LOCUS       sequence-17234-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Harada H, Andersen JS, Mann M, Terada N, Korsmeyer SJ
  TITLE     p70S6 kinase signals cell survival as well as growth, inactivating 
            the pro-apoptotic molecule BAD.
  JOURNAL   Proc Natl Acad Sci U S A 2001 Aug 14;98(17):9666-70. Epub 2001 Aug 
            7.
  PUBMED    11493700
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A"; date: "2001-08-14"; volume: "98(17)"; pages: 
            "9666-70. Epub 2001 Aug 7"
COMMENT     Sequence Label: 3217 pGEM-4Z Bad S112A
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      668..677
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(698..716)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(699..716)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(724..741)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(724..740)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gnnnncgact ctagagganc cccgggtacc gagctcgaat tcggctatcc cggggctcac
       61 tgggaggggg tggagcctcc tttgcccaag tttcgatccc accaggactg gataatgcgc
      121 gtccagccgg cgctttgtcg catctgtgtt gcagtgcctg cgctctttgg gcgaggaagt
      181 cccttgaagg aaccctcaaa ctcatcgctc atccttcgga gctcacggcc gtagcgctgc
      241 gctgcccaga gattgggggg agccgaacgc gagcgtcctc gaaaagggct aagctcctcc
      301 tccatccctt catcctcctc ggtccccgct gggtacgcac tgtggcgact ccgagtctcc
      361 atagcccctg cgcctccatg atgactgttg gtggctgccc gtccctgctg atgaatgttg
      421 ctccccagga gacctggggc caggtaggga cctggctggt cctcagtgag gctagggccc
      481 aggcccctat ctgtagcact agcgtcttcc tgctcactcg gctcaaactc tgggatctgg
      541 aacatactct gggctgctgg tctccaccgc cttcctcccg cgtcgctccc caggctccgg
      601 attccgggat cggacttcct caagcctagg gcgtgtgcag gagccagcga gggctgcttt
      661 ggggttccca tggtggcggc ttaagccgaa ttcgtattct atagtgtcac ctaaatccaa
      721 ttcactggcc gtcgttttac aacgtcnnga ctgnnaaacc cnggngttac ccanttnatc
      781 nnnngcagca nntccccctt tcgccagctg nnntantnnn nnaannnnnn c
//
