LOCUS       sequence-172439-        6989 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-terminal part of the split-BioID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6989)
  AUTHORS   Munter S, Gornemann J, Derua R, Lesage B, Qian J, Heroes E, Waelkens
            E, Eynde AV, Beullens M, Bollen M
  TITLE     Split-BioID: a proximity-biotinylation assay for 
            dimerization-dependent protein interactions.
  JOURNAL   FEBS Lett. 2016 Dec 29. doi: 10.1002/1873-3468.12548.
  PUBMED    28032891
REFERENCE   2  (bases 1 to 6989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2016 Dec 29. doi: 10.1002/1873-3468.12548."
COMMENT     Sequence Label: pcDNA3.1 - BirA* (AA141-321) - HA - NIPP1
FEATURES             Location/Qualifiers
     source          1..6989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      167..302
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     229..248
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             383..1177
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(437..456)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1047..1066
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1351..1472
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1388..1407)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1442..1461
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1521..1537)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1521..1537)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1534..1556)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1545..1561
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1569..1575,1576..1593,1594..1599))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1614..1635
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1752..1769)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1923..2511)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2003..2022)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2682..3473,3474..3542))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3305..3324
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3543..3647)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3722..3741)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3913..4292
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4293..4496
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4447..4467
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4541..4560
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4541..4559
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5133..5159
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5139..5165
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     primer_bind     complement(6258..6275)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6264..6488
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6534..6962
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6621..6640)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6831..6852
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(join(6971..6989,1..2))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(6976..6989,1..316)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
       61 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
      121 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
      181 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
      241 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
      301 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga
      361 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
      421 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
      481 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
      541 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
      601 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
      661 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
      721 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
      781 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
      841 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
      901 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
      961 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     1021 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     1081 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     1141 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     1201 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     1261 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     1321 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     1381 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     1441 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     1501 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     1561 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     1621 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     1681 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     1741 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     1801 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     1861 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     1921 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     1981 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2041 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     2101 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     2161 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2221 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     2281 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     2341 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     2401 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     2461 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     2521 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     2581 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     2641 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     2701 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     2761 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     2821 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     2881 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     2941 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     3001 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     3061 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     3121 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     3181 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     3241 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     3301 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     3361 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     3421 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     3481 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     3541 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     3601 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     3661 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca
     3721 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg
     3781 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
     3841 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca
     3901 gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
     3961 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     4021 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     4081 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     4141 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     4201 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     4261 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     4321 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     4381 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     4441 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctct
     4501 ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact cactataggg
     4561 agacccaagc tggctagcgc ttaatgcagg gacctgctgc tgccatcgga ctgagtctgg
     4621 tgatcggaat cgtgatggcc gaggtgctga gaaagctggg agccgacaag gtgagagtga
     4681 agtggcctaa tgacctgtac ctccaggacc gcaagctggc tggcatcctg gtggagctga
     4741 caggcaagac aggcgatgcc gctcagatcg tgatcggagc cggaatcaac atggccatga
     4801 gaagagtgga ggagagcgtg gtgaaccagg gctggatcac cctgcaggag gctggcatca
     4861 acctggaccg gaacaccctg gccgccatgc tgatcagaga gctgagagcc gctctggagc
     4921 tgttcgagca ggagggactg gctccttacc tgagcagatg ggagaagctg gacaacttca
     4981 tcaacagacc tgtgaagctg atcatcggcg acaaggaaat cttcggcatc tccagaggaa
     5041 tcgacaagca gggagctctg ctgctggagc aggacggaat catcaagccc tggatgggcg
     5101 gagaaatctc cctgagaagc gcagagaagg cttaccctta cgatgtaccg gattacgcaa
     5161 gcttgatggc ggcagccgcg aactccggct ccagcctccc gctgttcgac tgcccgacct
     5221 gggcaggtaa accccctcct ggtttacatc tggatgtagt caaaggagac aagctaattg
     5281 agaaactgat tatcgatgag aagaagtatt acttatttgg gcgaaaccct gatttgtgcg
     5341 acttcacaat tgaccaccag tcttgctctc gggtccatgc tgccttggtc taccacaagc
     5401 atctgaagag agttttcctt atagatctca acagtacaca cggcactttc ttgggtcaca
     5461 ttcggttgga gcctcacaag cctcagcaaa ttcccatcga ctccacggtc tcatttggtg
     5521 catccacacg ggcatacact ctccgtgaga agcctcagac attgccatca gctgtgaaag
     5581 gagatgagaa gatgggtgga gaggatgatg aactcaaggg cttactgggg cttcccgagg
     5641 aggagacaga acttgataac ctgacagaat tcaacactgc ccacaacaag cggatttcca
     5701 ctctcaccat tgaggagggg aatctggaca ttcagagacc aaagaggaag aggaagaact
     5761 cacgggtgac ctttagtgag gatgatgaga tcatcaaccc agaggatatg gatccctcgg
     5821 ttggtcgctt tcggaacatg gtgcagactg cagtggtccc agtcaagaag aagcgggtgg
     5881 agggccctgg ctccctggtc ctggaggaat ccgggagcag gcgcatgcag aactttgcct
     5941 tcagtggagg actctacggg ggcctgcccc ccacgcacag tgaagctggc tcccagccac
     6001 acggcatcca tgggacagca ctcattggtg gcttgcccat gccatacccg aaccttgctc
     6061 ctgatgtgga cttgacccct gttgtgccat cagcagtgaa catgaaccct gcaccaaacc
     6121 ctgccgtcta taaccccgaa gctgtcaacg aacccaagaa gaagaaatac gcaaaagagg
     6181 cgtggccggg caagaagccc acaccttcct tactgatttg aaagcttagg cggccttaag
     6241 tttaaaccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     6301 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     6361 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     6421 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     6481 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     6541 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     6601 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     6661 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     6721 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     6781 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     6841 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     6901 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     6961 ttaattctgt ggaatgtgtg tcagttagg
//
