LOCUS       sequence-172456-        3350 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducibile Las Promoter driving sfGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Scott SR, Hasty J
  TITLE     Quorum Sensing Communication Modules for Microbial Consortia.
  JOURNAL   ACS Synth Biol. 2016 Sep 16;5(9):969-77. doi: 
            10.1021/acssynbio.5b00286. Epub 2016 May 19.
  PUBMED    27172092
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00286"; journalName: "ACS Synth Biol"; date: 
            "2016-09-16- 16"; volume: "5"; issue: "9"; pages: "969-77"
COMMENT     Sequence Label: Bsrs074-Plas
FEATURES             Location/Qualifiers
     source          1..3350
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    10..109
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             694..1407
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1437..1454)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(1516..1615)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1632..1651)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1633..1651)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1656..1672)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1656..1672)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1785..1889
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1890..1958,1959..2750)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2108..2127)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     3327..3344
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3328..3344
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctcgggcccc aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt
       61 gatgagcaat gcttttttat aatgccaact ttgtacaaaa aagcaggctc cgaattggta
      121 tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat ctgaagtgga
      181 attcatgaga tctttcgagc ctagcaaggg tccgggttca ccgaaatcta tctcatttgc
      241 tagttataaa attatgaaat ttgcgtaaat tcttcaaagg aggtgacaat atgcgtaaag
      301 gcgaagaggc tccgaattgg tatcacgagg cagaatttca gataaaaaaa atccttagct
      361 ttcgctaagg atctgaagtg gaattcatga gactttcgag cctagcaagg gtccgggttc
      421 accgaaatct atctcatttg ctagttataa aattatgaaa tttgcgtaaa ttcttcaaag
      481 gaggtgacaa tatgcgtaaa ggcgaagagg ctccgaattg gtatcacgag gcagaatttc
      541 agataaaaaa aatccttagc tttcgctaag gatctgaagt ggaattcatg agatctttcg
      601 agcctagcaa gggtccgggt tcaccgaaat ctatctcatt tgctagttat aaaattatga
      661 aatttgcgta aattcttcaa aggaggtgac aatatgcgta aaggcgaaga gctgttcact
      721 ggtgtcgtcc ctattctggt ggaactggat ggtgatgtca acggtcataa gttttccgtg
      781 cgtggcgagg gtgaaggtga cgcaactaat ggtaaactga cgctgaagtt catctgtact
      841 actggtaaac tgccggtacc ttggccgact ctggtaacga cgctgactta tggtgttcag
      901 tgctttgctc gttatccgga ccatatgaag cagcatgact tcttcaagtc cgccatgccg
      961 gaaggctatg tgcaggaacg cacgatttcc tttaaggatg acggcacgta caaaacgcgt
     1021 gcggaagtga aatttgaagg cgataccctg gtaaaccgca ttgagctgaa aggcattgac
     1081 tttaaagaag acggcaatat cctgggccat aagctggaat acaattttaa cagccacaat
     1141 gtttacatca ccgccgataa acaaaaaaat ggcattaaag cgaattttaa aattcgccac
     1201 aacgtggagg atggcagcgt gcagctggct gatcactacc agcaaaacac tccaatcggt
     1261 gatggtcctg ttctgctgcc agacaatcac tatctgagca cgcaaagcgt tctgtctaaa
     1321 gatccgaacg agaaacgcga tcatatggtt ctgctggagt tcgtaaccgc agcgggcatc
     1381 acgcatggta tggatgaact gtacaaatga ggatcctaac tcgctcctca ggcttcctcg
     1441 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     1501 gcggtaatca attcgaccca gctttcttgt acaaagttgg cattataaaa aataattgct
     1561 catcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc
     1621 cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct
     1681 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgcc
     1741 tcctctagac ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     1801 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     1861 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     1921 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     1981 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     2041 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     2101 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     2161 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     2221 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     2281 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     2341 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     2401 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     2461 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     2521 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     2581 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     2641 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     2701 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     2761 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     2821 aggtgaagat cctttttgat aatcctcgag gagcgcctca gcgcgccgta gcgtcgataa
     2881 aaattacggg ctggggcgaa actaccatct gttcgaaaag gtccgtaaat gggcctacag
     2941 agcgattcgt cagggctggc ctgtattctc acaatggctt gatgccgtta tccagcgtgt
     3001 cgaaatgtac aacgcttcgc ttcccgttcc gctttctccg gctgaatgtc gggctattgg
     3061 caagagcatt gcgaaatata cacacaggaa attctcacca gagggatttt ccgctgtaca
     3121 ggccgctcgc ggtcgcaagg gcggaactaa atctaagcgc gcagcagttc ctacatcagc
     3181 acgttcgctg aaaccgtggg aggcattagg catcagtcga gcgacgtact accgaaaatt
     3241 aaaatgtgac ccagacctcg caaaatgaga ccagataagc cttatcagat aacagcgccc
     3301 ttttggcgtc tttttgagca cctaggtgta aaacgacggc cagtcttaag
//
