LOCUS       sequence-172570-        4105 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  bacterial expression of mouse cyclin D2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Matsushime H, Roussel MF, Ashmun RA, Sherr CJ
  TITLE     Colony-stimulating factor 1 regulates novel cyclins during the G1 
            phase of the cell cycle.
  JOURNAL   Cell. 1991 May 17;65(4):701-13.
  PUBMED    1827757
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "1991-05-17"; volume: "65(4)"; pages: "701-13"
COMMENT     Sequence Label: pBluescript-mouse cyclin D2
FEATURES             Location/Qualifiers
     source          1..4105
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(996..1012)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(997..1013)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(1037..1056)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1038..1056)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1063..1080)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1063..1079)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1072..1094)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(1221..1676)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1358..1379)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1570..1589
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        1703..1807
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1808..1876,1877..2668)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2026..2045)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2839..3427
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3328..3347
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3581..3598
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3715..3736
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3751..3756,3757..3774,3775..3781)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3789..3805
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3794..3816
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3813..3829
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3813..3829
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        3850..3868
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     3905..3921
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3905..3921
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 atggagctgc tgtgctgcga ggtggacccg gtccgcaggg ccgtgccgga ccgcaacctg
       61 ctggaagacc gcgttctgca gaacctgttg accatcgagg agcgctacct cccgcagtgt
      121 tcctatttca agtgcgtgca gaaggacatc caaccgtaca tgcgcaggat ggtggccacc
      181 tggatgctag aggtctgtga ggaacaaaag tgtgaagaag aggtctttcc tctggccatg
      241 aattacctgg accgtttctt ggctggagtc ccgactccta agacccatct tcagctcctg
      301 ggtgcagtgt gcatgttcct agcttccaag ctgaaagaga ccatcccgct gactgcggaa
      361 aagctgtgca tttacaccga caactctgtg aagccccagg agctgctgga gtgggaactg
      421 gtagtgttgg gtaagctgaa gtggaacctg gccgcagtca cccctcacga cttcattgag
      481 cacatccttc gcaagctgcc ccagcaaaag gagaagctgt ccctgatccg caagcatgcg
      541 cagaccttca tcgctctgtg cgctaccgac ttcaagtttg ccatgtaccc gccatcgatg
      601 attgcaactg gaagcgtggg agcagccatc tgtgggcttc agcaggatga tgaagtgaac
      661 acactcacgt gtgatgccct gactgagctg ctggccaaga tcacccacac tgatgtggat
      721 tgtctcaaag cctgccagga gcaaatcgaa gctctgctgc tgaacagcct gcagcagttc
      781 cgtcaagagc agcataacgc cggatccaag tctgtggaag atccggacca agccaccacc
      841 cctacagacg tgcgggatgt tgacctgtga ggaagccatt cgggcggcaa gagagaggcg
      901 tgttcgtcat ctgctagccc cttctctctc tagttatgtc ttgttctttg tgtttttagg
      961 atgaaacttc aaaaggaatt cgatatcaag cttatcgata ccgtcgacct cgaggggggg
     1021 cccggtaccc aattcgccct atagtgagtc gtattacaat tcactggccg tcgttttaca
     1081 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     1141 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     1201 cagcctgaat ggcgaatggc aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     1261 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     1321 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     1381 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     1441 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     1501 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     1561 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     1621 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg
     1681 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     1741 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     1801 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     1861 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     1921 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     1981 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     2041 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     2101 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     2161 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     2221 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     2281 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     2341 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     2401 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     2461 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     2521 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     2581 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     2641 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     2701 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     2761 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     2821 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     2881 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     2941 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     3001 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     3061 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     3121 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     3181 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     3241 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     3301 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     3361 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     3421 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     3481 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     3541 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     3601 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     3661 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     3721 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     3781 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     3841 caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc gcggtggcgg
     3901 ccgctctaga actagtggat cccccgggct gcaggaattc cctctctctc tccctctctc
     3961 tctttgccat ttctttcctc tcccaaatct cccattcagc caaaggaagg aggtaaggga
     4021 agcactcccc gacccccccg cacctccaaa aaataataat aataaaaaaa atttacagtc
     4081 gggaccgagt ggtggccggc tggct
//
