LOCUS       sequence-172528-        5446 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BAR domain of human Amphiphysin1 (amino acids 1-256), C-terminally 
            tagged with mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   Hayer A, Shao L, Chung M, Joubert LM, Yang HW, Tsai FC, Bisaria A, 
            Betzig E, Meyer T
  TITLE     Engulfed cadherin fingers are polarized junctional structures 
            between collectively migrating endothelial cells.
  JOURNAL   Nat Cell Biol. 2016 Dec;18(12):1311-1323. doi: 10.1038/ncb3438. Epub
            2016 Nov 14.
  PUBMED    27842057
REFERENCE   2  (bases 1 to 5446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3438"; 
            journalName: "Nat Cell Biol"; date: "2016-12"; volume: "18"; issue: 
            "12"; pages: "1311-1323"
COMMENT     Sequence Label: pCAG-AMPH1-BAR-mCherry
FEATURES             Location/Qualifiers
     source          1..5446
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     256..278
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     340..359
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1164..1173
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1170..1880
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1315..1333)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1583..1602
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(1978..1997)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2043..2098
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2097..2116)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2459..2475)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2459..2475)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2472..2494)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2483..2499
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2507..2513,2514..2531,2532..2537))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2552..2573
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2632..2827
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2678..2813
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2833..2967
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2870..2889)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2924..2943
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3035..3052)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3206..3794)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3286..3305)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3965..4756,4757..4825))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4588..4607
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4826..4930)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        4961..5340
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct
       61 gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc
      121 ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc
      181 gtccccttct ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg
      241 gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta
      301 accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc
      361 tgtctcatca ttttggcaaa gaattcatgg ccgacatcaa gacgggcatc ttcgccaaga
      421 acgtccagaa gcgactcaac cgcgcgcagg aaaaggtcct ccaaaagctg gggaaagctg
      481 atgagacaaa agacgaacag ttcgaagaat atgtccagaa cttcaaacgg caagaagcag
      541 agggtaccag acttcagcga gaactccgag gatatttagc agcaatcaaa ggcatgcagg
      601 aggcctccat gaagctcaca gagtcgctgc atgaagtcta tgagcctgac tggtatgggc
      661 gggaagatgt gaaaatggtt ggtgagaaat gtgatgtgct gtgggaagac ttccatcaaa
      721 aactcgtgga tgggtccttg ctaacactgg atacctacct ggggcaattt cctgacataa
      781 agaatcgcat cgccaagcgc agcaggaagc tagtggacta tgacagtgcc cgccaccatc
      841 tggaagctct gcagagctcc aagaggaagg atgagagtcg aatctctaag gcagaagaag
      901 aatttcagaa agcacagaaa gtgtttgaag agtttaacgt tgacttacaa gaagagttac
      961 catcattatg gtcaagacga gttggatttt atgttaatac tttcaaaaac gtctccagcc
     1021 ttgaagccaa gtttcataag gaaattgcgg tgctttgcca caaactgtat gaagtgatga
     1081 caaaactggg tgaccagcac gccgacaagg ccttcaccat ccaaggagcg cccagtgatt
     1141 cgggtcctct ccgcggaccg gtcgccacca tggtgagcaa gggcgaggag gataacatgg
     1201 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
     1261 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     1321 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
     1381 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
     1441 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
     1501 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
     1561 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     1621 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga
     1681 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
     1741 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg
     1801 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca
     1861 tggacgagct gtacaagtaa ctcgaggata tcgcggccgc actcctcagg tgcaggctgc
     1921 ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct
     1981 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg
     2041 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact
     2101 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag
     2161 tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca
     2221 tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg
     2281 aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt
     2341 ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc
     2401 tgtccctctt ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt
     2461 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     2521 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     2581 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa
     2641 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     2701 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     2761 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     2821 ttgcaaaaag ctaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     2881 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     2941 atcaatgtat cttatcatgt ctggatccgc tgcattaatg aatcggccaa cgcgcgggga
     3001 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     3061 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     3121 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     3181 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     3241 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     3301 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     3361 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     3421 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3481 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3541 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3601 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     3661 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     3721 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     3781 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     3841 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     3901 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     3961 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     4021 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     4081 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     4141 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     4201 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     4261 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     4321 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     4381 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     4441 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     4501 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     4561 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     4621 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     4681 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     4741 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     4801 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     4861 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     4921 gggttccgcg cacatttccc cgaaaagtgc cacctgggtc gacattgatt attgactagt
     4981 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     5041 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     5101 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     5161 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     5221 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     5281 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     5341 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca
     5401 attttgtatt tatttatttt ttaattattt tgtgcagcga tggggg
//
