LOCUS       sequence-172576-        1668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Toxin-antitoxin stabilized bioluminescent reporter plasmid for 
            Streptococcus expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Loh JM, Proft T
  TITLE     Toxin-antitoxin-stabilized reporter plasmids for biophotonic imaging
            of Group A streptococcus.
  JOURNAL   Appl Microbiol Biotechnol. 2013 Nov;97(22):9737-45. doi: 
            10.1007/s00253-013-5200-7. Epub 2013 Sep 6.
  PUBMED    24061415
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-013-5200-7"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2013-11"; volume: "97"; issue: "22"; pages: 
            "9737-45"
COMMENT     Sequence Label: pLZ12Km2-P23R:TA:ffluc
FEATURES             Location/Qualifiers
     source          1..1668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..1668
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(82..100)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 aaggaggaat aaaaaatgga agacgccaaa aacataaaga aaggcccggc gccattctat
       61 cctctagagg atggaaccgc tggagagcaa ctgcataagg ctatgaagag atacgccctg
      121 gttcctggaa caattgcttt tacagatgca catatcgagg tgaacatcac gtacgcggaa
      181 tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct gaatacaaat
      241 cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt gttgggcgcg
      301 ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg tgaattgctc
      361 aacagtatga acatttcgca gcctaccgta gtgtttgttt ccaaaaaggg gttgcaaaaa
      421 attttgaacg tgcaaaaaaa attaccaata atccagaaaa ttattatcat ggattctaaa
      481 acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct acctcccggt
      541 tttaatgaat acgattttgt accagagtcc tttgatcgtg acaaaacaat tgcactgata
      601 atgaattcct ctggatctac tgggttacct aagggtgtgg cccttccgca tagaactgcc
      661 tgcgtcagat tctcgcatgc cagagatcct atttttggca atcaaatcat tccggatact
      721 gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac actcggatat
      781 ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct gtttttacga
      841 tcccttcagg attacaaaat tcaaagtgcg ttgctagtac caaccctatt ttcattcttc
      901 gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat tgcttctggg
      961 ggcgcacctc tttcgaaaga agtcggggaa gcggttgcaa aacgcttcca tcttccaggg
     1021 atacgacaag gatatgggct cactgagact acatcagcta ttctgattac acccgagggg
     1081 gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat
     1141 ctggataccg ggaaaacgct gggcgttaat cagagaggcg aattatgtgt cagaggacct
     1201 atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat tgacaaggat
     1261 ggatggctac attctggaga catagcttac tgggacgaag acgaacactt cttcatagtt
     1321 gaccgcttga agtctttaat taaatacaaa ggatatcagg tggcccccgc tgaattggaa
     1381 tcgatattgt tacaacaccc caacatcttc gacgcgggcg tggcaggtct tcccgacgat
     1441 gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac gatgacggaa
     1501 aaagagatcg tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga
     1561 gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc aagaaaaatc
     1621 agagagatcc tcataaaggc caagaagggc ggaaagtcca aattgtaa
//
