LOCUS       sequence-17228-e        1003 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Knudson CM, Tung KS, Tourtellotte WG, Brown GA, Korsmeyer SJ
  TITLE     Bax-deficient mice with lymphoid hyperplasia and male germ cell 
            death.
  JOURNAL   Science 1995 Oct 6;270(5233):96-9.
  PUBMED    7569956
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science"; 
            date: "1995-10-6"; volume: "270(5233)"; pages: "96-9"
COMMENT     Sequence Label: 3185 pBS Bax cDNA
FEATURES             Location/Qualifiers
     source          1..1003
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(781..797)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(781..797)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(834..852)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(873..889)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(873..889)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(886..908)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    897..913
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    963..984
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cgcctccccg gccaccattc ccacccctcc caataattac aaaagtaaga aaaatgcctt
       61 tccccttccc ccattcatcc caggaaaatg tcataattta tgaagaaaag acacagtcca
      121 aggcagtggg aggcctcagc ccatcttctt ccagatggtg agcgaggcgg tgaggactcc
      181 agccacaaag atggtcactg tctgccatgt gggggtcccg aagtaggaga ggaggccttc
      241 ccagccaccc tggtcttgga tccagacaag cagccgctca cggaggaagt ccagtgtcca
      301 gcccatgatg gttctgatca gctcgggcac tttagtgcac agggccttga gcaccagttt
      361 gctagcaaag tagaagaggg caaccacgcg gccccagttg aagttgccat cagcaaacat
      421 gtcagctgcc acccggaaga agacctctcg gggggagtcc gtgtccacgt cagcaatcat
      481 cctctgcagc tccatattgc tgtccagttc atctccaatt cgccggagac actcgctcag
      541 cttcttggtg gacgcatcct ggggcggctg ctccaaggtc agctcaggtg tctccccagc
      601 catcctccct gctcgatcct ggatgaaacc ctgtagcaaa aaggcccctg tcttcatgat
      661 ctgttcagag ctggtgggcc cgccgctccc aagctgctcc ccggacccgt ccatcactgc
      721 cgctgcctct cgccgggtgc gccccggcgg ctgcggcgca ggaattcctg cagcccgggg
      781 gatccactag ttctagagcg gccgccaccg cggtggagct ccagcttttg ttccctttag
      841 tgagggttaa tttcgagctt ggcgtantca tggtcatagc tgtttcctgt gtgaaattgt
      901 tatccgctca caattcacac aacatacgag ccggaagcat aagtgtaaag cctggggtgc
      961 ctatgagtga gctaactcac attaatgcgt tgcgctcact gcc
//
