LOCUS       sequence-172578-        4618 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Toxin-antitoxin stabilized bioluminescent reporter plasmid for 
            Streptococcus expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Loh JM, Proft T
  TITLE     Toxin-antitoxin-stabilized reporter plasmids for biophotonic imaging
            of Group A streptococcus.
  JOURNAL   Appl Microbiol Biotechnol. 2013 Nov;97(22):9737-45. doi: 
            10.1007/s00253-013-5200-7. Epub 2013 Sep 6.
  PUBMED    24061415
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-013-5200-7"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2013-11"; volume: "97"; issue: "22"; pages: 
            "9737-45"
COMMENT     Sequence Label: pLZ12Km2-P23R:TA:fflucRT
FEATURES             Location/Qualifiers
     source          1..4618
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     472..494
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     486..503
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     487..503
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     513..532
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        513..531
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     742..758
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     742..758
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(792..808)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(793..809)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             complement(1631..1642)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 aagttttggg attgttaagg gttccgaggc tcaacgtcaa taaagcaatt ggaataaaga
       61 agcgaaaaag gagaagtcgg ttcagaaaaa gaaggatatg gatctggagc tgtaatataa
      121 aaaccttctt caactaacgg ggcaggttag tgacattaga aaaccgactg taaaaagtac
      181 agtcggcatt atctcatatt ataaaagcca gtcattaggc ctatctgaca attcctgaat
      241 agagttcata aacaatcctg catgataacc atcacaaaca gaatgatgta cctgtaaaga
      301 tagcggtaaa tatattgaat tacctttatt aatgaatttt cctgctgtaa taatgggtag
      361 aaggtaatta ctattattat tgatatttaa gttaaaccca gtaaatgaag tccatgctgg
      421 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
      481 gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag ggcgaattgg
      541 agctctcgaa aagccctgac aaccctcgtt cctaaaaagg aataagcgtt tggtcagtaa
      601 ataatagaaa taaaaaatca gacctaagac tgatgacaaa aagagcaaat tttgataaaa
      661 tagtattaga attaaattaa aaagggaggc caaatataat gaaaaatatg aatgacaatg
      721 atgttccgcg gtggcggccg ctctagaact agtggatccc ccgggctgca ggaattcgat
      781 atcaagctta tcgataccgt cgacctcgag ggggggccca tcagtctgac gaccaagaga
      841 gccataaaca ccaatagcct taacatcatc cccatattta tccaatattc gttccttaat
      901 ttcatgaaca atcttcattc tttcttctct agtcattatt attggtccat tcactattct
      961 cattcccttt tcagataatt ttagatttgc ttttctaaat aagaatattt ggagagcacc
     1021 gttcttattc agctattaat aactcgtctt cctaagcatc cttcaatcct tttaataaca
     1081 attatagcat ctaatcttca acaaactggc ccgtttgttg aactactctt taataaaata
     1141 atttttccgt tcccaattcc acattgcaat aatagaaaat ccatcttcat cggctttttc
     1201 gtcatcatct gtatgaatca aatcgccttc ttctgtgtca tcaaggttta attttttatg
     1261 tatttctttt aacaaaccac cataggagat taacctttta cggtgtaaac cttcctccaa
     1321 atcagacaaa cgtttcaaat tcttttcttc atcatcggtc ataaaatccg tatcctttac
     1381 aggatatttt gcagtttcgt caattgccga ttgtatatcc gatttatatt tatttttcgg
     1441 tcgatttttt attaaaacgt ctcaaaatcg tttctgagac gttttagcgt ttatttcgtt
     1501 tagttatcgg cataatcgtt aaaacaggcg ttatcgtagc gtaaaagccc ttgagcgtag
     1561 cgtggctttg cagcgaagat gttgtctgtt agattatgaa agccgatgac tgaatgaaat
     1621 aataagcgca gcgtccttct atttcggttg gaggaggctc aagggagttt gagggaatga
     1681 aattccctca tgggtttgat tttaaaaatt gcttgcaatt ttgccgagcg gtagcgctgg
     1741 aaaatttttg aaaaaaattt ggaatttgga aaaaaatggg ggggaaagga agcgaatttt
     1801 gcttccgtac tacgaccccc cattaagtgc cgagtgccaa tttttgtgcc aaaaacgctc
     1861 tatcccaact ggctcaaggg tttgaggggt ttttcaatcg ccaacgaatc gccaacgttt
     1921 tcgccaacgt tttttataaa tctatattta agtagcttta ttgttgtttt tatgattaca
     1981 aagtgataca ctaattttat aaaattattt gattggagtt ttttaaatgg tgatttcaga
     2041 atcgaaaaaa agagttatga tttctctgac aaaagagcaa gataaaaaat taacagatat
     2101 ggcgaaacaa aaaggttttt caaaatctgc ggttgcggcg ttagctatag aagaatatgc
     2161 aagaaaggaa tcagaacaaa aaaaataagc gaaagctcgc gtttttagaa ggatacgagt
     2221 tttcgctact tgtttttgat aaggtaatta tatcatggct attaaaaata ctaaagctag
     2281 aaattttgga tttttattat atcctgactc aattcctaat gattggaaag aaaaattaga
     2341 gagtttgggc gtatctatgg ctgtcagtcc tttacacgat atggacgaaa aaaaagataa
     2401 agatacatgg aatagtagtg atgttatacg aaatggaaag cactataaaa aaccacacta
     2461 tcacgttata tatatagcac gaaatcctgt acaatagaaa gcgttaggaa ccagattaag
     2521 cgaaaattgg ggaatagttc agttgctcat gttgagatac ttgattatat caaaggttca
     2581 tatgaatatt tgactcatga atcaaaggac gctattgcta agaataaaca tatatacgac
     2641 aaaaaagata ttttgaacat taatgatttt gatattgacc gctatataac acttgatgaa
     2701 agccaaaaaa gaaaattgaa gaatttactt ttagatatag tggatgacta taatttggta
     2761 aatacaaaag atttaatggc ttttattcgc cttaggggag cggagtttgg aattttaaat
     2821 acgaatgatg taaaagatat tgtttcaaca aactctagcg cctttagatt atggtttgag
     2881 ggcaattatc agtgtggata tagagcaagt tatgcaaagg ttcttgatgc tgaaacgggg
     2941 gaaataaaat gacaaacaaa gaaaaagagt tatttgctga aaatgaggaa ttaaaaaaag
     3001 aaattaagga cttaaaagag cgtattgaaa gatacagaga aatggaagtt gaattaagta
     3061 caacaataga tttattgaga ggagggatta ttgaataaat aaaagccccc ctgacgaaag
     3121 tcgacgatac aaattcctcg taggcgctcg ggacccctat ctagcgaact tttagaaaag
     3181 atataaaaca tcagagtatg gacagttgcg gatgtacttc agaaaagatt agatgtctaa
     3241 aaagcttgta gttaaagctt tttagacatc taaatctagg tactaaaaca attcatccag
     3301 taaaatataa tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag
     3361 ctcgacatac tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc
     3421 ataccacttg tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc
     3481 tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg
     3541 cttttccgtc tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc
     3601 ccagttttcg caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa
     3661 gcggctgtct aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct
     3721 gatgcactcc gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc
     3781 cgagcaaagg acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc
     3841 aaagtgcagg acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc
     3901 ccgttccaca tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt
     3961 ttcattttct cccaccagct tatatacctt agcaggagac attcctttcc gtatctttta
     4021 cgcagcggta tttttcgatc agttttttca attccggtga tattctcatt ttagccattt
     4081 attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa
     4141 gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt
     4201 ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc
     4261 acaattatga tagaatttac aagctataag gttattgtcc tgggtttcaa gcattagtcc
     4321 atgcaagttt ttatgctttg cccattctat agatatattg ataagcgcgc tgcctatgcc
     4381 ttgccccctg aaatccttac atacggcgat atcttctata taaaagatat attatcttat
     4441 cagtattgtc aatatattca aggcaatctg cctcctcatc ctcttcatcc tcttcgtctt
     4501 ggtagctttt taaatatggc gcttcataga gtaattctgt aaaggtccaa ttctcgtttt
     4561 catacctcgg tataatctta cctatcacct caaatggttc gctgggttta tcgtcgac
//
