LOCUS       sequence-172594-        9662 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses inactive/dead, humanized MbCpf1 nuclease.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9662)
  AUTHORS   Toth E, Czene BC, Kulcsar PI, Krausz SL, Talas A, Nyeste A, Varga E,
            Huszar K, Weinhardt N, Ligeti Z, Borsy AE, Fodor E, Welker E
  TITLE     Mb- and FnCpf1 nucleases are active in mammalian cells: activities 
            and PAM preferences of four wild-type Cpf1 nucleases and of their 
            altered PAM specificity variants.
  JOURNAL   Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 
            10.1093/nar/gky815.
  PUBMED    30239882
REFERENCE   2  (bases 1 to 9662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815."
COMMENT     Sequence Label: pTE4565
FEATURES             Location/Qualifiers
     source          1..9662
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        131..510
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        511..714
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     665..685
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     759..778
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        759..777
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4944..4991
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4998..5078
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(5152..5169)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5158..5382
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5428..5856
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5515..5534)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5725..5746
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5865..5885)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5870..6199
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6050..6185
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6112..6131
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6266..7060
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6320..6339)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6930..6949
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7234..7355
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7271..7290)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7325..7344
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7404..7420)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7404..7420)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7417..7439)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7428..7444
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7452..7458,7459..7476,7477..7482))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7497..7518
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7635..7652)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7806..8391)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7886..7905)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8562..9353,9354..9422))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9185..9204
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9423..9527)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9602..9621)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
       61 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
      121 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
      181 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
      241 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      301 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      361 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      421 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      481 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      541 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      601 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      661 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg
      721 ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag
      781 acccaagctg gctagcgttt aaacttaagc ttggtaccgc caccatgctg ttccaggact
      841 ttacccacct gtatccactg tccaagacag tgagatttga gctgaagccc atcgatagga
      901 ccctggagca catccacgcc aagaacttcc tgtctcagga cgagacaatg gccgatatgc
      961 accagaaggt gaaagtgatc ctggacgatt accaccgcga cttcatcgcc gatatgatgg
     1021 gcgaggtgaa gctgaccaag ctggccgagt tctatgacgt gtacctgaag tttcggaaga
     1081 acccaaagga cgatgagctg cagaagcagc tgaaggatct gcaggccgtg ctgagaaagg
     1141 agatcgtgaa gcccatcggc aatggcggca agtataaggc cggctacgac aggctgttcg
     1201 gcgccaagct gtttaaggac ggcaaggagc tgggcgatct ggccaagttc gtgatcgcac
     1261 aggagggaga gagctcccca aagctggccc acctggccca cttcgagaag ttttccacct
     1321 atttcacagg ctttcacgat aaccggaaga atatgtattc tgacgaggat aagcacaccg
     1381 ccatcgccta ccgcctgatc cacgagaacc tgccccggtt tatcgacaat ctgcagatcc
     1441 tgaccacaat caagcagaag cactctgccc tgtacgatca gatcatcaac gagctgaccg
     1501 ccagcggcct ggacgtgtct ctggccagcc acctggatgg ctatcacaag ctgctgacac
     1561 aggagggcat caccgcctac aatacactgc tgggaggaat ctccggagag gcaggctctc
     1621 ctaagatcca gggcatcaac gagctgatca attctcacca caaccagcac tgccacaaga
     1681 gcgagagaat cgccaagctg aggccactgc acaagcagat cctgtccgac ggcatgagcg
     1741 tgtccttcct gccctctaag tttgccgacg atagcgagat gtgccaggcc gtgaacgagt
     1801 tctatcgcca ctacgccgac gtgttcgcca aggtgcagag cctgttcgac ggctttgacg
     1861 atcaccagaa ggatggcatc tacgtggagc acaagaacct gaatgagctg tccaagcagg
     1921 ccttcggcga ctttgcactg ctgggacgcg tgctggacgg atactatgtg gatgtggtga
     1981 atccagagtt caacgagcgg tttgccaagg ccaagaccga caatgccaag gccaagctga
     2041 caaaggagaa ggataagttc atcaagggcg tgcactccct ggcctctctg gagcaggcca
     2101 tcgagcacta taccgcaagg cacgacgatg agagcgtgca ggcaggcaag ctgggacagt
     2161 acttcaagca cggcctggcc ggagtggaca accccatcca gaagatccac aacaatcaca
     2221 gcaccatcaa gggctttctg gagagggagc gccctgcagg agagagagcc ctgccaaaga
     2281 tcaagtccgg caagaatcct gagatgacac agctgaggca gctgaaggag ctgctggata
     2341 acgccctgaa tgtggcccac ttcgccaagc tgctgaccac aaagaccaca ctggacaatc
     2401 aggatggcaa cttctatggc gagtttggcg tgctgtacga cgagctggcc aagatcccca
     2461 ccctgtataa caaggtgaga gattacctga gccagaagcc tttctccacc gagaagtaca
     2521 agctgaactt tggcaatcca acactgctga atggctggga cctgaacaag gagaaggata
     2581 atttcggcgt gatcctgcag aaggacggct gctactatct ggccctgctg gacaaggccc
     2641 acaagaaggt gtttgataac gcccctaata caggcaagag catctatcag aagatgatct
     2701 ataagtacct ggaggtgagg aagcagttcc ccaaggtgtt cttttccaag gaggccatcg
     2761 ccatcaacta ccacccttct aaggagctgg tggagatcaa ggacaagggc cggcagagat
     2821 ccgacgatga gcgcctgaag ctgtatcggt ttatcctgga gtgtctgaag atccacccta
     2881 agtacgataa gaagttcgag ggcgccatcg gcgacatcca gctgtttaag aaggataaga
     2941 agggcagaga ggtgccaatc agcgagaagg acctgttcga taagatcaac ggcatctttt
     3001 ctagcaagcc taagctggag atggaggact tctttatcgg cgagttcaag aggtataacc
     3061 caagccagga cctggtggat cagtataata tctacaagaa gatcgactcc aacgataatc
     3121 gcaagaagga gaatttctac aacaatcacc ccaagtttaa gaaggatctg gtgcggtact
     3181 attacgagtc tatgtgcaag cacgaggagt gggaggagag cttcgagttt tccaagaagc
     3241 tgcaggacat cggctgttac gtggatgtga acgagctgtt taccgagatc gagacacgga
     3301 gactgaatta taagatctcc ttctgcaaca tcaatgccga ctacatcgat gagctggtgg
     3361 agcagggcca gctgtatctg ttccagatct acaacaagga cttttcccca aaggcccacg
     3421 gcaagcccaa tctgcacacc ctgtacttca aggccctgtt ttctgaggac aacctggccg
     3481 atcctatcta taagctgaat ggcgaggccc agatcttcta cagaaaggcc tccctggaca
     3541 tgaacgagac aacaatccac agggccggcg aggtgctgga gaacaagaat cccgataatc
     3601 ctaagaagag acagttcgtg tacgacatca tcaaggataa gaggtacaca caggacaagt
     3661 tcatgctgca cgtgccaatc accatgaact ttggcgtgca gggcatgaca atcaaggagt
     3721 tcaataagaa ggtgaaccag tctatccagc agtatgacga ggtgaacgtg atcggcatcg
     3781 cccggggcga gagacacctg ctgtacctga ccgtgatcaa tagcaagggc gagatcctgg
     3841 agcagtgttc cctgaacgac atcaccacag cctctgccaa tggcacacag atgaccacac
     3901 cttaccacaa gatcctggat aagagggaga tcgagcgcct gaacgcccgg gtgggatggg
     3961 gcgagatcga gacaatcaag gagctgaagt ctggctatct gagccacgtg gtgcaccaga
     4021 tcagccagct gatgctgaag tacaacgcca tcgtggtgct ggaggacctg aatttcggct
     4081 ttaagagggg ccgctttaag gtggagaagc agatctatca gaacttcgag aatgccctga
     4141 tcaagaagct gaaccacctg gtgctgaagg acaaggccga cgatgagatc ggctcttaca
     4201 agaatgccct gcagctgacc aacaatttca cagatctgaa gagcatcggc aagcagaccg
     4261 gcttcctgtt ttatgtgccc gcctggaaca cctctaagat cgaccctgag acaggctttg
     4321 tggatctgct gaagccaaga tacgagaaca tcgcccagag ccaggccttc tttggcaagt
     4381 tcgacaagat ctgctataat gccgacaagg attacttcga gtttcacatc gactacgcca
     4441 agtttaccga taaggccaag aatagccgcc agatctggac aatctgttcc cacggcgaca
     4501 agcggtacgt gtacgataag acagccaacc agaataaggg cgccgccaag ggcatcaacg
     4561 tgaatgatga gctgaagtcc ctgttcgccc gccaccacat caacgagaag cagcccaacc
     4621 tggtcatgga catctgccag aacaatgata aggagtttca caagtctctg atgtacctgc
     4681 tgaaaaccct gctggccctg cggtacagca acgcctcctc tgacgaggat ttcatcctgt
     4741 cccccgtggc aaacgacgag ggcgtgttct ttaatagcgc cctggccgac gatacacagc
     4801 ctcagaatgc cgatgccaac ggcgcctacc acatcgccct gaagggcctg tggctgctga
     4861 atgagctgaa gaactccgac gatctgaaca aggtgaagct ggccatcgac aatcagacct
     4921 ggctgaattt cgcccagaac aggaaaaggc cggcggccac gaaaaaggcc ggccaggcaa
     4981 aaaagaaaaa gggatcctac ccatacgatg ttccagatta cgcttatccc tacgacgtgc
     5041 ctgattatgc atacccatat gatgtccccg actatgccta agaattctgc agatatccag
     5101 cacagtggcg gccgctcgag tctagagggc ccgtttaaac ccgctgatca gcctcgactg
     5161 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     5221 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     5281 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     5341 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     5401 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     5461 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     5521 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     5581 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     5641 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     5701 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     5761 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     5821 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     5881 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     5941 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     6001 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     6061 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     6121 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     6181 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     6241 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     6301 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     6361 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     6421 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     6481 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     6541 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     6601 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     6661 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     6721 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     6781 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     6841 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     6901 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     6961 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     7021 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     7081 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     7141 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     7201 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     7261 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     7321 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     7381 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     7441 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     7501 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     7561 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     7621 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     7681 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     7741 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     7801 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     7861 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     7921 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     7981 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     8041 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     8101 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     8161 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     8221 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     8281 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     8341 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     8401 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     8461 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     8521 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     8581 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     8641 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     8701 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     8761 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     8821 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     8881 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     8941 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     9001 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     9061 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     9121 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     9181 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     9241 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     9301 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     9361 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     9421 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     9481 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     9541 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca
     9601 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg
     9661 ag
//
