LOCUS       sequence-172581-       11479 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  target  miRNA417  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11479)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 11479)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941-PacI-ath-STTM417
FEATURES             Location/Qualifiers
     source          1..11479
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     polyA_signal    514..690
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1252..1270)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1253..1270)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1909..1928
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2031..2036,2037..2053,2054..2059))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      2322..3029
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(3054..3071)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3054..3070)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3063..3085)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    3273..3297
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4598..5227
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5656..6729
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6795..6989
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7225..7247)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7333..7473
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7388..7407
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7488..7505)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7659..8247)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8334..9128)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9553..9577
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(9663..9915)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(9925..10476)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(10482..10862)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     primer_bind     11054..11073
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11054..11072
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgagactttt caacaaaggg taatttcggg aaacctcctc ggattccatt gcccagctgt
       61 ctgtcacctc atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggctatca ttcaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gacatctcca ctgacataag ggatgacgca caaccccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacagccc aagctttcga
      361 acaaattcga cactaccttc gttgttgttg ttatggtcta atttgccatt taaatatggt
      421 ctaaagaaga agaattcgaa caaattcgac actaccttcg aattcggtac gctgaaatca
      481 ccagtctctc tctacaaatc tatctctctc tattttctcc ataaataatg tgtgagtagt
      541 ttcccgataa gggaaattag ggttcttata gggtttcgct catgtgttga gcatataaga
      601 aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaactt ctaattccta
      661 aaaccaaaat ccagtactaa aatccagatc ccctaaagtc cctatagatc tttgtcgtga
      721 atataaacca gacacgagac gactagacct ggagcccaga cgccgttcga agctagaagt
      781 accgcttagg caggaggccg ttagggaaaa gatgctaagg cagggttggt tacgttgact
      841 cccccgtagg tttggtttaa atatgatgaa gtggacggaa ggaaggagga agacaaggaa
      901 ggataaggtt gcaggccctg cgcaaggtaa gaagatggaa atttgataga ggtacgctac
      961 tatacttata ctatacgcta agggaatgat tgtatttata ccctataccc cctaataacc
     1021 ccttatcaat ttaagaaata atccgcataa gcccccgctt aaaaattggt atcagaacca
     1081 tgaataggtc tatgaccaaa actcaagagg ataaaacctc accaaaatac gaaagagtcc
     1141 ttaactctaa agataaaaga tctttcaaga tcaaaactag ttccctcaca ccggtgacgg
     1201 ggatcgcatg cgacctctag accatcacat tggagcttca gctgctcgag ttctatagtg
     1261 tcacctaaat cgtatgtgta tgatacataa ggttatgtat taattgtagc cgcgttctta
     1321 cgtcgacgta ccaattacgc cccaccctgt cactcatcgc agtactgttg taattcatta
     1381 agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc
     1441 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
     1501 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaat tcacccaggg attggctgag
     1561 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
     1621 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
     1681 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
     1741 catattacca gctcaccgtc tttcattgcc atacggaact ccggatgagc attcatcagg
     1801 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
     1861 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
     1921 aatgcctcaa aatgttctct acgatgccat tgggatatat caacggtggt atatccagtg
     1981 atttctttct ccattttagc ttccttagct cctgaaaatc tcgataactc attaaagctt
     2041 atcgatgata agctgtcaaa catgagaatt tagcccgcct aatgagcggg ctttttgttg
     2101 tgatccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     2161 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     2221 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     2281 cgccttccct taattaagac ccgggactag tccctagagt cctgctttaa tgagatatgc
     2341 gagacgccta tgatcgcatg atatttgctt tcaattctgt tgtgcacgtt gtaaaaaacc
     2401 tgagcatgtg tagctcagat ccttaccgcc ggtttcggtt cattctaatg aatatatcac
     2461 ccgttactat cgtattttta tgaataatat tctccgttca atttactgat tgtaccctac
     2521 tacttatatg tacaatatta aaatgaaaac aatatattgt gctgaatagg tttatagcga
     2581 catctatgat agagcgccac aataacaaac aattgcgttt tattattaca aatccaattt
     2641 taaaaaaagc ggcagaaccg gtcaaaccta aaagactgat tacataaatc ttattcaaat
     2701 ttcaaaagtg ccccaggggc tagtatctac gacacaccga gcggcgaact aataacgctc
     2761 actgaaggga actccggttc cccgccggcg cgcatgggtg agattccttg aagttgagta
     2821 ttggccgtcc gctctaccga aagttacggg caccattcaa cccggtccag cacggcggcc
     2881 gggtaaccga cttgctgccc cgagaattat gcagcatttt tttggtgtat gtgggcccca
     2941 aatgaagtgc aggtcaaacc ttgacagtga cgacaaatcg ttgggcgggt ccagggcgaa
     3001 ttttgcgaca acatgtcgag gctcagcagg acctgcaggc atgcaagctt ggcactggcc
     3061 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     3121 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     3181 caacagttgc gcagcctgaa tggcgaatgc tagagcagct tgagcttgga tcagattgtc
     3241 gtttcccgcc ttcagtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta
     3301 agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc
     3361 gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtactttg
     3421 atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct
     3481 gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc
     3541 tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg
     3601 agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc
     3661 accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag
     3721 ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt
     3781 gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc
     3841 acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc
     3901 ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc
     3961 gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag
     4021 gccgccaagg cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc
     4081 gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg
     4141 cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc
     4201 accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg
     4261 gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg
     4321 acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt
     4381 tcgagccgcc cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg
     4441 atgccaagct ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc
     4501 taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga
     4561 tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa
     4621 ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact
     4681 cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc
     4741 ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg
     4801 cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc
     4861 ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag
     4921 cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt
     4981 cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat
     5041 cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat
     5101 cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga
     5161 acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact
     5221 catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc
     5281 cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc
     5341 atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg
     5401 gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag
     5461 taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga
     5521 aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg
     5581 ttggccaggc gtaagcggct gggttgcctg ccggccctgc aatggcactg gaacccccaa
     5641 gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg
     5701 ctgggtgatg acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc
     5761 gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa
     5821 tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag
     5881 caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc
     5941 atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc
     6001 tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg
     6061 tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac
     6121 cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc
     6181 aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt
     6241 cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg
     6301 gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc
     6361 gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa
     6421 ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc
     6481 ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg
     6541 ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc
     6601 gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg
     6661 caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc
     6721 ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga
     6781 aaaggtctct ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac
     6841 cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg
     6901 actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa
     6961 actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg
     7021 caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct
     7081 atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc
     7141 ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc
     7201 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     7261 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     7321 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt
     7381 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     7441 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
     7501 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     7561 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     7621 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     7681 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     7741 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     7801 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     7861 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     7921 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7981 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     8041 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     8101 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     8161 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     8221 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     8281 gctcagtgga acgaaaactc acgttaaggg attttggtca tgcattctag gtactaaaac
     8341 aattcatcca gtaaaatata atattttatt ttctcccaat caggcttgat ccccagtaag
     8401 tcaaaaaata gctcgacata ctgttcttcc ccgatatcct ccctgatcga ccggacgcag
     8461 aaggcaatgt cataccactt gtccgccctg ccgcttctcc caagatcaat aaagccactt
     8521 actttgccat ctttcacaaa gatgttgctg tctcccaggt cgccgtggga aaagacaagt
     8581 tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga
     8641 gtgtcttctt cccagttttc gcaatccaca tcggccagat cgttattcag taagtaatcc
     8701 aattcggcta agcggctgtc taagctattc gtatagggac aatccgatat gtcgatggag
     8761 tgaaagagcc tgatgcactc cgcatacagc tcgataatct tttcagggct ttgttcatct
     8821 tcatactctt ccgagcaaag gacgccatcg gcctcactca tgagcagatt gctccagcca
     8881 tcatgccgtt caaagtgcag gacctttgga acaggcagct ttccttccag ccatagcatc
     8941 atgtcctttt cccgttccac atcataggtg gtccctttat accggctgtc cgtcattttt
     9001 aaatataggt tttcattttc tcccaccagc ttatatacct tagcaggaga cattccttcc
     9061 gtatctttta cgcagcggta tttttcgatc agttttttca attccggtga tattctcatt
     9121 ttagccattt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta
     9181 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga
     9241 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga
     9301 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta
     9361 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag
     9421 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg
     9481 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg
     9541 ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa
     9601 taacacattg cggacgtttt taatgtactg aattaacgcc gaattaattc gagctcggat
     9661 ctgataattt atttgaaaat tcataagaaa agcaaacgtt acatgaattg atgaaacaat
     9721 acaaagacag ataaagccac gcacatttag gatattggcc gagattactg aatattgagt
     9781 aagatcacgg aatttctgac aggagcatgt cttcaattca gcccaaatgg cagttgaaat
     9841 actcaaaccg ccccatatgc aggagcggat cattcattgt ttgtttggtt gcctttgcca
     9901 acatgggagt ccaagattct gcagtcaaat ctcggtgacg ggcaggaccg gacggggcgg
     9961 taccggcagg ctgaagtcca gctgccagaa acccacgtca tgccagttcc cgtgcttgaa
    10021 gccggccgcc cgcagcatgc cgcggggggc atatccgagc gcctcgtgca tgcgcacgct
    10081 cgggtcgttg ggcagcccga tgacagcgac cacgctcttg aagccctgtg cctccaggga
    10141 cttcagcagg tgggtgtaga gcgtggagcc cagtcccgtc cgctggtggc ggggggagac
    10201 gtacacggtc gactcggccg tccagtcgta ggcgttgcgt gccttccagg ggcccgcgta
    10261 ggcgatgccg gcgacctcgc cgtccacctc ggcgacgagc cagggatagc gctcccgcag
    10321 acggacgagg tcgtccgtcc actcctgcgg ttcctgcggc tcggtacgga agttgaccgt
    10381 gcttgtctcg atgtagtggt tgacgatggt gcagaccgcc ggcatgtccg cctcggtggc
    10441 acggcggatg tcggccgggc gtcgttctgg gctcatcgat tcgatttggt gtatcgagat
    10501 tggttatgaa attcagatgc tagtgtaatg tattggtaat ttgggaagat ataataggaa
    10561 gcaaggctat ttatccattt ctgaaaaggc gaaatggcgt caccgcgagc gtcacgcgca
    10621 ttccgttctt gctgtaaagc gttgtttggt acacttttga ctagcgaggc ttggcgtgtc
    10681 agcgtatcta ttcaaaagtc gttaatggct gcggatcaag aaaaagttgg aatagaaaca
    10741 gaatacccgc gaaattcagg cccggttgcc atgtcctaca cgccgaaata aacgaccaaa
    10801 ttagtagaaa aataaaaact gactcggata cttacgtcac gtcttgcgca ctgatttgaa
    10861 aaatctcaga attcatggat cctaggtgag tctagagagt taattaagtt tgcaagcagc
    10921 agattacgcg cagaaaaaaa ggatctcaag gagatccttt ggatcgcaaa aaaaaagccc
    10981 gctcattagg cgggttaaat tcccgggtac tgcagccgat tcattaatgc aggttaacct
    11041 ggcttatcga aattaatacg actcactata gggagaccgg cagatctgat ccctactcca
    11101 aaaatgtcaa agatacagtc tcagaagacc aaagggctat tgagactttt caacaaaggg
    11161 taatttcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc atcgaaagga
    11221 cagtggaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggctatca
    11281 ttcaagatgc ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg
    11341 tgggaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt gacatctcca
    11401 ctgacgtaag ggatgacgca caatcccacc cctactccaa aaatgtcaaa gatactgtct
    11461 cagaagacca aagggctat
//
