LOCUS       sequence-172567-       13536 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Disturb the expression of miR408 in soybean.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 13536)
  AUTHORS   Yan J, Gu Y, Jia X, Kang W, Pan S, Tang X, Chen X, Tang G
  TITLE     Effective small RNA destruction by the expression of a short tandem 
            target mimic in Arabidopsis.
  JOURNAL   Plant Cell. 2012 Feb;24(2):415-27. doi: 10.1105/tpc.111.094144. Epub
            2012 Feb 17.
  PUBMED    22345490
REFERENCE   2  (bases 1 to 13536)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.111.094144"; journalName: "Plant Cell"; date: 
            "2012-02"; volume: "24"; issue: "2"; pages: "415-27"
COMMENT     Sequence Label: Gma-pCAMBIA-1301-STTM408
FEATURES             Location/Qualifiers
     source          1..13536
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        208..254
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     polyA_signal    416..592
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1154..1172)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1155..1172)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1811..1830
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(1933..1938,1939..1955,1956..1961))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(2220..2237)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2220..2236)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2229..2251)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        2613..2958
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(2975..2989,3180..4997)
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUS
                     /note="This version of the gusA gene has a 5' extension 
                     with a catalase intron to ensure expression in plants but 
                     not bacteria."
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWES
                     ALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGK
                     VWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
                     KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSV
                     ELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRS
                     VAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYP
                     YAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQA
                     IKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKK
                     GIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     intron          2990..3179
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             5004..5021
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      5056..5308
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5330..5354
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6655..7284
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7713..8786
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8852..9046
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9282..9304)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9390..9530
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9445..9464
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9545..9562)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9716..10304)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10391..11185)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11610..11634
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    11712..11886
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(11927..12952)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(13025..13370)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
ORIGIN
        1 ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggctatca ttcaagatgc
       61 ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga
      121 agacgttcca accacgtctt caaagcaagt ggattgatgt gacatctcca ctgacataag
      181 ggatgacgca caaccccact atccttcgca agacccttcc tctatataag gaagttcatt
      241 tcatttggag aggacagccc aagcttcatg ctctgcccta tgttcccctg gttgttgttg
      301 ttatggtcta atttaaatat ggtctaaaga agaagaatcg tgccctgcct actgttccca
      361 ggaattcggt acgctgaaat caccagtctc tctctacaaa tctatctctc tctattttct
      421 ccataaataa tgtgtgagta gtttcccgat aagggaaatt agggttctta tagggtttcg
      481 ctcatgtgtt gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta
      541 tcaataaaat ttctaattcc taaaaccaaa atccagtact aaaatccaga tctcctaaag
      601 tccctataga tctttgtcgt gaatataaac cagacacgag acgactagac ctggagccca
      661 gacgccgttc gaagctagaa gtaccgctta ggcaggaggc cgttagggaa aagatgctaa
      721 ggcagggttg gttacgttga ctcccccgta ggtttggttt aaatatgatg aagtggacgg
      781 aaggaaggag gaagacaagg aaggataagg ttgcaggccc tgtgcaaggt aagaagatgg
      841 aaatttgata gaggtacgct actatactta tactatacgc taagggaatg attgtattta
      901 taccctatac cccctaataa ccccttatca atttaagaaa taatccgcat aagcccccgc
      961 ttaaaaattg gtatcagagc catgaatagg tctatgacca aaactcaaga ggataaaacc
     1021 tcaccaaaat acgaaagagt ccttaactct aaagataaaa gatctttcaa gatcaaaact
     1081 agttccctca caccggtgac ggggatcgca tgcgacctct agaccatcac attggagctt
     1141 cagctgctcg agttctatag tgtcacctaa atcgtatgtg tatgatacat aaggttatgt
     1201 attaattgta gccgcgttct tacgtcgacg taccaattac gccccgccct gtcactcatc
     1261 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg
     1321 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
     1381 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
     1441 actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata
     1501 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
     1561 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
     1621 gtaacaaggg tgaacactat cccatattac cagctcaccg tctttcattg ccatacggaa
     1681 ctccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
     1741 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
     1801 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat
     1861 atcaacggtg gtatatccag tgatttcttt ctccatttta gcttccttag ctcctgaaaa
     1921 tctcgataac tcattaaagc ttatcgatga taagctgtca aacatgagaa tttagcccgc
     1981 ctaatgagcg ggctttttgt tgtgatccgc gttgctggcg tttttccata ggctccgccc
     2041 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2101 ataaagatac caggcgtttc ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     2161 tgccgcttac cggatacctg tccgcctttc ttaattaact gcaggcatgc aagcttggca
     2221 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     2281 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     2341 ccttcccaac agttgcgcag cctgaatggc gaatgctaga gcagcttgag cttggatcag
     2401 attgtcgttt cccgccttca gtttagcttc atggagtcaa agattcaaat agaggaccta
     2461 acagaactcg ccgtaaagac tggcgaacag ttcatacaga gtctcttacg actcaatgac
     2521 aagaagaaaa tcttcgtcaa catggtggag cacgacacac ttgtctactc caaaaatatc
     2581 aaagatacag tctcagaaga ccaaagggca attgagactt ttcaacaaag ggtaatatcc
     2641 ggaaacctcc tcggattcca ttgcccagct atctgtcact ttattgtgaa gatagtggaa
     2701 aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat
     2761 gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa
     2821 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta
     2881 agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca
     2941 tttcatttgg agagaacacg ggggactctt gaccatggta gatctgaggg taaatttcta
     3001 gtttttctcc ttcattttct tggttaggac ccttttctct ttttattttt ttgagctttg
     3061 atctttcttt aaactgatct attttttaat tgattggtta tggtgtaaat attacatagc
     3121 tttaactgat aatctgatta ctttatttcg tgtgtctatg atgatgatga tagttacaga
     3181 accgacgact cgtccgtcct gtagaaaccc caacccgtga aatcaaaaaa ctcgacggcc
     3241 tgtgggcatt cagtctggat cgcgaaaact gtggaattga tcagcgttgg tgggaaagcg
     3301 cgttacaaga aagccgggca attgctgtgc caggcagttt taacgatcag ttcgccgatg
     3361 cagatattcg taattatgcg ggcaacgtct ggtatcagcg cgaagtcttt ataccgaaag
     3421 gttgggcagg ccagcgtatc gtgctgcgtt tcgatgcggt cactcattac ggcaaagtgt
     3481 gggtcaataa tcaggaagtg atggagcatc agggcggcta tacgccattt gaagccgatg
     3541 tcacgccgta tgttattgcc gggaaaagtg tacgtatcac cgtttgtgtg aacaacgaac
     3601 tgaactggca gactatcccg ccgggaatgg tgattaccga cgaaaacggc aagaaaaagc
     3661 agtcttactt ccatgatttc tttaactatg ccggaatcca tcgcagcgta atgctctaca
     3721 ccacgccgaa cacctgggtg gacgatatca ccgtggtgac gcatgtcgcg caagactgta
     3781 accacgcgtc tgttgactgg caggtggtgg ccaatggtga tgtcagcgtt gaactgcgtg
     3841 atgcggatca acaggtggtt gcaactggac aaggcactag cgggactttg caagtggtga
     3901 atccgcacct ctggcaaccg ggtgaaggtt atctctatga actgtgcgtc acagccaaaa
     3961 gccagacaga gtgtgatatc tacccgcttc gcgtcggcat ccggtcagtg gcagtgaagg
     4021 gcgaacagtt cctgattaac cacaaaccgt tctactttac tggctttggt cgtcatgaag
     4081 atgcggactt gcgtggcaaa ggattcgata acgtgctgat ggtgcacgac cacgcattaa
     4141 tggactggat tggggccaac tcctaccgta cctcgcatta cccttacgct gaagagatgc
     4201 tcgactgggc agatgaacat ggcatcgtgg tgattgatga aactgctgct gtcggcttta
     4261 acctctcttt aggcattggt ttcgaagcgg gcaacaagcc gaaagaactg tacagcgaag
     4321 aggcagtcaa cggggaaact cagcaagcgc acttacaggc gattaaagag ctgatagcgc
     4381 gtgacaaaaa ccacccaagc gtggtgatgt ggagtattgc caacgaaccg gatacccgtc
     4441 cgcaaggtgc acgggaatat ttcgcgccac tggcggaagc aacgcgtaaa ctcgacccga
     4501 cgcgtccgat cacctgcgtc aatgtaatgt tctgcgacgc tcacaccgat accatcagcg
     4561 atctctttga tgtgctgtgc ctgaaccgtt attacggatg gtatgtccaa agcggcgatt
     4621 tggaaacggc agagaaggta ctggaaaaag aacttctggc ctggcaggag aaactgcatc
     4681 agccgattat catcaccgaa tacggcgtgg atacgttagc cgggctgcac tcaatgtaca
     4741 ccgacatgtg gagtgaagag tatcagtgtg catggctgga tatgtatcac cgcgtctttg
     4801 atcgcgtcag cgccgtcgtc ggtgaacagg tatggaattt cgccgatttt gcgacctcgc
     4861 aaggcatatt gcgcgttggc ggtaacaaga aagggatctt cactcgcgac cgcaaaccga
     4921 agtcggcggc ttttctgctg caaaaacgct ggactggcat gaacttcggt gaaaaaccgc
     4981 agcagggagg caaacaagct agccaccacc accaccacca cgtgtgaatt acaggtgacc
     5041 agctcgaatt tccccgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct
     5101 gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata
     5161 attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa
     5221 ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg
     5281 cgcgcggtgt catctatgtt actagatcgg gaattaaact atcagtgttt gacaggatat
     5341 attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg
     5401 cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttcccctc
     5461 gggatcaaag tactttgatc caacccctcc gctgctatag tgcagtcggc ttctgacgtt
     5521 cagtgcagcc gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg cgacaggctg
     5581 ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata aagtagaata
     5641 cttgcgacta gaaccggaga cattacgcca tgaacaagag cgccgccgct ggcctgctgg
     5701 gctatgcccg cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc gaactgcacg
     5761 cggccggctg caccaagctg ttttccgaga agatcaccgg caccaggcgc gaccgcccgg
     5821 agctggccag gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg accaggctag
     5881 accgcctggc ccgcagcacc cgcgacctac tggacattgc cgagcgcatc caggaggccg
     5941 gcgcgggcct gcgtagcctg gcagagccgt gggccgacac caccacgccg gccggccgca
     6001 tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc atcgaccgca
     6061 cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc
     6121 tcaccccggc acagatcgcg cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga
     6181 aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca
     6241 gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag gacgcattga
     6301 ccgaggccga cgccctggcg gccgccgaga atgaacgcca agaggaacaa gcatgaaacc
     6361 gcaccaggac ggccaggacg aaccgttttt cattaccgaa gagatcgagg cggagatgat
     6421 cgcggccggg tacgtgttcg agccgcccgc gcacgtctca accgtgcggc tgcatgaaat
     6481 cctggccggt ttgtctgatg ccaagctggc ggcctggccg gccagcttgg ccgctgaaga
     6541 aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc
     6601 ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata cgcaagggga acgcatgaag
     6661 gttatcgctg tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta
     6721 gcccgcgccc tgcaactcgc cggggccgat gttctgttag tcgattccga tccccagggc
     6781 agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac
     6841 cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac
     6901 ggagcgcccc aggcggcgga cttggctgtg tccgcgatca aggcagccga cttcgtgctg
     6961 attccggtgc agccaagccc ttacgacata tgggccaccg ccgacctggt ggagctggtt
     7021 aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg
     7081 atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta cgagctgccc
     7141 attcttgagt cccgtatcac gcagcgcgtg agctacccag gcactgccgc cgccggcaca
     7201 accgttcttg aatcagaacc cgagggcgac gctgcccgcg aggtccaggc gctggccgct
     7261 gaaattaaat caaaactcat ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa
     7321 acacgctaag tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc
     7381 tggcagacac gccagccatg aagcgggtca actttcagtt gccggcggag gatcacacca
     7441 agctgaagat gtacgcggta cgccaaggca agaccattac cgagctgcta tctgaataca
     7501 tcgcgcagct accagagtaa atgagcaaat gaataaatga gtagatgaat tttagcggct
     7561 aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga atgccccatg
     7621 tgtggaggaa cgggcggttg gccaggcgta agcggctggg ttgcctgccg gccctgcaat
     7681 ggcactggaa cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg
     7741 tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg
     7801 cccagcggca acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg
     7861 atcgaatccg caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc
     7921 cgcccaaggg cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc
     7981 gcgatagtcg cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag
     8041 ctggcgaggt gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg
     8101 ccggcatggc cagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg
     8161 aatccatgaa ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac
     8221 acgttgcgga cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc
     8281 tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg
     8341 ccaagaacgg ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga
     8401 tcgtaaagag cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt
     8461 accgcgagat cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt
     8521 tgatcgatcc cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg
     8581 cagaagccag atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca
     8641 agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt
     8701 tgaaggagga ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg
     8761 agggcgaagc atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag
     8821 caggggaaaa aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt gggaacccaa
     8881 agccgtacat tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc
     8941 ggtcacacat gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact
     9001 ctttaaaact tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc
     9061 gcacagccga agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg
     9121 ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag
     9181 gcaatctacc agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc
     9241 aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     9301 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     9361 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc
     9421 ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     9481 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg
     9541 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     9601 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     9661 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     9721 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     9781 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     9841 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     9901 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     9961 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
    10021 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
    10081 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
    10141 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
    10201 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
    10261 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
    10321 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc
    10381 attctaggta ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag
    10441 gcttgatccc cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc
    10501 tgatcgaccg gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa
    10561 gatcaataaa gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc
    10621 cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc
    10681 gcggatcttt aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt
    10741 tattcagtaa gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat
    10801 ccgatatgtc gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt
    10861 cagggctttg ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga
    10921 gcagattgct ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc
    10981 cttccagcca tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc
    11041 ggctgtccgt catttttaaa tataggtttt cattttctcc caccagctta tataccttag
    11101 caggagacat tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt
    11161 ccggtgatat tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa
    11221 gataccccaa gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta
    11281 aaaccttaaa taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat
    11341 agtatcgacg gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg
    11401 ttacaatcaa catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt
    11461 gccgttcttc cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc
    11521 ccgctgacgc cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc
    11581 tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg
    11641 acgcttagac aacttaataa cacattgcgg acgtttttaa tgtactgaat taacgccgaa
    11701 ttaattcggg ggatctggat tttagtactg gattttggtt ttaggaatta gaaattttat
    11761 tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat
    11821 gagcgaaacc ctataggaac cctaattccc ttatctggga actactcaca cattattatg
    11881 gagaaactcg agcttgtcga tcgacagatc ccggtcggca tctactctat ttctttgccc
    11941 tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca cagccatcgg
    12001 tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg gctccggatc
    12061 ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca
    12121 agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagt cgtggcgatc
    12181 ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata caagccaacc
    12241 acggcctcca gaagaagatg ttggcgacct cgtattggga atccccgaac atcgcctcgc
    12301 tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg gagccgaaat
    12361 ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc agctcatcga
    12421 gagcctgcgc gacggacgca ctgacggtgt cgtccatcac agtttgccag tgatacacat
    12481 ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg attccttgcg
    12541 gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgatc gcatccatag
    12601 cctccgcgac cggttgtaga acagcgggca gttcggtttc aggcaggtct tgcaacgtga
    12661 caccctgtga acggcgggag atgcaatagg tcaggctctc gctaaactcc ccaatgtcaa
    12721 gcacttccgg aatcgggagc gcggccgatg caaagtgccg ataaacataa cgatctttgt
    12781 agaaaccatc ggcgcagcta tttacccgca ggacatatcc acgccctcct acatcgaagc
    12841 tgaaagcacg agattcttcg ccctccgaga gctgcatcag gtcggagacg ctgtcgaact
    12901 tttcgatcag aaacttctcg acagacgtcg cggtgagttc aggctttttc atatctcatt
    12961 gccccccgga tctgcgaaag ctcgagagag atagatttgt agagagagac tggtgatttc
    13021 agcgtgtcct ctccaaatga aatgaacttc cttatataga ggaagggtct tgcgaaggat
    13081 agtgggattg tgcgtcatcc cttacgtcag tggagatatc acatcaatcc acttgctttg
    13141 aagacgtggt tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt
    13201 tgggaccact gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc
    13261 atttgtaggt gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc
    13321 aatggaatcc gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt
    13381 ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca
    13441 tgttcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg
    13501 ctcctcgtgg gtgggggtcc atctttggga ccactg
//
