LOCUS       sequence-172582-        8351 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral expression of CSF-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8351)
  AUTHORS   Roussel MF, Dull TJ, Rettenmier CW, Ralph P, Ullrich A, Sherr CJ
  TITLE     Transforming potential of the c-fms proto-oncogene (CSF-1 receptor).
  JOURNAL   Nature. 1987 Feb 5-11;325(6104):549-52.
  PUBMED    3027579
REFERENCE   2  (bases 1 to 8351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            1987 Feb 5-11"; volume: "325(6104)"; pages: "549-52"
COMMENT     Sequence Label: MSCV-(mcs) human CSF1 full length-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..8351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1697..2262
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1856..1873)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2083..2102
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2295..2894
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2295..2314)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2791..2811
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3169..3683
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    3845..3861
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3869..3875,3876..3893,3894..3899))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3914..3935
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4052..4069)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4223..4811)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4303..4322)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4982..5773,5774..5842))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5605..5624
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5843..5947)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6015..6033
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6071..6093)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6193..6212
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6406..6428
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6556..6575
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             6775..7291
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    7355..7696
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             7763..8179
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     8109..8131
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     8147..8163
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 atgaccgcgc cgggcgccgc cgggcgctgc cctcccacga catggctggg ctccctgctg
       61 ttgttggtct gtctcctggc gagcaggagt atcaccgagg aggtgtcgga gtactgtagc
      121 cacatgattg ggagtggaca cctgcagtct ctgcagcggc tgattgacag tcagatggag
      181 acctcgtgcc aaattacatt tgagtttgta gaccaggaac agttgaaaga tccagtgtgc
      241 taccttaaga aggcatttct cctggtacaa gacataatgg aggacaccat gcgcttcaga
      301 gataacaccc ccaatgccat cgccattgtg cagctgcagg aactctcttt gaggctgaag
      361 agctgcttca ccaaggatta tgaagagcat gacaaggcct gcgtccgaac tttctatgag
      421 acacctctcc agttgctgga gaaggtcaag aatgtcttta atgaaacaaa gaatctcctt
      481 gacaaggact ggaatatttt cagcaagaac tgcaacaaca gctttgctga atgctccagc
      541 caagatgtgg tgaccaagcc tgattgcaac tgcctgtacc ccaaagccat ccctagcagt
      601 gacccggcct ctgtctcccc tcatcagccc ctcgccccct ccatggcccc tgtggctggc
      661 ttgacctggg aggactctga gggaactgag ggcagctccc tcttgcctgg tgagcagccc
      721 ctgcacacag tggatccagg cagtgccaag cagcggccac ccaggagcac ctgccagagc
      781 tttgagccgc cagagacccc agttgtcaag gacagcacca tcggtggctc accacagcct
      841 cgcccctctg tcggggcctt caaccccggg atggaggata ttcttgactc tgcaatgggc
      901 actaattggg tcccagaaga agcctctgga gaggccagtg agattcccgt accccaaggg
      961 acagagcttt ccccctccag gccaggaggg ggcagcatgc agacagagcc cgccagaccc
     1021 agcaacttcc tctcagcatc ttctccactc cctgcatcag caaagggcca acagccggca
     1081 gatgtaactg gtacagcctt gcccagggtg ggccccgtga ggcccactgg ccaggactgg
     1141 aatcacaccc cccagaagac agaccatcca tctgccctgc tcagagaccc cccggagcca
     1201 ggctctccca ggatctcatc actgcgcccc cagggcctca gcaacccctc caccctctct
     1261 gctcagccac agctttccag aagccactcc tcgggcagcg tgctgcccct tggggagctg
     1321 gagggcagga ggagcaccag ggatcggagg agccccgcag agccagaagg aggaccagca
     1381 agtgaagggg cagccaggcc cctgccccgt tttaactccg ttcctttgac tgacacaggc
     1441 catgagaggc agtccgaggg atcctccagc ccgcagctcc aggagtctgt cttccacctg
     1501 ctggtgccca gtgtcatcct ggtcttgctg gccgtcggag gcctcttgtt ctacaggtgg
     1561 aggcggcgga gccatcaaga gcctcagaga gcggattctc ccttggagca accagagggc
     1621 agccccctga ctcaggatga cagacaggtg gaactgccag tgtagaggga attcgttaac
     1681 ctcgagcggg atcaattccg ccccccccct aacgttactg gccgaagccg cttggaataa
     1741 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     1801 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     1861 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     1921 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     1981 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     2041 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     2101 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     2161 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     2221 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aagcttgcca caacccacaa
     2281 ggagacgacc ttccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     2341 tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     2401 ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     2461 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     2521 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     2581 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     2641 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     2701 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     2761 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     2821 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     2881 agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac
     2941 cccatggctc cgaccgaagc cacccggggc ggccccgccg accccgcacc cgcccccgag
     3001 gcccaccgac tctagagctc gctgatccga cctgcaggca tgcaagcttc aggtagccgg
     3061 ctaacgttaa caaccggtac ctctagaact atagctagca tgcgcaaatt taaagcgctg
     3121 atatcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc
     3181 acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaatacat
     3241 aactgagaat agagaagttc agatcaaggt taggaacaga gagacagcag aatatgggcc
     3301 aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc
     3361 ccagatgcgg tcccgccctc agcagtttct agagaaccat cagatgtttc cagggtgccc
     3421 caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt
     3481 ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcggcg
     3541 cgccagtcct ccgatagact gcgtcgcccg ggtacccgtg tatccaataa accctcttgc
     3601 agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact
     3661 acccgtcagc gggggtcttt catgggtaac agtttcttga agttggagaa caacattctg
     3721 agggtaggag tcgaatatta agtaatcctg actcaattag ccactgtttt gaatccacat
     3781 actccaatac tcctgaaata gttcattatg gacagcgcag aaagagctgg ggagaattgt
     3841 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     3901 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     3961 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     4021 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     4081 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     4141 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     4201 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     4261 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     4321 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     4381 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     4441 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     4501 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     4561 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     4621 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     4681 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     4741 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     4801 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     4861 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     4921 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     4981 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     5041 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     5101 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     5161 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     5221 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     5281 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     5341 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     5401 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     5461 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     5521 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     5581 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     5641 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     5701 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     5761 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     5821 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     5881 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     5941 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     6001 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     6061 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     6121 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     6181 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     6241 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc
     6301 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     6361 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     6421 gtaaaacgac ggcgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc
     6481 cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc
     6541 ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc
     6601 ggtgatgccg gccacgatgc gtccggcgta gaggcgatta gtccaatttg ttaaagacag
     6661 gatatcagtg gtccaggctc tagttttgac tcaacaatat caccagctga agcctataga
     6721 gtacgagcca tagataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa
     6781 agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa
     6841 aatacataac tgagaataga gaagttcaga tcaaggttag gaacagagag acagcagaat
     6901 atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag
     6961 atggtcccca gatgcggtcc cgccctcagc agtttctaga gaaccatcag atgtttccag
     7021 ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt
     7081 ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca
     7141 ctcggcgcgc cagtcctccg atagactgcg tcgcccgggt acccgtattc ccaataaagc
     7201 ctcttgctgt ttgcatccga atcgtggact cgctgatcct tgggagggtc tcctcagatt
     7261 gattgactgc ccacctcggg ggtctttcat ttggaggttc caccgagatt tggagacccc
     7321 tgcccaggga ccaccgaccc ccccgccggg aggtaagctg gccagcggtc gtttcgtgtc
     7381 tgtctctgtc tttgtgcgtg tttgtgccgg catctaatgt ttgcgcctgc gtctgtacta
     7441 gttagctaac tagctctgta tctggcggac ccgtggtgga actgacgagt tctgaacacc
     7501 cggccgcaac cctgggagac gtcccaggga ctttgggggc cgtttttgtg gcccgacctg
     7561 aggaagggag tcgatgtgga atccgacccc gtcaggatat gtggttctgg taggagacga
     7621 gaacctaaaa cagttcccgc ctccgtctga atttttgctt tcggtttgga accgaagccg
     7681 cgcgtcttgt ctgctgcagc gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt
     7741 tctgtatttg tctgaaaatt agggccagac tgttaccact cccttaagtt tgaccttagg
     7801 tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca agaagagacg
     7861 ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc cgcgagacgg
     7921 cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac ctggcccgca
     7981 tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt ttgacccccc
     8041 tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc catccgcccc
     8101 gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt atccagccct
     8161 cactccttct ctaggcgccg gaattcgggg ggaaagtgaa agtttgcctg ggtcctctcg
     8221 gcgccagagc cgctctccgc atcccaggac agcggtgcgg ccctcggccg gggcgcccac
     8281 tccgcagcag ccagcgagcg agcgagcgag cgagggcggc cgacgcgccc ggccgggacc
     8341 cagctgcccg t
//
