LOCUS       sequence-172566-        4640 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of cyclin D1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Matsushime H, Roussel MF, Ashmun RA, Sherr CJ
  TITLE     Colony-stimulating factor 1 regulates novel cyclins during the G1 
            phase of the cell cycle.
  JOURNAL   Cell. 1991 May 17;65(4):701-13.
  PUBMED    1827757
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "1991-05-17"; volume: "65(4)"; pages: "701-13"
COMMENT     Sequence Label: pFLEX+mCyclin D1
FEATURES             Location/Qualifiers
     source          1..4640
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..24
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    1238..1372
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1262..1281)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1316..1335
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1515..1537)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1623..1763
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1678..1697
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1778..1795)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1949..2537)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2029..2048)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2708..3499,3500..3568))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3331..3350
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3569..3673)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3741..3759
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3829..3849)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(3834..4163,4164..4178,4179..4447)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      4014..4149
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             4179..4447
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          4491..4587
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
ORIGIN
        1 gactacaagg acgacgatga caagtctcga gcaattgaat tcggccgcgc catggaacac
       61 cagctcctgt gctgcgaagt ggagaccatc cgccgcgcgt accctgacac caatctcctc
      121 aacgaccggg tgctgcgagc catgctcaag acggaggaga cctgtgcgcc ctccgtatct
      181 tacttcaagt gcgtgcagaa ggagattgtg ccatccatgc ggaaaatcgt ggccacctgg
      241 atgctggagg tctgtgagga gcagaagtgc gaagaggagg tcttcccgct ggccatgaac
      301 tacctggacc gcttcctgtc cctggagccc ctgaagaaga gccgcctgca gctgctgggg
      361 gccacctgca tgttcgtggc ctctaagatg aaggagacca ttcccttgac tgccgagaag
      421 ttgtgcatct acactgacaa ctctatccgg cccgaggagc tgctgcaaat ggaactgctt
      481 ctggtgaaca agctcaagtg gaacctggcc gccatgactc cccacgattt catcgaacac
      541 ttcctctcca aaatgccaga ggcggatgag aacaagcaga ccatccgcaa gcatgcacag
      601 acctttgtgg ccctctgtgc cacagatgtg aagttcattt ccaacccacc ctccatggta
      661 gctgctggga gcgtggtggc tgcgatgcaa ggcctgaacc tgggcagccc caacaacttc
      721 ctctcctgct accgcacaac gcactttctt tccagagtca tcaagtgtga cccggactgc
      781 ctccgtgcct gccaggaaca gattgaagcc cttctggagt caagcctgcg ccaggcccag
      841 cagaacgtcg accccaaggc cactgaggag gagggggaag tggaggaaga ggctggtctg
      901 gcctgcacgc ccaccgacgt gcgagatgtg gacatctgag ggccaccggg caggcgggag
      961 ccaccaagta gtggcacccg caaagaggaa ggagccagcc cgggtgctcc tgacgacgtc
     1021 ccccttgggg acatgttgtt accagaagag gaagttttgt tctctttgtt ggttgttttt
     1081 ccttaatctt tctcctttct atctgattta agcaaaagag aaaaaaatat ctgaaagctg
     1141 tcttaaagag agagagagag agagatagaa tctgcatcac cctgagagta gggagccagg
     1201 gggtgctaca aaaatagaat tcctgattga ttgaccggat ccagacatga taagatacat
     1261 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     1321 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     1381 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
     1441 gtaaaacctc tacaaatgtg gtatggctga ttatgatccg gctgcctcgc gcgtttcggt
     1501 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     1561 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     1621 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
     1681 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     1741 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     1801 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     1861 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     1921 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     1981 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     2041 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2101 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     2161 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     2221 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2281 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2341 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     2401 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2461 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2521 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     2581 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     2641 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     2701 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     2761 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     2821 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     2881 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     2941 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     3001 tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg
     3061 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     3121 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     3181 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     3241 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     3301 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa
     3361 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     3421 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     3481 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     3541 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     3601 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     3661 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     3721 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaattgg
     3781 tcgaccaatt ctcatgtttg acagcttatc atcgataagc ttggctgtgg aatgtgtgtc
     3841 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     3901 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     3961 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     4021 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     4081 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     4141 ttggaggcct aggcttttgc aaaaagctcc tcgatcgagg ggctcgcatc tctccttcac
     4201 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
     4261 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
     4321 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
     4381 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
     4441 acagatccaa gctcctcgat cgaggaactg aaaaaccaga aagttaactg gtaagtttag
     4501 tctttttgtc ttttatttca ggtcccggat ccggtggtgg tgcaaatcaa agaactgctc
     4561 ctcagtggat gttgccttta cttctaggcc tgtacggaag tgttacttct gctctaaaag
     4621 ctgctctagc cctcgccatg
//
