LOCUS       sequence-17218-e         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Roberts CW, Shutter JR, Korsmeyer SJ
  TITLE     Hox11 controls the genesis of the spleen.
  JOURNAL   Nature 1994 Apr 21;368(6473):747-9.
  PUBMED    7908720
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature"; 
            date: "1994-04-21"; volume: "368(6473)"; pages: "747-9"
COMMENT     Sequence Label: 3063 pBS Hox11 genomic
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..26
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10..26
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(625..643)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(664..680)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(664..680)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(677..699)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    688..704
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(712..718,719..736,737..742))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    757..778
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(895..912)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggcggccgct ctagaactag tggatccccc gggctgcagg aattcgatgg aacgtgaggc
       61 cgagaggcag caggcgaacc gcatcctcct gcagctgcag caggaagcct tccagaagag
      121 cctggcccag ccgctgcctg cagacccact gtgcgtgcac aactcctcgc tcttcgccct
      181 gcagaacctg cagccgtggt ctgacgactc caccaaaatc accagcgtca cgtccgtggc
      241 ttcggcctgc gagtgaggac ccaaggcccg ttgaggactt tccggagaac cagaactctc
      301 gacacccttt ctgactgcac gcaggaggga aatggggggc ttctcagcaa ggctcccaag
      361 caccgcctcc actccccagc ggacacttcc tgtcttcggt ggaagagggc tggggataca
      421 ggcagagaca tcttcccaga agcctgtgtg atcctgccct cactctggaa cagttcagaa
      481 tcctctgttt tcctctattt cataaaattt actgattttt aacatgggac agagagaccc
      541 aagggaagta gggtccggaa ggcttctggg atccgttgac ctgcaggtcg acctcgaggg
      601 ggggcccggt accagctttt gttcccttta gtgagggtta atttcgagct tggcgtaatc
      661 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
      721 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
      781 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
      841 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
      901 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
      961 ggtaatacgg ttatc
//
