LOCUS       sequence-172579-        5650 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Toxin-antitoxin stabilized bioluminescent reporter plasmid for 
            Streptococcus expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5650)
  AUTHORS   Loh JM, Proft T
  TITLE     Toxin-antitoxin-stabilized reporter plasmids for biophotonic imaging
            of Group A streptococcus.
  JOURNAL   Appl Microbiol Biotechnol. 2013 Nov;97(22):9737-45. doi: 
            10.1007/s00253-013-5200-7. Epub 2013 Sep 6.
  PUBMED    24061415
REFERENCE   2  (bases 1 to 5650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-013-5200-7"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2013-11"; volume: "97"; issue: "22"; pages: 
            "9737-45"
COMMENT     Sequence Label: pLZ12Km2-P23R:TA:Xen5
FEATURES             Location/Qualifiers
     source          1..5650
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             22..1104
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             1124..2107
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVAIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMKYT"
     CDS             2132..3574
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPESDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MRHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             3594..4517
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PDNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             4537..5649
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
ORIGIN
        1 gaattcaagg aggaataaaa aatgaaattt ggaaactttt tgcttacata ccaacctccc
       61 caattttctc aaacagaggt aatgaaacgt ttggttaaat taggtcgcat ctctgaggag
      121 tgtggttttg ataccgtatg gttactggag catcatttca cggagtttgg tttgcttggt
      181 aacccttatg tcgctgctgc atatttactt ggcgcgacta aaaaattgaa tgtaggaact
      241 gccgctattg ttcttcccac agcccatcca gtacgccaac ttgaagatgt gaatttattg
      301 gatcaaatgt caaaaggacg atttcggttt ggtatttgcc gagggcttta caacaaggac
      361 tttcgcgtat tcggcacaga tatgaataac agtcgcgcct tagcggaatg ctggtacggg
      421 ctgataaaga atggcatgac agagggatat atggaagctg ataatgaaca tatcaagttc
      481 cataaggtaa aagtaaaccc cgcggcgtat agcagaggtg gcgcaccggt ttatgtggtg
      541 gctgaatcag cttcgacgac tgagtgggct gctcaatttg gcctaccgat gatattaagt
      601 tggattataa atactaacga aaagaaagca caacttgagc tttataatga agtggctcaa
      661 gaatatgggc acgatattca taatatcgac cattgcttat catatataac atctgtagat
      721 catgactcaa ttaaagcgaa agagatttgc cggaaatttc tggggcattg gtatgattct
      781 tatgtgaatg ctacgactat ttttgatgat tcagaccaaa caagaggtta tgatttcaat
      841 aaagggcagt ggcgtgactt tgtattaaaa ggacataaag atactaatcg ccgtattgat
      901 tacagttacg aaatcaatcc cgtgggaacg ccgcaggaat gtattgacat aattcaaaaa
      961 gacattgatg ctacaggaat atcaaatatt tgttgtggat ttgaagctaa tggaacagta
     1021 gacgaaatta ttgcttccat gaagctcttc cagtctgatg tcatgccatt tcttaaagaa
     1081 aaacaacgtt cgctattata ttagtcgagg aggagagaaa gaaatgaaat ttggattgtt
     1141 cttccttaac ttcatcaatt caacaactgt tcaagaacaa agtatagttc gcatgcagga
     1201 aataacggag tatgttgata agttgaattt tgaacagatt ttagtgtatg aaaatcattt
     1261 ttcagataat ggtgttgtcg gcgctcctct gactgtttct ggttttctgc tcggtttaac
     1321 agagaaaatt aaaattggtt cattaaatca catcattaca actcatcatc ctgtcgccat
     1381 agcggaggaa gcttgcttat tggatcagtt aagtgaaggg agatttattt tagggtttag
     1441 tgattgcgaa aaaaaagatg aaatgcattt ttttaatcgc ccggttgaat atcaacagca
     1501 actatttgaa gagtgttatg aaatcattaa cgatgcttta acaacaggct attgtaatcc
     1561 agataacgat ttttatagct tccctaaaat atctgtaaat ccccatgctt atacgccagg
     1621 cggacctcgg aaatatgtaa cagcaaccag tcatcatatt gttgagtggg cggccaaaaa
     1681 aggtattcct ctcatcttta agtgggatga ttctaatgat gttagatatg aatatgctga
     1741 aagatataaa gccgttgcgg ataaatatga cgttgaccta tcagagatag accatcagtt
     1801 aatgatatta gttaactata acgaagatag taataaagct aaacaagaga cgcgtgcatt
     1861 tattagtgat tatgttcttg aaatgcaccc taatgaaaat ttcgaaaata aacttgaaga
     1921 aataattgca gaaaacgctg tcggaaatta tacggagtgt ataactgcgg ctaagttggc
     1981 aattgaaaag tgtggtgcga aaagtgtatt gctgtccttt gaaccaatga atgatttgat
     2041 gagccaaaaa aatgtaatca atattgttga tgataatatt aagaagtacc acatgaaata
     2101 tacctaagtc gactcgagga ggatggcaaa tatgactaaa aaaatttcat tcattattaa
     2161 cggccaggtt gaaatctttc ccgaaagtga tgatttagtg caatccatta attttggtga
     2221 taatagtgtt tacctgccaa tattgaatga ctctcatgta aaaaacatta ttgattgtaa
     2281 tggaaataac gaattacggt tgcataacat tgtcaatttt ctctatacgg tagggcaaag
     2341 atggaaaaat gaagaatact caagacgcag gacatacatt cgtgacttaa aaaaatatat
     2401 gggatattca gaagaaatgg ctaagctaga ggccaattgg atatctatga ttttatgttc
     2461 taaaggcggc ctttatgatg ttgtagaaaa tgaacttggt tctcgccata tcatggatga
     2521 atggctacct caggatgaaa gttatgttcg ggcttttccg aaaggtaaat ctgtacatct
     2581 gttggcaggt aatgttccat tatctgggat catgtctata ttacgcgcaa ttttaactaa
     2641 gaatcagtgt attataaaaa catcgtcaac cgatcctttt accgctaatg cattagcgtt
     2701 aagttttatt gatgtagacc ctaatcatcc gataacgcgc tctttatctg ttatatattg
     2761 gccccaccaa ggtgatacat cactcgcaaa agaaattatg cgacatgcgg atgttattgt
     2821 cgcttgggga gggccagatg cgattaattg ggcggtagag catgcgccat cttatgctga
     2881 tgtgattaaa tttggttcta aaaagagtct ttgcattatc gataatcctg ttgatttgac
     2941 gtccgcagcg acaggtgcgg ctcatgatgt ttgtttttac gatcagcgag cttgtttttc
     3001 tgcccaaaac atatattaca tgggaaatca ttatgaggaa tttaagttag cgttgataga
     3061 aaaacttaat ctatatgcgc atatattacc gaatgccaaa aaagattttg atgaaaaggc
     3121 ggcctattct ttagttcaaa aagaaagctt gtttgctgga ttaaaagtag aggtggatat
     3181 tcatcaacgt tggatgatta ttgagtcaaa tgcaggtgtg gaatttaatc aaccacttgg
     3241 cagatgtgtg taccttcatc acgtcgataa tattgagcaa atattgcctt atgttcaaaa
     3301 aaataagacg caaaccatat ctatttttcc ttgggagtca tcatttaaat atcgagatgc
     3361 gttagcatta aaaggtgcgg aaaggattgt agaagcagga atgaataaca tatttcgagt
     3421 tggtggatct catgacggaa tgagaccgtt gcaacgatta gtgacatata tttctcatga
     3481 aaggccatct aactatacgg ctaaggatgt tgcggttgaa atagaacaga ctcgattcct
     3541 ggaagaagat aagttccttg tatttgtccc ataagtcgag gaggagtaaa agtatggaaa
     3601 atgaatcaaa atataaaacc atcgaccacg ttatttgtgt tgaaggaaat aaaaaaattc
     3661 atgtttggga aacgctgcca gaagaaaaca gcccaaagag aaagaatgcc attattattg
     3721 cgtctggttt tgcccgcagg atggatcatt ttgctggtct ggcggaatat ttatcgcgga
     3781 atggatttca tgtgatccgc tatgattcgc ttcaccacgt tggattgagt tcagggacaa
     3841 ttgatgaatt tacaatgtct ataggaaagc agagcttgtt agcagtggtt gattggttaa
     3901 ctacacgaaa aataaataac ttcggtatgt tggcttcaag cttatctgcg cggatagctt
     3961 atgcaagcct atctgaaatc aatgcttcgt ttttaatcac cgcagtcggt gttgttaact
     4021 taagatattc tcttgaaaga gctttagggt ttgattatct cagtctaccc attaatgaat
     4081 tgccggataa tctagatttt gaaggccata aattgggtgc tgaagtcttt gcgagagatt
     4141 gtcttgattt tggttgggaa gatttagctt ctacaattaa taacatgatg tatcttgata
     4201 taccgtttat tgcttttact gcaaataacg ataattgggt caagcaagat gaagttatca
     4261 cattgttatc aaatattcgt agtaatcgat gcaagatata ttctttgtta ggaagttcgc
     4321 atgacttgag tgaaaattta gtggtcctgc gcaattttta tcaatcggtt acgaaagccg
     4381 ctatcgcgat ggataatgat catctggata ttgatgttga tattactgaa ccgtcatttg
     4441 aacatttaac tattgcgaca gtcaatgaac gccgaatgag aattgagatt gaaaatcaag
     4501 caatttctct gtcttaagtc gaggaggaaa acaggtatga cttcatatgt tgataaacaa
     4561 gaaattacag caagctcaga aattgatgat ttgatttttt cgagcgatcc attagtgtgg
     4621 tcttacgacg agcaggaaaa aatcagaaag aaacttgtgc ttgatgcatt tcgtaatcat
     4681 tataaacatt gtcgagaata tcgtcactac tgtcaggcac acaaagtaga tgacaatatt
     4741 acggaaattg atgacatacc tgtattccca acatcggttt ttaagtttac tcgcttatta
     4801 acttctcagg aaaacgagat tgaaagttgg tttaccagta gcggcacgaa tggtttaaaa
     4861 agtcaggtgg cgcgtgacag attaagtatt gagagactct taggctctgt gagttatggc
     4921 atgaaatatg ttggtagttg gtttgatcat caaatagaat tagtcaattt gggaccagat
     4981 agatttaatg ctcataatat ttggtttaaa tatgttatga gtttggtgga attgttatat
     5041 cctacgacat ttaccgtaac agaagaacga atagattttg ttaaaacatt gaatagtctt
     5101 gaacgaataa aaaatcaagg gaaagatctt tgtcttattg gttcgccata ctttatttat
     5161 ttactctgcc attatatgaa agataaaaaa atctcatttt ctggagataa aagcctttat
     5221 atcataaccg gaggcggctg gaaaagttac gaaaaagaat ctctgaaacg tgatgatttc
     5281 aatcatcttt tatttgatac tttcaatctc agtgatatta gtcagatccg agatatattt
     5341 aatcaagttg aactcaacac ttgtttcttt gaggatgaaa tgcagcgtaa acatgttccg
     5401 ccgtgggtat atgcgcgagc gcttgatcct gaaacgttga aacctgtacc tgatggaacg
     5461 ccggggttga tgagttatat ggatgcgtca gcaaccagtt atccagcatt tattgttacc
     5521 gatgatgtcg ggataattag cagagaatat ggtaagtatc ccggcgtgct cgttgaaatt
     5581 ttacgtcgcg tcaatacgag gacgcagaaa gggtgtgctt taagcttaac cgaagcgttt
     5641 gatagttgag
//
