LOCUS       sequence-172563-        1119 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector expressing the fusion protein HA-NFAT1 
            (4-460)-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Leoni C, Montagner S, Rinaldi A, Bertoni F, Polletti S, Balestrieri 
            C, Monticelli S
  TITLE     Dnmt3a restrains mast cell inflammatory responses.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Feb 6. pii: 201616420. doi: 
            10.1073/pnas.1616420114.
  PUBMED    28167789
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2017 Feb 6. pii: 201616420. doi: 
            10.1073/pnas.1616420114."
COMMENT     Sequence Label: pSCALPs-HA-NFAT1 (4-460)-GFP
FEATURES             Location/Qualifiers
     source          1..1119
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      310..319
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(316..318,319..321,322..1035)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(316..318,319..321,322..1032)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(361..382)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(622..641)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     969..990
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aggaggaatt ccgctccaga gtccatcctg ctggtaccac ctacttggcc caagcagttg
       61 gtgccggcca ttcccatctg cagcatccct gtgactgcat ccctcccacc actcgagtgg
      121 ccactctcca atcagtcggg ctcctatgag ctacggattg aggtccaacc caagccccat
      181 caccgggccc actatgagac ggagggcagc cgtggcgctg tcaaagcccc aacaggagga
      241 caccctgtgg tgcagctcca cggctacatg gagaacaagc ctctggggct tcagatcgat
      301 ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
      361 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
      421 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
      481 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
      541 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
      601 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
      661 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
      721 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
      781 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
      841 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
      901 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
      961 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     1021 gagctgtaca agtaaagcgg ccgcgactct agttaacaat cagccataac tggccagaat
     1081 tctcgacctt gactggcggc gcgaccttga ggcctgcgt
//
