LOCUS       sequence-17261-e         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Wei MC, Lindsten T, Mootha VK, Weiler S, Gross A, Ashiya M, Thompson
            CB, Korsmeyer SJ
  TITLE     tBID, a membrane-targeted death ligand, oligomerizes BAK to release 
            cytochrome c.
  JOURNAL   Genes Dev 2000 Aug 15;14(16):2060-71.
  PUBMED    10950869
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev";
            date: "2000-08-15"; volume: "14(16)"; pages: "2060-71"
COMMENT     Sequence Label: 3521 pET15b Bid p15
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             41..58
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..85
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(564..582)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      578..625
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tgtttaactt taagaaggag atataccatg gggcagcagc catcatcatc atcatcacag
       61 cagcggcctg gtgccgcgcg gcagccatat gggcagccag gccagccgct ccttcaacca
      121 aggaagaata gagccagatt ctgaaagtca ggaagaaatc atccacaaca ttgccagaca
      181 tctcgcccaa ataggcgatg agatggacca caacatccag cccacactgg tgagacagct
      241 agccgcacag ttcatgaatg gcagcctgtc ggaggaagac aaaaggaact gcctggccaa
      301 agcccttgat gaggtgaaga cagccttccc cagagacatg gagaacgaca aggccatgct
      361 gataatgaca atgctgttgg ccaaaaaagt ggccagtcac gcaccatctt tgctccgtga
      421 tgtcttccac acgactgtca actttattaa ccagaaccta ttctcctatg tgaggaactt
      481 ggttagaaac gagatggact gacatatgct cgaggatccg gctgctaaca aagcccgaaa
      541 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
      601 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atcccgcaag
      661 aagcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg
      721 aggatgacga tgagcgcatt gtt
//
