LOCUS       sequence-172573-        8355 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral expression of human full length CSF-1(256).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8355)
  AUTHORS   Heard JM, Roussel MF, Rettenmier CW, Sherr CJ
  TITLE     Synthesis, post-translational processing, and autocrine transforming
            activity of a carboxylterminal truncated form of colony stimulating 
            factor-1.
  JOURNAL   Oncogene Res. 1987 Sep-Oct;1(4):423-40.
  PUBMED    2966924
REFERENCE   2  (bases 1 to 8355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene 
            Res. 1987 Sep-Oct"; volume: "1(4)"; pages: "423-40"
COMMENT     Sequence Label: MSCV-(pbabe puro mcs)-human CSF-1 full length
            ...
FEATURES             Location/Qualifiers
     source          1..8355
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1710..2259
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1854..1871)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2081..2100
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(2262..2264,2265..2267,2268..2981)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2262..2264,2265..2267,2268..2978)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2307..2328)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2568..2587)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2915..2936
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             3143..3657
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    3819..3835
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3843..3849,3850..3867,3868..3873))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3888..3909
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4026..4043)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4197..4785)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4277..4296)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4956..5747,5748..5816))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5579..5598
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5817..5921)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5989..6007
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6045..6067)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6167..6186
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6380..6402
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6530..6549
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             6749..7265
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    7329..7670
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             7737..8153
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     8083..8105
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     8121..8137
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 atgaccgcgc cgggcgccgc cgggcgctgc cctcccacga catggctggg ctccctgctg
       61 ttgttggtct gtctcctggc gagcaggagt atcaccgagg aggtgtcgga gtactgtagc
      121 cacatgattg ggagtggaca cctgcagtct ctgcagcggc tgattgacag tcagatggag
      181 acctcgtgcc aaattacatt tgagtttgta gaccaggaac agttgaaaga tccagtgtgc
      241 taccttaaga aggcatttct cctggtacaa gacataatgg aggacaccat gcgcttcaga
      301 gataacaccc ccaatgccat cgccattgtg cagctgcagg aactctcttt gaggctgaag
      361 agctgcttca ccaaggatta tgaagagcat gacaaggcct gcgtccgaac tttctatgag
      421 acacctctcc agttgctgga gaaggtcaag aatgtcttta atgaaacaaa gaatctcctt
      481 gacaaggact ggaatatttt cagcaagaac tgcaacaaca gctttgctga atgctccagc
      541 caagatgtgg tgaccaagcc tgattgcaac tgcctgtacc ccaaagccat ccctagcagt
      601 gacccggcct ctgtctcccc tcatcagccc ctcgccccct ccatggcccc tgtggctggc
      661 ttgacctggg aggactctga gggaactgag ggcagctccc tcttgcctgg tgagcagccc
      721 ctgcacacag tggatccagg cagtgccaag cagcggccac ccaggagcac ctgccagagc
      781 tttgagccgc cagagacccc agttgtcaag gacagcacca tcggtggctc accacagcct
      841 cgcccctctg tcggggcctt caaccccggg atggaggata ttcttgactc tgcaatgggc
      901 actaattggg tcccagaaga agcctctgga gaggccagtg agattcccgt accccaaggg
      961 acagagcttt ccccctccag gccaggaggg ggcagcatgc agacagagcc cgccagaccc
     1021 agcaacttcc tctcagcatc ttctccactc cctgcatcag caaagggcca acagccggca
     1081 gatgtaactg gtacagcctt gcccagggtg ggccccgtga ggcccactgg ccaggactgg
     1141 aatcacaccc cccagaagac agaccatcca tctgccctgc tcagagaccc cccggagcca
     1201 ggctctccca ggatctcatc actgcgcccc cagggcctca gcaacccctc caccctctct
     1261 gctcagccac agctttccag aagccactcc tcgggcagcg tgctgcccct tggggagctg
     1321 gagggcagga ggagcaccag ggatcggagg agccccgcag agccagaagg aggaccagca
     1381 agtgaagggg cagccaggcc cctgccccgt tttaactccg ttcctttgac tgacacaggc
     1441 catgagaggc agtccgaggg atcctccagc ccgcagctcc aggagtctgt cttccacctg
     1501 ctggtgccca gtgtcatcct ggtcttgctg gccgtcggag gcctcttgtt ctacaggtgg
     1561 aggcggcgga gccatcaaga gcctcagaga gcggattctc ccttggagca accagagggc
     1621 agccccctga ctcaggatga cagacaggtg gaactgccag tgtagaggga attcagatct
     1681 cgagcgggat caattccgcc ccccccctaa cgttactggc cgaagccgct tggaataagg
     1741 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     1801 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     1861 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     1921 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     1981 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     2041 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     2101 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     2161 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
     2221 ccacggggac gtggttttcc tttgaaaaac acgataatac catggtgagc aagggcgagg
     2281 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     2341 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     2401 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     2461 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     2521 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     2581 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     2641 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     2701 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     2761 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     2821 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     2881 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     2941 ccgccgggat cactctcggc atggacgagc tgtacaagta aagcggccgc gactctagag
     3001 tcgacctgca ggcatgcaag cttcaggtag ccggctaacg ttaacaaccg gtacctctag
     3061 aactatagct agcatgcgca aatttaaagc gctgatatcg ataaaataaa agattttatt
     3121 tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctta
     3181 agtaacgcca ttttgcaagg catggaaaat acataactga gaatagagaa gttcagatca
     3241 aggttaggaa cagagagaca gcagaatatg ggccaaacag gatatctgtg gtaagcagtt
     3301 cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtcccgc cctcagcagt
     3361 ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct
     3421 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag
     3481 ctcaataaaa gagcccacaa cccctcactc ggcgcgccag tcctccgata gactgcgtcg
     3541 cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct
     3601 gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatggg
     3661 taacagtttc ttgaagttgg agaacaacat tctgagggta ggagtcgaat attaagtaat
     3721 cctgactcaa ttagccactg ttttgaatcc acatactcca atactcctga aatagttcat
     3781 tatggacagc gcagaaagag ctggggagaa ttgtgaaatt gttatccgct cacaattcca
     3841 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     3901 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     3961 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     4021 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     4081 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     4141 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     4201 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     4261 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     4321 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     4381 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     4441 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     4501 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     4561 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     4621 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     4681 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     4741 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     4801 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     4861 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     4921 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     4981 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     5041 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     5101 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     5161 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     5221 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     5281 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     5341 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     5401 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     5461 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     5521 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     5581 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     5641 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     5701 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     5761 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     5821 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     5881 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     5941 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     6001 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     6061 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     6121 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     6181 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     6241 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     6301 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     6361 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggcgca aggaatggtg
     6421 catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc
     6481 cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg
     6541 cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg
     6601 cgtagaggcg attagtccaa tttgttaaag acaggatatc agtggtccag gctctagttt
     6661 tgactcaaca atatcaccag ctgaagccta tagagtacga gccatagata aaataaaaga
     6721 ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc
     6781 tagcttaagt aacgccattt tgcaaggcat ggaaaataca taactgagaa tagagaagtt
     6841 cagatcaagg ttaggaacag agagacagca gaatatgggc caaacaggat atctgtggta
     6901 agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg gtcccgccct
     6961 cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc
     7021 tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct
     7081 ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc tccgatagac
     7141 tgcgtcgccc gggtacccgt attcccaata aagcctcttg ctgtttgcat ccgaatcgtg
     7201 gactcgctga tccttgggag ggtctcctca gattgattga ctgcccacct cgggggtctt
     7261 tcatttggag gttccaccga gatttggaga cccctgccca gggaccaccg acccccccgc
     7321 cgggaggtaa gctggccagc ggtcgtttcg tgtctgtctc tgtctttgtg cgtgtttgtg
     7381 ccggcatcta atgtttgcgc ctgcgtctgt actagttagc taactagctc tgtatctggc
     7441 ggacccgtgg tggaactgac gagttctgaa cacccggccg caaccctggg agacgtccca
     7501 gggactttgg gggccgtttt tgtggcccga cctgaggaag ggagtcgatg tggaatccga
     7561 ccccgtcagg atatgtggtt ctggtaggag acgagaacct aaaacagttc ccgcctccgt
     7621 ctgaattttt gctttcggtt tggaaccgaa gccgcgcgtc ttgtctgctg cagcgctgca
     7681 gcatcgttct gtgttgtctc tgtctgactg tgtttctgta tttgtctgaa aattagggcc
     7741 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg
     7801 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat
     7861 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc
     7921 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca
     7981 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc
     8041 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt
     8101 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gccggaattg
     8161 ggatcccagt gtggtggtac gtaggaattc ggggggaaag tgaaagtttg cctgggtcct
     8221 ctcggcgcca gagccgctct ccgcatccca ggacagcggt gcggccctcg gccggggcgc
     8281 ccactccgca gcagccagcg agcgagcgag cgagcgaggg cggccgacgc gcccggccgg
     8341 gacccagctg cccgt
//
