LOCUS       sequence-172454-        3279 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutively expresses TraR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Scott SR, Hasty J
  TITLE     Quorum Sensing Communication Modules for Microbial Consortia.
  JOURNAL   ACS Synth Biol. 2016 Sep 16;5(9):969-77. doi: 
            10.1021/acssynbio.5b00286. Epub 2016 May 19.
  PUBMED    27172092
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00286"; journalName: "ACS Synth Biol"; date: 
            "2016-09-16- 16"; volume: "5"; issue: "9"; pages: "969-77"
COMMENT     Sequence Label: Bsrs078-TraR
FEATURES             Location/Qualifiers
     source          1..3279
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    10..109
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(370..375,376..392,393..399)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     CDS             773..784
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      1229..1300
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1316..1343
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(1376..1393)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(1455..1554)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1571..1590)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1572..1590)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1595..1611)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1595..1611)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2042..2833
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2862..3218
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     primer_bind     3256..3273
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3257..3273
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctcgggcccc aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt
       61 gatgagcaat gcttttttat aatgccaact ttgtacaaaa aagcaggctc cgaattggta
      121 tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat ctgaagtgga
      181 attcatgaga tctgaattca aaagatctta agtaagtaag agtatacgta tatcggctaa
      241 taacgtatta aggcgcttcg gcgccttttt tatgggggta ttttcatccc aatccacacg
      301 tccaacgcac agcaaacacc acgtcgaccc tatcagctgc gtgctttcta tgagtcgttg
      361 ctgcataact tgacaattaa tcatccggct cgtataatgt gtggaagggc ccaagttcac
      421 ttaaaaagga gatcaacaat gaaagcaatt ttcgtactga aacatcttaa tcatgctggg
      481 gagggtttct aatgcagcac tggctggaca agttgaccga tcttgccgca attcagggcg
      541 acgagtgcat cctgaaggat ggccttgccg accttgccga acatttcggc ttcaccggct
      601 atgcctatct ccatatccag cacaaacaca ccatcgcggt caccaattat catcgtgact
      661 ggcgatcggc ttacttcgag aacaacttcg acaagctcga tccggtcgtc aagcgcgcga
      721 aatccaggaa gcacgtcttt gcctggtccg gcgaacagga acgatcgcgg ctatcgaagg
      781 aagagcgtgc cttctacgcg catgcggccg atttcggcat ccgctccggc atcaccattc
      841 cgatcaagac cgccaacgga tcaatgtcga tgttcacgct ggcgtcggaa aggccggcga
      901 tcgacctcga ccgtgagatc gacgcggccg cagccgcggg cgccgtcggg cagctccatg
      961 cccgcatctc tttccttcag accactccga cagtggaaga tgccgcctgg ctcgatccga
     1021 aagaggcgac ctatctcaga tggatcgccg tcggcatgac aatggaggaa gtcgcagacg
     1081 tggagggcgt caagtacaac agcgtccgtg tcaagctccg cgaggccatg aagcgcttcg
     1141 acgttcgcag caaggcccat ctcaccgccc tcgcaatcag aagaaagctg atctaaggat
     1201 ccaaactcga gtaaggatct ccaggcatca aataaaacga aaggctcagt cgaaagactg
     1261 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     1321 accttcgggt gggcctttct gcgtttatag gatcctaact cgctcctcag gcttcctcgc
     1381 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     1441 cggtaatcaa ttcgacccag ctttcttgta caaagttggc attataaaaa ataattgctc
     1501 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc
     1561 agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg
     1621 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgcct
     1681 cctctagacc agccaggaca gaaatgcctc gacttcgctg ctgcccaagg ttgccgggtg
     1741 acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct gttcggttcg
     1801 taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
     1861 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt
     1921 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
     1981 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat
     2041 catgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
     2101 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
     2161 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga
     2221 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
     2281 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
     2341 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
     2401 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
     2461 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
     2521 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
     2581 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
     2641 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
     2701 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc
     2761 ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt
     2821 agtcggcaaa taaccctcga gagcagttca acctgttgat agtacgtact aagctctcat
     2881 gtttcacgta ctaagctctc atgtttaacg tactaagctc tcatgtttaa cgaactaaac
     2941 cctcatggct aacgtactaa gctctcatgg ctaacgtact aagctctcat gtttcacgta
     3001 ctaagctctc atgtttgaac aataaaatta atataaatca gcaacttaaa tagcctctaa
     3061 ggttttaagt tttataagaa aaaaaagaat atataaggct tttaaagctt ttaaggttta
     3121 acggttgtgg acaacaagcc agggatgtaa cgcactgaga agcccttaga gcctctcaaa
     3181 gcaattttga gtgacacagg aacacttaac ggctgacatg ggaattagcc atgggcccgt
     3241 gcgaatcacc ctaggtgtaa aacgacggcc agtcttaag
//
