LOCUS       sequence-172453-        3307 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutively expresses RpaR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Scott SR, Hasty J
  TITLE     Quorum Sensing Communication Modules for Microbial Consortia.
  JOURNAL   ACS Synth Biol. 2016 Sep 16;5(9):969-77. doi: 
            10.1021/acssynbio.5b00286. Epub 2016 May 19.
  PUBMED    27172092
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00286"; journalName: "ACS Synth Biol"; date: 
            "2016-09-16- 16"; volume: "5"; issue: "9"; pages: "969-77"
COMMENT     Sequence Label: Bsrs078-RpaR
FEATURES             Location/Qualifiers
     source          1..3307
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    10..109
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(371..376,377..393,394..400)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     terminator      1257..1328
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1344..1371
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(1404..1421)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(1483..1582)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1599..1618)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1600..1618)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1623..1639)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1623..1639)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2070..2861
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2890..3246
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     primer_bind     3284..3301
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3285..3301
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctcgggcccc aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt
       61 gatgagcaat gcttttttat aatgccaact ttgtacaaaa aagcaggctc cgaattggta
      121 tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat ctgaagtgga
      181 attcatgaga tctgaattca aaagatctta agtaagtaag agtatacgta tatcggctaa
      241 taacgtatta aggcgcttcg gcgccttttt ttatgggggt attttcatcc caatccacac
      301 gtccaacgca cagcaaacac cacgtcgacc ctatcagctg cgtgctttct atgagtcgtt
      361 gctgcataac ttgacaatta atcatccggc tcgtataatg tgtggaaggg cccaagttca
      421 cttaaaaagg agatcaacaa tgaaagcaat tttcgtactg aaacatctta atcatgcgat
      481 ggacggtttc taatgatcgt cggcgaagat cagctttggg gacggcgtgc gctggaattc
      541 gtcgattccg tcgaacggct cgaggcgccg gcgctgatca gccggttcga atcgctgatc
      601 gcgagctgcg gatttaccgc ctacatcatg gccggcctgc cgtcgcgcaa tgccggacta
      661 ccggagctga cgctggccaa tggctggccg cgagactggt tcgatctgta tgtcagcgaa
      721 aacttcagcg cggtcgatcc ggtgccgcgc cacggcgcta ccacggttca tcctttcgta
      781 tggtccgatg caccctacga ccgcgaccgt gatccggccg cccaccgggt catgacccgg
      841 gcggcggaat tcggactggt cgagggttac tgcattccgc tgcactacga cgacggtagc
      901 gccgcgatca gcatggccgg caaggatccg gacctcagcc cggccgcgcg cggcgcgatg
      961 cagctggtca gcatctacgc gcatagtcgc ctgcgcgcac tcagccggcc aaagccgatc
     1021 cggcgcaacc ggctcacgcc gcgcgagtgc gagatcctgc aatgggcagc gcagggcaag
     1081 accgcctggg aaatctcggt aatcctctgc atcaccgaac gcacggtgaa attccatctg
     1141 atcgaagccg cccgcaagct cgacgccgcc aaccgcaccg cggcggttgc caaggcattg
     1201 acgctcggat tgatccgttt gtgaggatcc aaactcgagt aaggatctcc aggcatcaaa
     1261 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     1321 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatagga
     1381 tcctaactcg ctcctcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     1441 cggcgagcgg tatcagctca ctcaaaggcg gtaatcaatt cgacccagct ttcttgtaca
     1501 aagttggcat tataaaaaat aattgctcat caatttgttg caacgaacag gtcactatca
     1561 gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca
     1621 tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg
     1681 cacaagataa aaatatatca tcatgcctcc tctagaccag ccaggacaga aatgcctcga
     1741 cttcgctgct gcccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa
     1801 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
     1861 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
     1921 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
     1981 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
     2041 gcagtcgccc taaaacaaag ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga
     2101 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     2161 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     2221 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     2281 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     2341 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     2401 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     2461 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     2521 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     2581 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     2641 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     2701 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     2761 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     2821 actacgtgaa aggcgagatc accaaggtag tcggcaaata accctcgaga gcagttcaac
     2881 ctgttgatag tacgtactaa gctctcatgt ttcacgtact aagctctcat gtttaacgta
     2941 ctaagctctc atgtttaacg aactaaaccc tcatggctaa cgtactaagc tctcatggct
     3001 aacgtactaa gctctcatgt ttcacgtact aagctctcat gtttgaacaa taaaattaat
     3061 ataaatcagc aacttaaata gcctctaagg ttttaagttt tataagaaaa aaaagaatat
     3121 ataaggcttt taaagctttt aaggtttaac ggttgtggac aacaagccag ggatgtaacg
     3181 cactgagaag cccttagagc ctctcaaagc aattttgagt gacacaggaa cacttaacgg
     3241 ctgacatggg aattagccat gggcccgtgc gaatcaccct aggtgtaaaa cgacggccag
     3301 tcttaag
//
