LOCUS       sequence-172475-        7719 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Flag-tagged FLJ10081 (NSL3).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7719)
  AUTHORS   Cai Y, Jin J, Swanson SK, Cole MD, Choi SH, Florens L, Washburn MP, 
            Conaway JW, Conaway RC
  TITLE     Subunit composition and substrate specificity of a MOF-containing 
            histone acetyltransferase distinct from the male-specific lethal 
            (MSL) complex.
  JOURNAL   J Biol Chem. 2010 Feb 12. 285(7):4268-72.
  PUBMED    20018852
REFERENCE   2  (bases 1 to 7719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2010 Feb 12. 285(7):4268-72."
COMMENT     Sequence Label: pcDNA5FRT/Flag-FLJ10081
FEATURES             Location/Qualifiers
     source          1..7719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             907..930
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(3671..3688)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3677..3901
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4034..4053)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4185..4232
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4240..5262
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4240..5262
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5392..5513
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5429..5448)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5483..5502
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5562..5578)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5562..5578)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5575..5597)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5586..5602
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5610..5616,5617..5634,5635..5640))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5655..5676
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5793..5810)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5964..6552)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6044..6063)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6723..7514,7515..7583))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7346..7365
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7584..7688)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 atgccagact acaaggacga tgatgacaag ctcgacatgg cccaccgggg tggggagagg
      961 gacttccaga cttcagctcg acgcatgggc acctcgctgc tcttccagct ttcagtgcat
     1021 gaacgggagc tggacctggt ttttctggat catagctatg ccaagccttg gagtgcccac
     1081 ccagatgcca gtagtgcccg ccccacccgc atgctctttg tcactccccg gcggcagcac
     1141 gaaagtacca ttgaatcaga cgtcccaata gatgtggaga cggtcacatc aacgcctatg
     1201 ccactctatg acaatcagaa ggcacgcagc gtgatgaatg agtgtgaacg gcatgtcatc
     1261 tttgccagga ctgatgcaga tgcccctcct ccaccagagg actgggagga gcatgtcaac
     1321 aggactggct ggacaatggc ccagaacaag ctattcaaca agatcctcaa agccctgcag
     1381 tctgaccggc ttgcccgctt ggccaacgaa ggggcttgta atgagccagt gctgcgccgt
     1441 gttgctgtgg acaagtgtgc aaggagagtg cggcaggctc tggcaagtgt gagctgggat
     1501 accaagctga tccagtggct gcacaccacc cttgtggaga ccttgagtct gcccatgctg
     1561 gcagcctacc tggatgcttt gcagacgctg aaggggaaga tcccaacctt gattgaccgg
     1621 atgcttgtgt catccaacac aaagactggg gctgcaggag ctgaggcctt gtctctccta
     1681 ctgaagaggc cctgggaccc tgctgtgggt gtgctttctc ataacaaacc aagcaaactc
     1741 cctggctctc cgctgattct catcgcctcc tctggtccct ccagctctgt gtttcccact
     1801 tcacgccgcc accgcttctg gcaatctcag ctgtcctgct tgggcaaggt catccctgta
     1861 gccacccatc tgctgaacaa tggcagtggg gtaggagttc tacagtgtct cgagcatatg
     1921 attggggcag tgagaagcaa agtgctggag attcacagcc atttcccaca caaacccatt
     1981 atcttgattg gctggaacac aggagctttg gtggcctgtc atgtgtcagt aatggagtat
     2041 gtcactgcag ttgtctgcct tgggtttcct ctgcttactg tggatggccc cagaggggat
     2101 gtagatgatc ccctcttgga tatgaagact ccagtcctct ttgtcattgg tcagaattcc
     2161 cttcaatgtc accctgaagc catggaggac ttccgggaga agattcgagc tgagaacagc
     2221 ttggtggtgg ttgggggagc tgatgacaat ctcagaataa gcaaagcaaa gaagaaatca
     2281 gaagggttga ctcagagcat ggtggacaga tgtattcagg atgagattgt ggactttctg
     2341 actggggtgc tcactcgtgc tgagggtcac atgggctctg aacctcggga tcaggatgct
     2401 gagaagaaga agaagccccg cgatgtggcc cgcagagact tggcctttga agtccctgag
     2461 cggggcagtc gacctgcctc cccagctgcc aagctgcccg cctcaccctc aggctcagag
     2521 gatctctcca gtgtgtccag cagccccacc tccagtccca agaccaaagt gaccacagtg
     2581 acctctgccc agaagtccag tcagattgga agttctcagc tgctgaagag acatgtgcag
     2641 cggacagaag ctgtgctgac ccacaaacaa gctcaagttc ccatttcatc agaaccacca
     2701 gaggaaggag agaaagagga tcttagggtt cagctgaagc gacaccatcc ctcgagtccc
     2761 cttcctggca gtaagacctc caaacgaccg aagatcaagg tgtcccttat ctcccaaggg
     2821 gacacagctg gagggccttg tgctccttcc caaggaagtg ctccagaagc tgcaggtggg
     2881 aagcccatca ccatgacact ggggcaggct tcagcagggg ccaaggagct cacaggactt
     2941 ctcaccacag ccaagtccag ttcttctgaa ggtggagtct cagccagccc agtcccttca
     3001 gtggtctcca gcagcactgc acccagtgcc ttgcacacac tgcagagccg cctggtggcc
     3061 acatctcctg gcagctccct cccaggggcc acatcagcca gcagcctcct ccaaggcctc
     3121 agcttcagct tgcaggatat cagcagcaag acctctggcc ttccagcaaa tccctcccca
     3181 ggaccagccc cacaggccac cagtgtgaag ttgcccaccc ccatgcagag cctgggtgcc
     3241 atcaccacgg gcaccagcac cattgtccgt accattcctg tggccaccac tctctcctcc
     3301 ttgggtgcca ctcctggtgg gaagcccaca gccatccacc agctgctgac caatgggggc
     3361 ctcgctaagt tggcaagcag cctccctggc ctggctcaga tctctaacca agcatcaggc
     3421 ttgaaggtcc ccaccaccat tactctgaca cttcgtggcc agccgagcag gatcactaca
     3481 ctgagcccta tgggctcagg agcagcccca tccgaggagt cctcttccca ggtgctgccc
     3541 tccagctcac agcgcctgcc tccagcaccc tgaggatcca ctagtccagt gtggtggaat
     3601 tctgcagata tccagcacag tggcggccgc tcgatcgagt ctagagggcc cgtttaaacc
     3661 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     3721 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     3781 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     3841 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     3901 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     3961 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     4021 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     4081 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     4141 ctcgacccca aaaaacttga ttagggtgat ggttcacgta cctagaagtt cctattccga
     4201 agttcctatt ctctagaaag tataggaact tccttggcca aaaagcctga actcaccgcg
     4261 acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc
     4321 tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg
     4381 cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca
     4441 tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc
     4501 tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg
     4561 cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc
     4621 cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt
     4681 gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac
     4741 accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc
     4801 cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat
     4861 ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag
     4921 gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac
     4981 ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc
     5041 attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg
     5101 gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa
     5161 atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt
     5221 ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat agcacgtact acgagatttc
     5281 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     5341 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     5401 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     5461 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     5521 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     5581 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     5641 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     5701 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     5761 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     5821 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     5881 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     5941 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     6001 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     6061 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     6121 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     6181 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     6241 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     6301 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     6361 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     6421 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     6481 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     6541 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     6601 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     6661 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     6721 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     6781 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     6841 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     6901 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     6961 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     7021 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     7081 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     7141 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     7201 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     7261 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     7321 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     7381 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     7441 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     7501 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     7561 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     7621 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     7681 gttccgcgca catttccccg aaaagtgcca cctgacgtc
//
