LOCUS       sequence-172476-       13718 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dox inducible expression of reprogramming factors (Klf4, Sox2, 
            Oct4,.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 13718)
  AUTHORS   Merkl C, Saalfrank A, Riesen N, Kuhn R, Pertek A, Eser S, Hardt MS, 
            Kind A, Saur D, Wurst W, Iglesias A, Schnieke A
  TITLE     Efficient generation of rat induced pluripotent stem cells using a 
            non-viral inducible vector.
  JOURNAL   PLoS One. 2013;8(1):e55170. doi: 10.1371/journal.pone.0055170. Epub 
            2013 Jan 31.
  PUBMED    23383095
REFERENCE   2  (bases 1 to 13718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013"; volume: "8(1):e55170. doi"; pages: " 
            10.1371/journal.pone.0055170. Epub 2013 Jan 31"
COMMENT     Sequence Label: pREPROII-attB
FEATURES             Location/Qualifiers
     source          1..13718
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..27
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             join(89..706,707..712,713..748,749..751,752..787,788..790,
                     791..826,827..835)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(1562..1581)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             2125..2145
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2248..2442
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     polyA_signal    2513..2647
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2537..2556)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2591..2610
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2921..2940)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     complement(3031..3051)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3031..3049)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3070..3086)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3070..3086)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3083..3105)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3094..3110
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3118..3124,3125..3142,3143..3148))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3163..3184
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3301..3318)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3472..4060)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3552..3571)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4231..5022,5023..5091))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4854..4873
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5092..5196)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5222..5677)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5359..5380)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5571..5590
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5804..5826
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5818..5835
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5819..5835
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5845..5863
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5930..5963)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    6015..6048
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    complement(6085..6118)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(6132..6150)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    6221..6445
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(7901..7954)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     intron          8997..9226
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     protein_bind    join(9393..9411,9412..9434,9435..9453,9454..9476,9477..9495,
                     9496..9518,9519..9537,9538..9560,9561..9579,9580..9602,
                     9603..9621,9622..9644,9645..9663)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        9696..9734
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          9873..10102
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     regulatory      10179..10188
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             11241..11294
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     polyA_signal    12722..12946
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        12998..13377
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     13642..13664
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcgtcgac
       61 gggatcctgg ccagctagcg aattcaccat gtctagactg gacaagagca aagtcataaa
      121 cggcgctctg gaattactca atggagtcgg tatcgaaggc ctgacgacaa ggaaactcgc
      181 tcaaaagctg ggagttgagc agcctaccct gtactggcac gtgaagaaca agcgggccct
      241 gctcgatgcc ctgccaatcg agatgctgga caggcatcat acccacttct gccccctgga
      301 aggcgagtca tggcaagact ttctgcggaa caacgccaag tcattccgct gtgctctcct
      361 ctcacatcgc gacggggcta aagtgcatct cggcacccgc ccaacagaga aacagtacga
      421 aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc ttctccctgg agaacgcact
      481 gtacgctctg tccgccgtgg gccactttac actgggctgc gtattggagg aacaggagca
      541 tcaagtagca aaagaggaaa gagagacacc taccaccgat tctatgcccc cacttctgag
      601 acaagcaatt gagctgttcg accggcaggg agccgaacct gccttccttt tcggcctgga
      661 actaatcata tgtggcctgg agaaacagct aaagtgcgaa agcggcgggc cggccgacgc
      721 ccttgacgat tttgacttag acatgctccc agccgatgcc cttgacgact ttgaccttga
      781 tatgctgcct gctgacgctc ttgacgattt tgaccttgac atgctccccg ggtaactatt
      841 aaaacagctc tggggttgta cccaccccag aggcccacgt ggcggctagt actccggtat
      901 tgcggtaccc ttgtacgcct gttttatact cccttcccgt aacttagacg cacaaaacca
      961 agttcaatag aagggggtac aaaccagtac caccacgaac aagcacttct gtttccccgg
     1021 tgatgtcgta tagactgctt gcgtggttga aagcgacgga tccgttatcc gcttatgtac
     1081 ttcgagaagc ccagtaccac ctcggaatct tcaatgcgtt gcgctcagca ctcaacccca
     1141 gagtgtagct taggctgatg agtctggaca tccctcaccg gtgacggtgg tccaggctgc
     1201 gttggcggcc tacctatggc taacgccatg ggacgctagt tgtgaacaag gtgtgaagag
     1261 cctattgagc tacataagaa tcctccggcc cctgaatgcg gctaatccca acctcggagc
     1321 aggtggtcac aaaccagtga ttggcctgtc gtaacgcgca agtccgtggc ggaaccgact
     1381 actttgggtg tccgtgtttc cttttatttt attgtggctg cttatggtga caatcacaga
     1441 ttgttatcat aaagcgaatt ggattcccgg gtaactaagt aaggatccat gtctagatta
     1501 gataaaagta aagtgattaa cagcgcatta gagctgctta atgaggtcgg aatcgaaggt
     1561 ttaacaaccc gtaaactcgc ccagaagctt ggtgtagagc agcctacatt gtattggcat
     1621 gtaaaaaata agcgggccct tatgaacatg ctttcagagg caatactggc gaagcatcac
     1681 acccgttcag caccgttacc gactgagagt tggcagcagt ttctccagga aaatgctctg
     1741 agtttccgta aagcattact ggtccatcgt gatggagccc gattgcatat agggacctct
     1801 cctacgcccc cccagtttga acaagcagag gcgcaactac gctgtctatg cgatgcaggg
     1861 ttttcggtcg aggaggctct tttcattctg caatctatca gccattttac gttgggtgca
     1921 gtattagagg agcaagcaac aaaccagata gaaaataatc atgtgataga cgctgcacca
     1981 ccattattac aagaggcatt taatattcag gcgagaacct ctgctgaaat ggccttccat
     2041 ttcgggctga aatcattaat atttggattt tctgcacagt tagatgaaaa aaagcataca
     2101 cccattgagg atggtaataa atcgccaaaa aagaagagaa aggtcgacgg cggtggtgct
     2161 ttgtctcctc agcactctgc tgtcactcaa ggaagtatca tcaagaacaa ggagggcatg
     2221 gatgctaagt cactaactgc ctggtcccgg acactggtga ccttcaagga tgtatttgtg
     2281 gacttcacca gggaggagtg gaagctgctg gacactgctc agcagatcgt gtacagaaat
     2341 gtgatgctgg agaactataa gaacctggtt tccttgggtt atcagcttac taagccagat
     2401 gtgatcctcc ggttggagaa gggagaagag ccctggctgg tggagagaga aattcaccaa
     2461 gagacccatc ctgattcaga gactgcattt gaaatcaaat catcagttta aagatccaga
     2521 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     2581 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     2641 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     2701 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atcctgcaag
     2761 cctcgtcgtc ctggccggac cacgctatct gtgcaaggtc cccggccccg gacgcgcgag
     2821 cttggcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
     2881 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
     2941 tcaatgtacc tataaccaga ccgttcagtt gcggccgcta actataacgg tcctaaggta
     3001 gcgagcgatc gcggtaccca gcttttgttc cctttagtga gggttaattg cgcgcttggc
     3061 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     3121 catacgagcc gggagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     3181 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     3241 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     3301 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     3361 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     3421 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     3481 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     3541 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     3601 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     3661 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     3721 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     3781 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     3841 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     3901 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     3961 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     4021 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     4081 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     4141 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     4201 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     4261 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     4321 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     4381 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     4441 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     4501 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     4561 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     4621 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     4681 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     4741 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     4801 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     4861 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     4921 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     4981 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     5041 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     5101 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     5161 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     5221 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     5281 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     5341 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     5401 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     5461 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     5521 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     5581 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     5641 acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc
     5701 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     5761 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     5821 aaaacgacgg ccagtgagcg cgcgtaatac gactcactat agggcgaatt ggagctcgcg
     5881 atcgcattac cctgttatcc ctagtccaag cgatcgcggc cgggtcgaca taacttcgta
     5941 tagcatacat tatacgaagt tatccggcca aggcccgcgg ggccactagt tctagagcga
     6001 tccccgcggt gcgggtgcca gggcgtgccc ttgggctccc cgggcgcgta ctccacggat
     6061 cgccaccgcg gtggagctgt cgacataact tcgtatagca tacattatac gaagttatag
     6121 ctccaattcg ccctatagtg agtcgtatta cgcgcggaca ttaccctgtt atccctaggc
     6181 gcgccgtcga cgccccagct ggttctttcc gcctcagaag ccatagagcc caccgcatcc
     6241 ccagcatgcc tgctattgtc ttcccaatcc tcccccttgc tgtcctgccc caccccaccc
     6301 cccagaatag aatgacacct actcagacaa tgcgatgcaa tttcctcatt ttattaggaa
     6361 aggacagtgg gagtggcacc ttccagggtc aaggaaggca cgggggaggg gcaaacaaca
     6421 gatggctggc aactagaagg cacagtcgag gctgatcagc gtcgagcggc cggccttaaa
     6481 agtgcctctt catgtgtaag gcaaggtggt ccgacctgga aaaggccctg tcgcacttct
     6541 ggcactgaaa gggccggtgc cctgtgtgtt tgcggtagtg cctggtcagt tcatccgagc
     6601 gggcgaattt ccacccacag ccgtcccagt cacagtggta aggtttctcg cctgtgtgag
     6661 ttcgcaggtg tgccttgaga tgagaactct tggtataggt tttgccacag cctgcatagt
     6721 cacaagtgtg ggtggctgtt cttttccggg gccacgacct tcttcccctc tttggcttgg
     6781 gctcctctgg caggcaggaa cccggtggca tgagctcttg ataatggaga gaggggactt
     6841 gtgactgcat ctggtctggc aggaaaggag ggtagttggg ccccggatgg ggatggaatc
     6901 ctgggggaag aggcaggcca gggtgacagt ccctgctgtt cagcagttcc tcgggactca
     6961 gtgtaggggt agtcctggtg gggagctgcc gccccagggg gaagtcgtgt gtgttgggcc
     7021 ggtggccgtt gctgagctgg ggtccagcgc tcaaatgggc ctctagggac cggctgaccg
     7081 tgcaggacgg gaccgcctct tgcttaatct tggggcacat gcgcggcggg ccaccgctgt
     7141 agggcgccac taccacgggg tggctgccgt ctgggcttcc tttgctaaca ctgatgaccg
     7201 aagggctgct gtactcgctg ccaggggtgg tcagagacgc cttcagcaca aacttgccca
     7261 tcagcccgcc acctggcggc tgaggctgct gtggcggaat gtatactggg tccaactccg
     7321 gccgcaggag ctcagccacg aagccgcccg aggggctcac gtcattgatg tccgccaggt
     7381 tgaagggggc cgtgggaggt ggcgcagatt ctcggctgta gaggagccct ccacctgtgt
     7441 tgctggcagc cacgcccggg tcacccccgg cccggatcgg atagctgaag ctgcaggtgg
     7501 agggcgcgct ggcagggccg ctgctcgctg gggaagacga ggatgaagct gacgccgagg
     7561 tggtcacggt ggcggccacc gattcctggt gggttagcga gttggaaagg ataaagtcta
     7621 ggtccaggag gtcgttgaac tcctcggtct ccctccgggc taggagggcc gggttgttac
     7681 tgctgcaagc tgcaccagct ccgccactct ccaggtctgt ggccaccgtc gccgccaggt
     7741 cgtaggggcg gccgggaagt gggggaagtc gcttcatgtg agagagttcc tcacgccaac
     7801 ggttagtcgg ggcacctgct ggacgcagtg tcttctccct tcccgccggg ccggacgcga
     7861 acgtggagaa ggacgggagc agagcgtcgc tgacagccat agggccggga ttctcctcca
     7921 cgtcaccgca tgttagaaga cttcctctgc cctccatgtg cgacaggggc agtgtgccgt
     7981 taatggccgt gccgggcacc gggccgctct ggtagtgctg ggccatgtgc agtctactgg
     8041 gcgcagcggg ctccggcacc tcggcgccgg ggaggtacat gctgatcatg tcccggaggt
     8101 ccccggcctg gcagggcgcc ctggagtggg aggaagaggt aaccacgggg gggctggagc
     8161 tggcctcgga cttgaccaca gagcccatgg agcccagcgc cataccgggg gtgccctgct
     8221 gcgagtagga catgctgtag gtgggcgagc cgttcatgta ggtctgcgag ctggtcatgg
     8281 agttgtactg cagggcgctg acgtcgtagc ggtgcatcgg ttgcatctgt gccgcgccgt
     8341 gagcgttgag gcccgggtgc tgcgggtagc ccagctgctc ctgcatcatg ctgtagctgc
     8401 cgttgctcca gccgttcatg tgcgcgtagc tgtccatgcg ctggttcacg cccgcaccca
     8461 ggccggcgcc caccccaacc ccgctcgcca tgctgttccc gccgggggcc agcaagcctc
     8521 cgggaagcgt gtacttatcc ttcttcatga gcgtcttggt tttccgccgc ggccggtatt
     8581 tataatccgg gtgctccttc atgtgcagag cgcgcagccg cttggcctcg tcgatgaacg
     8641 gccgcttctc ggtctcggac aaaagtttcc actccgcgcc caggcgcttg ctgatctccg
     8701 agttgtgcat cttggggttc tcctgggcca tcttacgccg ctgcccccgg gaccatacca
     8761 tgaaggcgtt catgggcctc ttgacgcggt ccgggctgtt cttctggttg ccgccggtcg
     8821 ccgccgccgt ggcgttgcct cctccgccgc cgccccccga agcttgctgc gggcccggcg
     8881 gcttcagctc cgtctccatc atgttataca tcatggtggc ggcggccggc cgccagtgtg
     8941 atggatggat atccgcagga tccgcttggg ctgcagttgg acctgggagt ggacacctgt
     9001 ggagagaaag gcaaagtgga tgtcattgtc actcaagtgt atggccagat ctcaagcctg
     9061 ccacacctca agcttgacaa caaaaagatt gtcttttctg accagatgga cgcggccacc
     9121 ctcaaaggca tcaccgcggg ccaggtgaat atcaaatcct cctcgttttt ggaaactgac
     9181 aatcttagcg cagaagtcat gcccgctttt gagagggagt actcacccca acagctggcc
     9241 ctcgcagaca gcgaattgtc gacgaattcg gggccgcgga ggctggatcg gtcccggtgt
     9301 cttctatgga ggtcaaaaca gcgtggatgg cgtctccagg cgatctgacg gttcactaaa
     9361 cgagctctgc ttatataggt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     9421 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
     9481 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     9541 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     9601 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     9661 agaaaagtga aagtcgagct cggtacccgg gtcgaggtag gcgtgtacgg tgggaggcct
     9721 atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt
     9781 ttgacctcca tagaagacac cgggaccgat ccagcctccg cggccccgaa ttcttaatta
     9841 aaattcgctg tctgcgaggg ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     9901 gacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     9961 cgcggtgatg cctttgaggg tggccgcgtc catctggtca gaaaagacaa tctttttgtt
    10021 gtcaagcttg aggtgtggca ggcttgagat ctggccatac acttgagtga caatgacatc
    10081 cactttgcct ttctctccac aggtgtccac tcccaggtcc aactgcagcc caagcggatc
    10141 ctgcggatat ccatccatca cactggcggc cggccgccgc caccatggct ggacacctgg
    10201 cttcagactt cgccttctca cccccaccag gtgggggtga tgggtcagca gggctggagc
    10261 cgggctgggt ggatcctcga acctggctaa gcttccaagg gcctccaggt gggcctggaa
    10321 tcggaccagg ctcagaggta ttggggatct ccccatgtcc gcccgcatac gagttctgcg
    10381 gagggatggc atactgtgga cctcaggttg gactgggcct agtcccccaa gttggcgtgg
    10441 agactttgca gcctgagggc caggcaggag cacgagtgga aagcaactca gagggaacct
    10501 cctctgagcc ctgtgccgac cgccccaatg ccgtgaagtt ggagaaggtg gaaccaactc
    10561 ccgaggagtc ccaggacatg aaagccctgc agaaggagct agaacagttt gccaagctgc
    10621 tgaagcagaa gaggatcacc ttggggtaca cccaggccga cgtggggctc accctgggcg
    10681 ttctctttgg aaaggtgttc agccagacca ccatctgtcg cttcgaggcc ttgcagctca
    10741 gccttaagaa catgtgtaag ctgcggcccc tgctggagaa gtgggtggag gaagccgaca
    10801 acaatgagaa ccttcaggag atatgcaaat cggagaccct ggtgcaggcc cggaagagaa
    10861 agcgaactag cattgagaac cgtgtgaggt ggagtctgga gaccatgttt ctgaagtgcc
    10921 cgaagccctc cctacagcag atcactcaca tcgccaatca gcttgggcta gagaaggatg
    10981 tggttcgagt atggttctgt aaccggcgcc agaagggcaa aagatcaagt attgagtatt
    11041 cccaacgaga agagtatgag gctacaggga cacctttccc agggggggct gtatcctttc
    11101 ctctgccccc aggtccccac tttggcaccc caggctatgg aagcccccac ttcaccacac
    11161 tctactcagt cccttttcct gagggcgagg cctttccctc tgttcccgtc actgctctgg
    11221 gctctcccat gcattcaaac gagggcagag gaagtcttct aacatgcggt gacgtggagg
    11281 agaatcccgg ccctatgccc ctcaacgtga acttcaccaa caggaactat gacctcgact
    11341 acgactccgt acagccctat ttcatctgcg acgaggaaga gaatttctat caccagcaac
    11401 agcagagcga gctgcagccg cccgcgccca gtgaggatat ctggaagaaa ttcgagctgc
    11461 ttcccacccc gcccctgtcc ccgagccgcc gctccgggct ctgctctcca tcctatgttg
    11521 cggtcgctac gtccttctcc ccaagggaag acgatgacgg cggcggtggc aacttctcca
    11581 ccgccgatca gctggagatg atgaccgagt tacttggagg agacatggtg aaccagagct
    11641 tcatctgcga tcctgacgac gagaccttca tcaagaacat catcatccag gactgtatgt
    11701 ggagcggttt ctcagccgct gccaagctgg tctcggagaa gctggcctcc taccaggctg
    11761 cgcgcaaaga cagcaccagc ctgagccccg cccgcgggca cagcgtctgc tccacctcca
    11821 gcctgtacct gcaggacctc accgccgccg cgtccgagtg cattgacccc tcagtggtct
    11881 ttccctaccc gctcaacgac agcagctcgc ccaaatcctg tacctcgtcc gattccacgg
    11941 ccttctctcc ttcctcggac tcgctgctgt cctccgagtc ctccccacgg gccagccctg
    12001 agcccctagt gctgcatgag gagacaccgc ccaccaccag cagcgactct gaagaagagc
    12061 aagaagatga ggaagaaatt gatgtggtgt ctgtggagaa gaggcaaacc cctgccaaga
    12121 ggtcggagtc gggctcatct ccatcccgag gccacagcaa acctccgcac agcccactgg
    12181 tcctcaagag gtgccacgtc tccactcacc agcacaacta cgccgcaccc ccctccacaa
    12241 ggaaggacta tccagctgcc aagagggcca agttggacag tggcagggtc ctgaagcaga
    12301 tcagcaacaa ccgcaagtgc tccagcccca ggtcctcaga cacggaggaa aacgacaaga
    12361 ggcggacaca caacgtcttg gaacgtcaga ggaggaacga gctgaagcgc agcttttttg
    12421 ccctgcgtga ccagatccct gaattggaaa acaacgaaaa ggcccccaag gtagtgatcc
    12481 tcaaaaaagc caccgcctac atcctgtcca ttcaagcaga cgagcacaag ctcacctctg
    12541 aaaaggactt attgaggaaa cgacgagaac agttgaaaca caaactcgaa cagcttcgaa
    12601 actctggtgc ataataaacg tcgagcagca cgaggtggct gtggccagat actgcgacct
    12661 ccctagcaaa ctggggcaca aacttaattg aggccggccg ctcgacgctg atcagcctcg
    12721 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
    12781 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
    12841 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
    12901 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
    12961 agaaccagct ggggcttaat taaggcgcgc cggattcgac attgattatt gactagttat
    13021 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
    13081 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
    13141 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
    13201 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
    13261 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
    13321 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
    13381 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc accccgtccc
    13441 aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg
    13501 cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc
    13561 gccgccgtcc ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc
    13621 gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct
    13681 ctgctaacca tgttcatgcc ttcttctttt tcctacag
//
