LOCUS       sequence-172455-        2918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducibile Tra* Promoter driving sfGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Scott SR, Hasty J
  TITLE     Quorum Sensing Communication Modules for Microbial Consortia.
  JOURNAL   ACS Synth Biol. 2016 Sep 16;5(9):969-77. doi: 
            10.1021/acssynbio.5b00286. Epub 2016 May 19.
  PUBMED    27172092
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00286"; journalName: "ACS Synth Biol"; date: 
            "2016-09-16- 16"; volume: "5"; issue: "9"; pages: "969-77"
COMMENT     Sequence Label: Bsrs074-Ptra*
FEATURES             Location/Qualifiers
     source          1..2918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    10..109
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             247..255
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             262..975
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1005..1022)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(1084..1183)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1200..1219)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1201..1219)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1224..1240)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1224..1240)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1353..1457
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1458..1526,1527..2318)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1676..1695)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     2895..2912
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2896..2912
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctcgggcccc aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt
       61 gatgagcaat gcttttttat aatgccaact ttgtacaaaa aagcaggctc cgaattggta
      121 tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat ctgaagtgga
      181 attcatgaga tctgcacgtg cagatctgca catttacgca agaaaatggt ttgttatagt
      241 cgaatataag gaggtgacaa tatgcgtaaa ggcgaagagc tgttcactgg tgtcgtccct
      301 attctggtgg aactggatgg tgatgtcaac ggtcataagt tttccgtgcg tggcgagggt
      361 gaaggtgacg caactaatgg taaactgacg ctgaagttca tctgtactac tggtaaactg
      421 ccggtacctt ggccgactct ggtaacgacg ctgacttatg gtgttcagtg ctttgctcgt
      481 tatccggacc atatgaagca gcatgacttc ttcaagtccg ccatgccgga aggctatgtg
      541 caggaacgca cgatttcctt taaggatgac ggcacgtaca aaacgcgtgc ggaagtgaaa
      601 tttgaaggcg ataccctggt aaaccgcatt gagctgaaag gcattgactt taaagaagac
      661 ggcaatatcc tgggccataa gctggaatac aattttaaca gccacaatgt ttacatcacc
      721 gccgataaac aaaaaaatgg cattaaagcg aattttaaaa ttcgccacaa cgtggaggat
      781 ggcagcgtgc agctggctga tcactaccag caaaacactc caatcggtga tggtcctgtt
      841 ctgctgccag acaatcacta tctgagcacg caaagcgttc tgtctaaaga tccgaacgag
      901 aaacgcgatc atatggttct gctggagttc gtaaccgcag cgggcatcac gcatggtatg
      961 gatgaactgt acaaatgagg atcctaactc gctcctcagg cttcctcgct cactgactcg
     1021 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatcaat
     1081 tcgacccagc tttcttgtac aaagttggca ttataaaaaa taattgctca tcaatttgtt
     1141 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc
     1201 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc
     1261 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagactt
     1321 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     1381 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     1441 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     1501 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     1561 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     1621 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     1681 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     1741 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     1801 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     1861 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     1921 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     1981 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     2041 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     2101 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     2161 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     2221 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     2281 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     2341 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     2401 tttttgataa tcctcgagga gcgcctcagc gcgccgtagc gtcgataaaa attacgggct
     2461 ggggcgaaac taccatctgt tcgaaaaggt ccgtaaatgg gcctacagag cgattcgtca
     2521 gggctggcct gtattctcac aatggcttga tgccgttatc cagcgtgtcg aaatgtacaa
     2581 cgcttcgctt cccgttccgc tttctccggc tgaatgtcgg gctattggca agagcattgc
     2641 gaaatataca cacaggaaat tctcaccaga gggattttcc gctgtacagg ccgctcgcgg
     2701 tcgcaagggc ggaactaaat ctaagcgcgc agcagttcct acatcagcac gttcgctgaa
     2761 accgtgggag gcattaggca tcagtcgagc gacgtactac cgaaaattaa aatgtgaccc
     2821 agacctcgca aaatgagacc agataagcct tatcagataa cagcgccctt ttggcgtctt
     2881 tttgagcacc taggtgtaaa acgacggcca gtcttaag
//
