LOCUS       sequence-172526-        1135 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector expressing shRNA against murine DNMT1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Leoni C, Montagner S, Rinaldi A, Bertoni F, Polletti S, Balestrieri 
            C, Monticelli S
  TITLE     Dnmt3a restrains mast cell inflammatory responses.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Feb 6. pii: 201616420. doi: 
            10.1073/pnas.1616420114.
  PUBMED    28167789
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2017 Feb 6. pii: 201616420. doi: 
            10.1073/pnas.1616420114."
COMMENT     Sequence Label: pAPM sh1 DNMT1
FEATURES             Location/Qualifiers
     source          1..1135
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             173..772
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(173..192)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     669..689
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     ncRNA           822..943
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 ctcccccaag gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc agcctgcttc
       61 tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa aagagctcac aacccctcac
      121 tcggcgcgcc agtcctccga cagactgagt cgcccggggg tctagagcca ccatgaccga
      181 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct
      241 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat
      301 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
      361 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
      421 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
      481 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
      541 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
      601 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
      661 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
      721 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gagcggccgc
      781 aagccttgtt aagtgctcgt tcggcagcac atatactatg tttgaatgag gcttcagtac
      841 tttacagaat cgttgcctgc acatcttgga aacacttgct gggattactt cttcaggtta
      901 acccaacaga aggctcgaga aggtatattg ctgttgacag tgagcgcaca aacgctctca
      961 tcgacaagtg agtgtgtgag ggagaaaact tgtcgatgag agcgtttgta tgcctactgc
     1021 ctcggaattc aaggggctac tttaggagca attatcttgt ttactaaaac tgaatacctg
     1081 ctatctcttt gatacatttt tacaaagctg aattaaaatg gtataaatta aatca
//
