LOCUS       sequence-172572-         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For fusion of TagRFP657 to N-term of insert. SEE DEPOSITOR COMMENTS 
            BELOW..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Morozova KS, Piatkevich KD, Gould TJ, Zhang J, Bewersdorf J, 
            Verkhusha VV
  TITLE     Far-red fluorescent protein excitable with red lasers for flow 
            cytometry and superresolution STED nanoscopy.
  JOURNAL   Biophys J. 2010 Jul 21;99(2):L13-5.
  PUBMED    20643047
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biophys 
            J."; date: "2010-07-21"; volume: "99(2)"; pages: "L13-5"
COMMENT     Sequence Label: pTagRFP657-C1
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      38..47
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             44..754
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKDNMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATSFMYGSHTFINHTQGIPDFWKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEAHTEMLYPADGGLEGRTA
                     LALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDYRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 gagctggttt agtgaaccgt cagatccgct agcggtggcc accatggtgt ctaagggcga
       61 agagctgatt aaggacaaca tgcacatgaa gctgtacatg gagggcaccg tgaacaacca
      121 ccacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc agacccagag
      181 aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctaccagctt
      241 catgtacggc agtcacacct tcatcaacca cacccagggc atccccgact tctggaagca
      301 gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct
      361 gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag
      421 aggggtgaac ttcccatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc
      481 ccacaccgag atgctgtacc ccgctgacgg cggcctggaa ggcagaaccg cgctggccct
      541 gaagctcgtg ggcgggggcc acctgatctg caacttcaag accacataca gatccaagaa
      601 acccgctaag aacctcaaga tgcccggcgt ctactatgtg gactacagac tggaaagaat
      661 caaggaggcc gacaaagaga cctacgtcga gcagcacgag gtggctgtgg ccagatactg
      721 cgacctccct agcaaactgg ggcacaagct taattccgga ctcagatctc
//
