LOCUS       sequence-172457-        2920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducibile Rpa* Promoter driving sfGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Scott SR, Hasty J
  TITLE     Quorum Sensing Communication Modules for Microbial Consortia.
  JOURNAL   ACS Synth Biol. 2016 Sep 16;5(9):969-77. doi: 
            10.1021/acssynbio.5b00286. Epub 2016 May 19.
  PUBMED    27172092
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00286"; journalName: "ACS Synth Biol"; date: 
            "2016-09-16- 16"; volume: "5"; issue: "9"; pages: "969-77"
COMMENT     Sequence Label: Bsrs074-Prpa*
FEATURES             Location/Qualifiers
     source          1..2920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    10..109
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             249..257
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             264..977
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1007..1024)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(1086..1185)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1202..1221)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1203..1221)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1226..1242)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1226..1242)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1355..1459
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1460..1528,1529..2320)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1678..1697)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     2897..2914
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2898..2914
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctcgggcccc aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt
       61 gatgagcaat gcttttttat aatgccaact ttgtacaaaa aagcaggctc cgaattggta
      121 tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat ctgaagtgga
      181 attcatgaga tctgcacctg tccgatcgga cagtattacg caagaaaatg gtttgttata
      241 gtcgaatata aggaggtgac aatatgcgta aaggcgaaga gctgttcact ggtgtcgtcc
      301 ctattctggt ggaactggat ggtgatgtca acggtcataa gttttccgtg cgtggcgagg
      361 gtgaaggtga cgcaactaat ggtaaactga cgctgaagtt catctgtact actggtaaac
      421 tgccggtacc ttggccgact ctggtaacga cgctgactta tggtgttcag tgctttgctc
      481 gttatccgga ccatatgaag cagcatgact tcttcaagtc cgccatgccg gaaggctatg
      541 tgcaggaacg cacgatttcc tttaaggatg acggcacgta caaaacgcgt gcggaagtga
      601 aatttgaagg cgataccctg gtaaaccgca ttgagctgaa aggcattgac tttaaagaag
      661 acggcaatat cctgggccat aagctggaat acaattttaa cagccacaat gtttacatca
      721 ccgccgataa acaaaaaaat ggcattaaag cgaattttaa aattcgccac aacgtggagg
      781 atggcagcgt gcagctggct gatcactacc agcaaaacac tccaatcggt gatggtcctg
      841 ttctgctgcc agacaatcac tatctgagca cgcaaagcgt tctgtctaaa gatccgaacg
      901 agaaacgcga tcatatggtt ctgctggagt tcgtaaccgc agcgggcatc acgcatggta
      961 tggatgaact gtacaaatga ggatcctaac tcgctcctca ggcttcctcg ctcactgact
     1021 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatca
     1081 attcgaccca gctttcttgt acaaagttgg cattataaaa aataattgct catcaatttg
     1141 ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat
     1201 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc
     1261 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgcc tcctctagac
     1321 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     1381 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     1441 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     1501 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     1561 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     1621 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     1681 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     1741 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     1801 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     1861 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     1921 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     1981 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     2041 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     2101 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     2161 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     2221 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     2281 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     2341 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     2401 cctttttgat aatcctcgag gagcgcctca gcgcgccgta gcgtcgataa aaattacggg
     2461 ctggggcgaa actaccatct gttcgaaaag gtccgtaaat gggcctacag agcgattcgt
     2521 cagggctggc ctgtattctc acaatggctt gatgccgtta tccagcgtgt cgaaatgtac
     2581 aacgcttcgc ttcccgttcc gctttctccg gctgaatgtc gggctattgg caagagcatt
     2641 gcgaaatata cacacaggaa attctcacca gagggatttt ccgctgtaca ggccgctcgc
     2701 ggtcgcaagg gcggaactaa atctaagcgc gcagcagttc ctacatcagc acgttcgctg
     2761 aaaccgtggg aggcattagg catcagtcga gcgacgtact accgaaaatt aaaatgtgac
     2821 ccagacctcg caaaatgaga ccagataagc cttatcagat aacagcgccc ttttggcgtc
     2881 tttttgagca cctaggtgta aaacgacggc cagtcttaag
//
