttgcaaga actcacacag gtgataaatc ctacaaatgc
      721 cctgataatg acaactctct tactcatggt tcatctcttg gtatatcaaa gggcatacat
      781 agagagaaac cctatgaatg taaggaatgt ggaaaattct tcagctggcg ctctaatctt
      841 actaggcatc agcttattca tactggagaa aaaccgtatg agtgtaaaga atgtggaaag
      901 tctttcagcc ggagttctca cctcattgga catcaaaaga cccatactgg tgaggaaccc
      961 tatgaatgta aagaatgtgg aaaatccttc agctggttct ctcaccttgt tactcatcag
     1021 agaactcata caggagacaa actgtacaca tgtaatcagt gtgggaaatc ttttgttcat
     1081 agctctaggc ttattagaca ccagaggaca catactggag agaaacccta tgaatgtcct
     1141 gaatgtggga aatctttcag acagagcaca catctcattc tgcatcagag aacccatgtg
     1201 agagtgaggc cctatgaatg caatgaatgt ggaaagtctt acagccagag atctcacctt
     1261 gttgtgcatc atagaattca cactggacta aaaccttttg agtgtaagga ttgtggaaaa
     1321 tgttttagtc gaagctctca cctttattca catcaaagaa cccacactgg agagaaacca
     1381 tatgagtgtc atgattgtgg aaaatctttc agccagagtt ctgcccttat tgtgcatcag
     1441 aggatacaca ctggagagaa accatatgaa tgctgtcagt gtgggaaagc cttcatccgg
     1501 aagaatgacc tcattaagca ccagagaatt catgttggag aagagaccta taaatgtaat
     1561 caatgtggca ttatcttcag ccagaactct ccatttatag ttcatcaaat agctcacact
     1621 ggagagcagt tcttaacatg caatcaatgt gggacagcgc ttgttaatac ctctaacctt
     1681 attggatacc agacaaatca tattagagaa aatgcttact aa
//
