LOCUS       sequence-172574-       11569 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  target  miRNA830*  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11569)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 11569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941-PacI-ath-STTM830_
FEATURES             Location/Qualifiers
     source          1..11569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             126..1199
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      1265..1459
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(1695..1717)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1803..1943
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1858..1877
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1958..1975)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2129..2717)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2804..3598)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    4023..4047
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(4133..4385)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(4395..4946)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(4952..5332)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     primer_bind     5620..5639
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5620..5638
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        6046..6391
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     6334..6354
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     polyA_signal    6554..6730
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(7292..7310)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7293..7310)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     7949..7968
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(8071..8076,8077..8093,8094..8099))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      8361..9068
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(9093..9110)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9093..9109)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9102..9124)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    9312..9336
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10637..11266
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc
       61 gtaagcggct gggttgcctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa
      121 tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg
      181 acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag
      241 cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac
      301 cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt
      361 ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg
      421 ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc
      481 cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg
      541 acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga
      601 agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc
      661 ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca
      721 ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat
      781 ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg
      841 agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc
      901 cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc
      961 tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga
     1021 tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc
     1081 tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc
     1141 cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat
     1201 gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct
     1261 ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt
     1321 acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa
     1381 aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa
     1441 cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc
     1501 ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg
     1561 ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg
     1621 cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt
     1681 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     1741 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     1801 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
     1861 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     1921 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     1981 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     2041 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     2101 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     2161 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     2221 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2281 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     2341 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     2401 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2461 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2521 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     2581 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2641 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2701 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     2761 acgaaaactc acgttaaggg attttggtca tgcattctag gtactaaaac aattcatcca
     2821 gtaaaatata atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata
     2881 gctcgacata ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt
     2941 cataccactt gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat
     3001 ctttcacaaa gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg
     3061 gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt
     3121 cccagttttc gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta
     3181 agcggctgtc taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc
     3241 tgatgcactc cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt
     3301 ccgagcaaag gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt
     3361 caaagtgcag gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt
     3421 cccgttccac atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt
     3481 tttcattttc tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta
     3541 cgcagcggta tttttcgatc agttttttca attccggtga tattctcatt ttagccattt
     3601 attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa
     3661 gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt
     3721 ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc
     3781 gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga
     3841 gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac
     3901 atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg
     3961 ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct
     4021 ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg
     4081 cggacgtttt taatgtactg aattaacgcc gaattaattc gagctcggat ctgataattt
     4141 atttgaaaat tcataagaaa agcaaacgtt acatgaattg atgaaacaat acaaagacag
     4201 ataaagccac gcacatttag gatattggcc gagattactg aatattgagt aagatcacgg
     4261 aatttctgac aggagcatgt cttcaattca gcccaaatgg cagttgaaat actcaaaccg
     4321 ccccatatgc aggagcggat cattcattgt ttgtttggtt gcctttgcca acatgggagt
     4381 ccaagattct gcagtcaaat ctcggtgacg ggcaggaccg gacggggcgg taccggcagg
     4441 ctgaagtcca gctgccagaa acccacgtca tgccagttcc cgtgcttgaa gccggccgcc
     4501 cgcagcatgc cgcggggggc atatccgagc gcctcgtgca tgcgcacgct cgggtcgttg
     4561 ggcagcccga tgacagcgac cacgctcttg aagccctgtg cctccaggga cttcagcagg
     4621 tgggtgtaga gcgtggagcc cagtcccgtc cgctggtggc ggggggagac gtacacggtc
     4681 gactcggccg tccagtcgta ggcgttgcgt gccttccagg ggcccgcgta ggcgatgccg
     4741 gcgacctcgc cgtccacctc ggcgacgagc cagggatagc gctcccgcag acggacgagg
     4801 tcgtccgtcc actcctgcgg ttcctgcggc tcggtacgga agttgaccgt gcttgtctcg
     4861 atgtagtggt tgacgatggt gcagaccgcc ggcatgtccg cctcggtggc acggcggatg
     4921 tcggccgggc gtcgttctgg gctcatcgat tcgatttggt gtatcgagat tggttatgaa
     4981 attcagatgc tagtgtaatg tattggtaat ttgggaagat ataataggaa gcaaggctat
     5041 ttatccattt ctgaaaaggc gaaatggcgt caccgcgagc gtcacgcgca ttccgttctt
     5101 gctgtaaagc gttgtttggt acacttttga ctagcgaggc ttggcgtgtc agcgtatcta
     5161 ttcaaaagtc gttaatggct gcggatcaag aaaaagttgg aatagaaaca gaatacccgc
     5221 gaaattcagg cccggttgcc atgtcctaca cgccgaaata aacgaccaaa ttagtagaaa
     5281 aataaaaact gactcggata cttacgtcac gtcttgcgca ctgatttgaa aaatctcaga
     5341 attcatggat cctaggtgag tctagagagt taattaagaa aggcggacag gtatcccgta
     5401 atctgctgct tgcaaactta attaagaaag gcggacaggt atccggtaat ctgctgcttg
     5461 caaacttaat taagtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaaggaga
     5521 tcctttggat cacaaaaaga aagcccgctc attaggcggg ttaaattccc gggtactgca
     5581 gccgattcat taatgcaggt taacctggct tatcgaaatt aatacgactc actataggga
     5641 gaccggcaga tctgacccct actccaaaaa tgtcaaagat acagtctcag aagaccaaag
     5701 ggctattgag acttttcaac aaagggtaat ttcgggaaac ctcctcggat tccattgccc
     5761 agctatctgt cacttcatcg aaaggacagt ggaaaaggaa ggtggctcct acaaatgcca
     5821 tcattgcgat aaaggaaagg ctatcattca agatgcctct gccgacagtg gtcccaaaga
     5881 tggaccccca cccacgagga gcatcgtggg aaaagaagac gttccaacca cgtcttcaaa
     5941 gcaagtggat tgatgtgaca tctccactga cgtaagggat gacgcacaat cccaccccta
     6001 ctccaaaaat gtcaaagata ctgtctcaga agaccaaagg gctattgaga cttttcaaca
     6061 aagggtaatt tcgggaaacc tcctcggatt ccattgccca gctatctgtc acctcatcga
     6121 aaggacagta gaaaaggaag gtggctccta caaatgccat cattgcgatg aaggaaaggc
     6181 tatcattcaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag
     6241 catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgacat
     6301 ctccactgac ataagggatg acgcacaacc ccactatcct tcgcaagacc cttcctctat
     6361 ataaggaagt tcatttcatt tggagaggac agcccaagct taacctaaac tatctattgg
     6421 agaagagttg ttgttgttat ggtctaattt aaatatggtc taaagaagaa gaataaccta
     6481 aactatctat tggagaagag aattcggtac gctgaaatca ccagtctctc tctacaaatc
     6541 tatctctctc tattttctcc ataaataatg tgtgagtagt ttcccgataa gggaaattag
     6601 ggttcttata gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt
     6661 atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat ccagtactaa
     6721 aatccagatc tcctaaagtc cctatagatc tttgtcgtga atataaacca gacacgagac
     6781 gactagacct ggagcccaga cgccgttcga agctggaagt accgcttagg caggaggccg
     6841 ttagggaaaa gatgctaagg cagggttggt tacgttgact cccccgtagg tttggtttaa
     6901 atatgatgaa gtggacggaa ggaaggagga agacaaggaa ggataaggtt gcaggccctg
     6961 tgcaaggtaa gaagatggaa atttgataga ggtacgctac tatacttata ctatacgcta
     7021 agggaatgat tgtatttata ccctataccc cctaataacc ccttatcaat ttaagaaata
     7081 atccgcataa gcccccgctt aaaaattggt atcagagcca tgaataggtc tatgaccaaa
     7141 actcaagagg ataaaacctc accaaaatac gaaagagtcc ttaactctaa agataaaaga
     7201 tctttcaaga tcaaaactag ttcccccaca ccggtgacgg ggaccgcatg cgacctctag
     7261 accatcacat tggagcttca gctgctcgag ttctatagtg tcacctaaat cgtatgtgta
     7321 tgatacataa ggttatgtat taattgtagc cgcgttctta cgtcgacgta ccaattacgc
     7381 cccgccctgt cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
     7441 gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
     7501 cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
     7561 taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat
     7621 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat
     7681 gtgtagaaac cgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
     7741 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catattacca gctcaccgtc
     7801 tttcattgcc atacggaact ccggatgagc attcatcagg cgggcaagaa tgtgaataaa
     7861 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
     7921 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
     7981 acgatgccat tgggatatat caacggtggt atatccagtg atttctttct ccattttagc
     8041 ttccttagct cctgaaaatc tcgataactc attaaagctt atcgatgata agctgtcaaa
     8101 catgagaatt tagcccgcct aatgagcggg ctttttgttg tgatccgcgt tgctggcgtt
     8161 tttccatagg ctccgccccc ctgacgagca tcacaaaaat agacgctcaa gtcagaggtg
     8221 gcgaaacccg acaggactat aaagatacca ggcgtttccc ccctggaagc tccctcgtgc
     8281 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctt aattaagacc
     8341 cgggactagt ccctagagtc ctgctttaat gagatatgcg agacgcctat gatcgcatga
     8401 tatttgcttt caattctgtt gtgcacgttg taaaaaacct gagcatgtgt agctcagatc
     8461 cttaccgccg gtttcggttc attctaatga atatatcacc cgttactatc gtatttttat
     8521 gaataatatt ctccgttcaa tttactgatt gtaccctact acttatatgt acaatattaa
     8581 aatgaaaaca atatattgtg ctgaataggt ttatagcgac atctatgata gagcgccaca
     8641 ataacaaaca attgcgtttt attattacaa atccaatttt aaaaaaagcg gcagaaccgg
     8701 tcaaacctaa aagactgatt acataaatct tattcaaatt tcaaaagtgc cccaggggct
     8761 agtatctacg acacaccgag cggcgaacta ataacgctca ctgaagggaa ctccggttcc
     8821 ccgccggcgc gcatgggtga gattccttga agttgagtat tggccgtccg ctctaccgaa
     8881 agttacgggc accattcaac ccggtccagc acggcggccg ggtaaccgac ttgctgcccc
     8941 gagaattatg cagcattttt ttggtgtatg tgggccccaa atgaagtgca ggtcaaacct
     9001 tgacagtgac gacaaatcgt tgggcgggtc cagggcgaat tttgcgacaa catgtcgagg
     9061 ctcagcagga cctgcaggca tgcaagcttg gcactggccg tcgttttaca acgtcgtgac
     9121 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     9181 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     9241 ggcgaatgct agagcagctt gagcttggat cagattgtcg tttcccgcct tcagtttaaa
     9301 ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag
     9361 aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca
     9421 tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct ccgctgctat
     9481 agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag
     9541 tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt
     9601 tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag
     9661 agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc
     9721 aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc
     9781 ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac
     9841 gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt
     9901 gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac
     9961 accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag
    10021 cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg
    10081 aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc
    10141 gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc
    10201 ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt
    10261 gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc
    10321 caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg
    10381 aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct
    10441 caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc
    10501 cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg
    10561 agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa
    10621 tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa
    10681 gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt
    10741 agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc
    10801 gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg
    10861 gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat
    10921 caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac
    10981 cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc
    11041 ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc
    11101 gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc
    11161 aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg
    11221 cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa
    11281 gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc
    11341 aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag
    11401 ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt
    11461 accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat
    11521 gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaa
//
