LOCUS       sequence-17262-e         670 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Wei MC, Lindsten T, Mootha VK, Weiler S, Gross A, Ashiya M, Thompson
            CB, Korsmeyer SJ
  TITLE     tBID, a membrane-targeted death ligand, oligomerizes BAK to release 
            cytochrome c.
  JOURNAL   Genes Dev 2000 Aug 15;14(16):2060-71.
  PUBMED    10950869
REFERENCE   2  (bases 1 to 670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev";
            date: "2000-08-15"; volume: "14(16)"; pages: "2060-71"
COMMENT     Sequence Label: 3535 pET15b Bid p22
FEATURES             Location/Qualifiers
     source          1..670
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..41
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             51..68
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gagatatacc atggggcagc agccatcatc atcatcatca cagcagcggc ctggtgccgc
       61 gcggcagcca tatggactct gaggtcagca acggttccgg cctgggggcc aagcacatca
      121 cagacctgct ggtgttcggc tttctccaaa gctctggctg tactcgccaa gagctggagg
      181 tgctgggtcg ggaactgcct gtgcaagctt actgggaggc agacctcgaa gacgagctgc
      241 agacagacgg cagccaggcc agccgctcct tcaaccaagg aagaatagag ccagattctg
      301 aaagtcagga agaaatcatc cacaacattg ccagacatct cgcccaaata ggcgatgaga
      361 tggaccacaa catccagccc acactggtga gacagctagc cgcacagttc atgaatggca
      421 gcctgtcgga ggaagacaaa aggaactgcc tggccaaagc ccttgatgag gtgaagacag
      481 ccttccccag agacatggag aacgacaagg ccatgctgat aatgacaatg ctgttggcca
      541 aaaaagtggc cagtcacgca ccatctttgc tccgtgatgt cttccacacg actgtcaact
      601 ttattaacca gaacctattc tcctatgtga ggaacttggt tagaaacgag atggactgag
      661 gatccggctg
//
