LOCUS       sequence-172452-        3294 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutively expresses LasR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Scott SR, Hasty J
  TITLE     Quorum Sensing Communication Modules for Microbial Consortia.
  JOURNAL   ACS Synth Biol. 2016 Sep 16;5(9):969-77. doi: 
            10.1021/acssynbio.5b00286. Epub 2016 May 19.
  PUBMED    27172092
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00286"; journalName: "ACS Synth Biol"; date: 
            "2016-09-16- 16"; volume: "5"; issue: "9"; pages: "969-77"
COMMENT     Sequence Label: Bsrs078-LasR
FEATURES             Location/Qualifiers
     source          1..3294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    10..109
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(370..375,376..392,393..399)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     terminator      1244..1315
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1331..1358
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(1391..1408)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(1470..1569)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1586..1605)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1587..1605)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1610..1626)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1610..1626)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2057..2848
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2877..3233
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     primer_bind     3271..3288
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3272..3288
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctcgggcccc aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt
       61 gatgagcaat gcttttttat aatgccaact ttgtacaaga aagctgggtc gaattggtat
      121 cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatc tgaagtggaa
      181 ttcatgagat ctgaattcaa aagatcttaa gtaagtaaga gtatacgtat atcggctaat
      241 aacgtattaa ggcgcttcgg cgcctttttt tatgggggta ttttcatccc aatccacacg
      301 tccaacgcac agcaaacacc acgtcgaccc tatcagctgc gtgctttcta tgagtcgttg
      361 ctgcataact tgacaattaa tcatccggct cgtataatgt gtggaagggc ccaagttcac
      421 ttaaaaagga gatcaacaat gaaagcaatt ttcgtactga aacatcttaa tcatgctggg
      481 gagggtttct aatggccttg gttgacggtt ttcttgagct ggaacgctca agtggaaaat
      541 tggagtggag cgccatcctg cagaagatgg cgagcgacct tggattctcg aagatcctgt
      601 tcggcctgtt gcctaaggac agccaggact acgagaacgc cttcatcgtc ggcaactacc
      661 cggccgcctg gcgcgagcat tacgaccggg ctggctacgc gcgggtcgac ccgacggtca
      721 gtcactgtac ccagagcgta ctgccgattt tctgggaacc gtccatctac cagacgcgaa
      781 agcagcacga gttcttcgag gaagcctcgg ccgccggcct ggtgtatggg ctgaccatgc
      841 cgctgcatgg tgctcgcggc gaactcggcg cgctgagcct cagcgtggaa gcggaaaacc
      901 gggccgaggc caaccgtttc atggagtcgg tcctgccgac cctgtggatg ctcaaggact
      961 acgcactgca gagcggtgcc ggactggcct tcgaacatcc ggtcagcaaa ccggtggttc
     1021 tgaccagccg ggagaaggaa gtgttgcagt ggtgcgccat cggcaagacc agttgggaga
     1081 tatcggttat ctgcaactgc tcggaagcca atgtgaactt ccatatggga aatattcggc
     1141 ggaagttcgg tgtgacctcc cgccgcgtag cggccattat ggccgttaat ttgggtctta
     1201 ttactctctg aggatccaaa ctcgagtaag gatctccagg catcaaataa aacgaaaggc
     1261 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     1321 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tataggatcc taactcgctc
     1381 ctcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     1441 cagctcactc aaaggcggta atcaattcga cccagctttc ttgtacaaag ttggcattat
     1501 aaaaaataat tgctcatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
     1561 cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt
     1621 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
     1681 tatatcatca tgcctcctct agaccagcca ggacagaaat gcctcgactt cgctgctgcc
     1741 caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc agtggacata
     1801 agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag
     1861 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
     1921 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
     1981 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
     2041 aacaaagtta aacatcatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga
     2101 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
     2161 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac
     2221 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
     2281 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
     2341 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
     2401 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
     2461 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
     2521 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
     2581 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
     2641 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg
     2701 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga
     2761 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg
     2821 cgagatcacc aaggtagtcg gcaaataacc ctcgagagca gttcaacctg ttgatagtac
     2881 gtactaagct ctcatgtttc acgtactaag ctctcatgtt taacgtacta agctctcatg
     2941 tttaacgaac taaaccctca tggctaacgt actaagctct catggctaac gtactaagct
     3001 ctcatgtttc acgtactaag ctctcatgtt tgaacaataa aattaatata aatcagcaac
     3061 ttaaatagcc tctaaggttt taagttttat aagaaaaaaa agaatatata aggcttttaa
     3121 agcttttaag gtttaacggt tgtggacaac aagccaggga tgtaacgcac tgagaagccc
     3181 ttagagcctc tcaaagcaat tttgagtgac acaggaacac ttaacggctg acatgggaat
     3241 tagccatggg cccgtgcgaa tcaccctagg tgtaaaacga cggccagtct taag
//
