LOCUS       sequence-172502-        4612 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express zebrafish recoverin1a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Zang J, Keim J, Kastenhuber E, Gesemann M, Neuhauss SC
  TITLE     Recoverin depletion accelerates cone photoresponse recovery.
  JOURNAL   Open Biol. 2015 Aug;5(8). pii: 150086. doi: 10.1098/rsob.150086.
  PUBMED    26246494
REFERENCE   2  (bases 1 to 4612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Open 
            Biol."; date: "2015-08"; volume: "5(8). pii: 150086. doi"; pages: " 
            10.1098/rsob.150086"
COMMENT     Sequence Label: recoverin1a
FEATURES             Location/Qualifiers
     source          1..4612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     172..191
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(427..1221)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(538..557)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1148..1167
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     rep_origin      complement(1565..1993)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1675..1696)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1887..1906
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2119..2141
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2133..2150
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2134..2150
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2157..2176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2157..2175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2964..2982)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2965..2982)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3000..3016)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3000..3016)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3013..3035)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3024..3040
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3048..3054,3055..3072,3073..3078))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3093..3114
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3231..3248)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3402..3990)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3482..3501)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4161..4612,1..340,341..409))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
       61 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
      121 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
      181 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
      241 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
      301 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
      361 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
      421 ttcaattcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc
      481 ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat
      541 atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc
      601 gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg
      661 ggtcacgacg agatcctcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc
      721 tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat
      781 ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg
      841 atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc
      901 aaggtgggat gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc
      961 cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga
     1021 tagccgcgct gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa
     1081 aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt
     1141 ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg
     1201 caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat cagatcttga
     1261 tcccctgcgc catcagatcc ttggcggcaa gaaagccatc cagtttactt tgcagggctt
     1321 cccaacctta ccagagggcg ccccagctgg caattccggt tcgcttgctg tccataaaac
     1381 cgcccagtct agctatcgcc atgtaagccc actgcaagct acctgctttc tctttgcgct
     1441 tgcgttttcc cttgtccaga tagcccagta gctgacattc atccggggtc agcaccgttt
     1501 ctgcggactg gctttctacg tgttccgctt cctttagcag cccttgcgcc ctgaattttg
     1561 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     1621 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     1681 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     1741 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     1801 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     1861 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     1921 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     1981 ctacagggcg cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     2041 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     2101 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat
     2161 acgactcact atagggcgaa ttgggccctc tagatgcatg ctcgagcggc cgccagtgtg
     2221 atggatatct gcagaattcg cccttaaaaa gctagctatg gggaatacta agagtggggc
     2281 tttgtccaaa gagcttctgg aggaccttaa gctaaacacc aaatacacag aagaagagct
     2341 ctgcgcatgg tacaccagct ttctcaaaga gtgtccctct ggacgtatta ccaaagaaca
     2401 gtttgagggc atctatgcaa gtttcttccc ggatgctgac cctacagcat atgctaggca
     2461 cgtcttccgc agctttgaca ccaacgctga tggcactttg ggctttaagg agtacattgt
     2521 ggcactgcac ctcacctctt caggcaagac cctgcgcaaa cttgaatggg cctttgcttt
     2581 gtacgatgtt gatggaaatg ggaccatcag taaaaatgag gtgcaggaga ttgtacggtc
     2641 gatattcaac atggttcctg ttgaagacca gaaaaacctt ccagatgatg agaacacgcc
     2701 agaaaagcga gcagacaaaa tctgggcttt tttcggaaag caagataatg ataagattgg
     2761 agagggggaa ttcattcagg gtgtcacgga gaacaaagac atattgaggt tgatacaata
     2821 tgatgagcca aagaagattc aagaaaaact caaagaaaag aagcattaag cggccgcaaa
     2881 aaaaaagggc gaattccagc acactggcgg ccgttactag tggatccgag ctcggtacca
     2941 agcttgatgc atagcttgag tattctatag tgtcacctaa atagcttggc gtaatcatgg
     3001 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     3061 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     3121 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     3181 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     3241 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     3301 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     3361 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     3421 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     3481 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     3541 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     3601 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     3661 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     3721 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     3781 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     3841 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     3901 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     3961 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     4021 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     4081 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     4141 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     4201 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     4261 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     4321 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     4381 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     4441 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     4501 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     4561 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt ca
//
