LOCUS       sequence-172620-       10020 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid clone containing HHV-6B(Z29) restriction endonuclease 
            fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10020)
  AUTHORS   Lindquester GJ, Inoue N, Allen RD, Castelli JW, Stamey FR, Dambaugh 
            TR, O'Brian JJ, Danovich RM, Frenkel N, Pellett PE
  TITLE     Restriction endonuclease mapping and molecular cloning of the human 
            herpesvirus 6 variant B strain Z29 genome.
  JOURNAL   Arch Virol. 1996;141(2):367-79.
  PUBMED    8634027
REFERENCE   2  (bases 1 to 10020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Arch 
            Virol."; date: "1996"; volume: "141(2)"; pages: "367-79"
COMMENT     Sequence Label: pH6Z-101
FEATURES             Location/Qualifiers
     source          1..10020
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3625..3641)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3625..3641)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3673..3692)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3674..3692)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3699..3716)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3699..3715)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3708..3730)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(3856..4311)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3993..4014)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4205..4224
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4338..4442
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4443..4511,4512..5303)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4661..4680)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5474..6062
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5963..5982
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6216..6233
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6350..6371
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6386..6391,6392..6409,6410..6416)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6424..6440
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6429..6451
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6448..6464
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6448..6464
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        6485..6503
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     6531..6547
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     6532..6548
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acatcaagaa ctaccagtaa cttgttgttt tgccatcttc tatattgtca tcagaaaagt
       61 catcagaaaa gtcatcagaa aaaaactaca ggatatctaa aatatttttc gaataccatt
      121 gaaaacatca aaaacatgtt caattcatca ccatatactt tgagtacaac tgcataacat
      181 tgttacaaat ttcaatatat gattttcgga ttgcgactcg gacaccggta acctcgctca
      241 cagtatttca tatattgtgt tgtaagatac ggacctcatg agtgtttgac gctcatgaag
      301 acggcaaagg cggctgcttt tgtccttttt actagttttc acacgttgta tcgaaatgaa
      361 gccagagatt attcacggct aatgatatga cccgatcaat acccggcgaa tggataaacg
      421 tgtctgaaac ctagaaaccg ctacgctctc ggaagaatta aaaaaccgcc aagagataga
      481 ccttgatcta tgtaataacg tgaagtgggc ctcgttgttt cataaatcaa agacgattgc
      541 tttagtagtg gggggttggg cacaaaacag ttgattattt agttcacatt ataaggacct
      601 aaaagttaag tatagttgat aagttaagtt tagtctataa gtgtagttat taagtaagtt
      661 aagcatctaa ctaatcattt tgtgattcca atcattactc ctggattttt gtttatctgg
      721 attcatacag aaacattgga tcatatgcag gcttttgtaa ataacctatt attactaaaa
      781 acaactctag gatttaatgg acggatctag catatttttt tcgtcatctt attcttaccc
      841 cgatacctag aataacttca gtagtgcaaa aaataaagga gcttcgattg atacttagga
      901 caccatccaa gccaccacct gagttaacat caaagtaact attaacgagt tcgcggctgc
      961 ttaataggct aaattgtgca tttttgaggt ttcccccgga gtacatacgt gtactccggg
     1021 ggagactttc atttgttttt gcagatgtca gctcatacca catccctgca gctgtattca
     1081 atttagcgac gaaacaaaat catggccgta agtcatttct catcagttcc tcatagtttc
     1141 acacccttgt ctttttcgag acatactgca ctaaatcccc gtgctacttt tgttttgcag
     1201 aaaaaacgga ccgacgggaa taagctcatt cgaccgtatg gaatttcaac aattacacgc
     1261 acaattcatc tgatgtttta gtttttttta tatattctca taaaaaacct ccctaaaata
     1321 ttaacatggt attttcgttc tttaacgctt ttacaatatg cggagcaata aaattatcat
     1381 tctcataatt cagtctcaag ggattaacat tcattattaa attattcggg tcggttactc
     1441 gtgacatccc aacataaata gaactcaatt ttaaattttt tggatcactc ccgaaatcta
     1501 ttgcgacctt ttgtatggaa agcccttgac ttttcgtgat ggtcatagaa actttagaaa
     1561 cagtggcgta atcgaccgtt gtacatagat gaaaactctg accatttacg acatcgacaa
     1621 atttcgacac gttattatct aaaacagaaa tgaaccccat ttcatctttt ataactaatc
     1681 taggtaatcc ttttttatat aagatctgcg gtaattgttt atccgttcct aaatgaacta
     1741 cattatcatt tgtataaccc tccagagtat aattattggc agggatagcg taggtgagca
     1801 taccataaaa actctttaaa tgggaatata tctcaccgca ctgttttcta gaaacgttgt
     1861 ttctgttata agtcactagg agagtctttc cgaacttccc ttttgtatgc ttctgcagaa
     1921 tggcaaacct agatataaag atatccttaa acgtcgtata aagcaaacta atttcctcga
     1981 acgacagaga attagttaaa ggcaacttac tatacctagt aaaaaatgga tctccgcaag
     2041 gatcaatttc taataggtcc atatctgata acccatcatc gactaatccg aaatcatacc
     2101 tttcatctgt ctctacggtt tgcacgtctc gagacatcct cgacatttca ctttctaaga
     2161 attcaatact gggcatcttt atgtttttga tctccattaa cacttcaggg gtggtgaatt
     2221 ccgaacagca gaaagaatac atccctccga acataagacc ggataaaaaa gaatatgcgt
     2281 gaatagtctg ctcacatgac gttttttcga taaacaaatc gctctgtaac agttctacaa
     2341 atttttcata agttccggaa aaccccacgg tcgaagcctt gatcttggac gtcacaccta
     2401 ttgaactgtt tctgatatgt ttcagatgac aggttatcaa agtctcttcc accgacccat
     2461 cttccaaaac aatctcctcc aattgaacag ttttcgataa atcctgatcc gcgaactgag
     2521 aataagtatt cagccgatga atattagatt taaaccacgg ttcaatttct aaggagaaat
     2581 taccaatttc acttttgtag tcttcgaaag tcttgttttt tattagaaaa taaaccggaa
     2641 acacaaacaa gttatttctg ttggtaactt caacctgctc atgcaatgag cggaaatagt
     2701 tctttacctc gttatgcgat aaaaacaagc gggtcgtttc tatttcgttc atgggatttc
     2761 ttatgtacga agcgggcttc acaaactgat cgacatactc tataagctcc ggctttaacg
     2821 gcaagccaaa ttctatgtgc tttaaaaagt ctccgaacgc atgatccgca catcttttgt
     2881 tgtttacaaa gattatccag ttattatcga cttcgcaata tttagagagc acgtcgtcgc
     2941 agatcaaggc tgaaagtaca tctattcctc tcttcacgtc cttgttttga gtcagcggat
     3001 taaatgaagt caccaaagcg ttactctgcg tgggcgaccc cacacacacg atgcacggaa
     3061 caattccgtt ctcatacaaa ggggttttat acaacgcgtt ataaaaccaa tagaaaaaaa
     3121 caacagtgtg cagaatatgt cgtaatatta cgcctgcctc gtcgataact ataacactac
     3181 tctcacacaa atcaggtaac tctttagttt tgcacgcgac agcacccaac gccctctccg
     3241 ctatgtcgga gataacattc caatagatag acaggtcttg cttttgttga atttgaatcg
     3301 acctctcgtc atttgcaata taactttgac gttcgctcat agatacatgt gaactattaa
     3361 acccaaaagt cttaaaaatt gttttaactt gcgcggattt acttctgttt aaaacaacgc
     3421 tcaaattctg agcggcgata gatgtcgttg cagtaacgat acaatctaag ttggccgcta
     3481 aggtctgaat gctgttcgtt ttccctgccc cggccgtacc ggttactaac aaggccttaa
     3541 acggcaaaaa aggcagaggt tgagcgaatt catcaatacc gatgcacaga gcatcagcaa
     3601 attcgccgtt gaactcggtt tcaggatcca ctagttctag agcggccgcc accgcggtgg
     3661 agctccaatt cgccctatag tgagtcgtat tacaattcac tggccgtcgt tttacaacgt
     3721 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     3781 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     3841 ctgaatggcg aatggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     3901 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     3961 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     4021 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     4081 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     4141 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     4201 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     4261 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca
     4321 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     4381 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     4441 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     4501 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     4561 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     4621 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     4681 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     4741 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     4801 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     4861 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     4921 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     4981 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     5041 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     5101 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     5161 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     5221 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     5281 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     5341 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     5401 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     5461 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     5521 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     5581 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     5641 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     5701 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     5761 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     5821 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     5881 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     5941 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     6001 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     6061 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     6121 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     6181 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     6241 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     6301 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     6361 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     6421 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     6481 tcgaaattaa ccctcactaa agggaacaaa agctggtacc gggccccccc tcgaggtcga
     6541 cggtatcgat aagcttgata tcgaattcct gcagcccggg ggatccgtat cccgacggca
     6601 gaggttgcgg tttggccttc ggtacagtga aagagtgctc gattccagaa tctctaaaga
     6661 atatatttaa agaactggaa agaagcatcc cgaatttttc cccccctgac aacggctgtt
     6721 taaactcctg gtgtagagaa ggagtcctac tgctaaactc gatattcact gtagttcatg
     6781 gattaccaat gtcccacgag gcatttggtt ggcaaacact gagctacaag attatcagca
     6841 gactatcgga acaaatgaac tctctcgttt tcttgttgtg gggaaaacat gcccggaaac
     6901 tctcttatct aatagacgca cagaaacatc tcgtcttaga aagtgcacat ccatcaccca
     6961 aagtgaaatc tgcaagaatg ccatttattg gttgcaatca ttttgtgcga acaaatttat
     7021 ttcttactga gcacgggaaa gacccaatca attggaacat tctgaacgaa tagaacctat
     7081 ttagtttaca taagaattac aatacagcaa taaaattcac agtgtttcca atattgtggt
     7141 tgccagtaag tcttcattaa aactcccagc attttcaaag ttttcggttt cgctgctatc
     7201 gtctatgaca attgcagaat tttgtgtcct taaattattt agattggaat tccgtctcct
     7261 gggaactctc cctcggccac gtcctcgtgc tctgccccgg ttttgtcctg aaataacacg
     7321 ctgctgcccg attgcaccat ctgttgatcc gacggttgtt ctggtccgtt gtcgaactct
     7381 tcttgcgttc tgcgttgttt cagtttcaaa tactcgcatg tcgataaacg gtttaatctc
     7441 acacacaggt accgacctga cacaatcaga aaacactatc tgtttctttt gctcgatatg
     7501 ttttgtttta cttaaacatt tacttggaac agtaacatca gcacattcaa gtacattttt
     7561 tattaaatta tcctttacat aatccaaacg ctctttcaat ttcgaaagcg atctttctac
     7621 aacctcgccg ctgcttgcaa caagtctggc cctaagctgt tctaaagact tattaagctg
     7681 aaaatttcca taattatctt cattggatag accttctcgg ttcgtcccct aaaagaaaaa
     7741 gatcagcttt agatacgcac aacaaagact ccaaagacct aggttttggt gaacccaaaa
     7801 ctccacccac ctgcgcataa acgcgcttgt ctaattcatc atgttgttga tcttcgtgat
     7861 tatcaataga aacacagggc gataacgcac cctcaaccgc tccattcacg aatgtcgtag
     7921 aagtcggtgc tatgccatct tcgtgacttt gttcttccaa attacgtttc gtttcgtggt
     7981 gagatttctg tctcttctcc ttagggggtt ctgaaatcac aacaaaacag atactattta
     8041 aaaaagtcca cgaccgtgac acaccaataa taaaaaaatt tacaacagct acacattcat
     8101 atttaccatc aaattcatcc tttttgtccg gccgcttgtc ttcgtcgttt cgtttttttt
     8161 cttcttgttt tcgtttctca tcttcttttt ttttgtcaat gtcctccact tttctttttt
     8221 ctttctgctt ccccgagcgt tttctgtgtt catcgtcgtg ttttttcctg tctagagctc
     8281 gacattcttt ttcaaacttt tccataaatt tcaaatcgcc cagatttcgt agctcgggct
     8341 ccgaggaaac tttagcaata gtaacaaacg ttatttgaag ttttccgttt tcatgggaaa
     8401 cagtcaaaat cctctctctg ccctcagtat tctgagaaat cgacgtgatt gattttgaac
     8461 acagtaaaag gccccgagct ctgtacgttc tgcattttcc ttcatccatc ccaatatcca
     8521 ccgttagaga acccattgaa tctgtctccg atgtccaata aatcattgat atccatcctt
     8581 tagaaaacgt ttcacacgtt ccagagtcag ctaagaactc aatgaaacag ccattgtcga
     8641 attcaaacct tttcaaatga agagactgac taataatttg ttcataagcc tgtataacat
     8701 tgtcaaatga cccatatcca cgttcttcag catacatctt atctgtgtga aaataagaac
     8761 aaaataaaca tgctcgacac catcaaaata cacatagcaa atcgtaacat tacagaaatc
     8821 caaaaacgac tacaaacgag acatacattt ttaccagaat aatcccagaa aaatctcaga
     8881 aagatgagtt ctttgccgct catctagcac aaatgagcta gcgaacacag tcaccagcgc
     8941 ccttttatgc gcacgaatca cacttccggg ctcgagcgtt tgcgatccaa aaactactgc
     9001 atacaactaa aacgatttat ttctcccatg aaacgacata tctaagtcag acaacgaaca
     9061 cgatataaaa atctattaaa ccgaaatgtg gcccgacatc taaaaatacc gttcggcttt
     9121 tttactgtaa cgacttttta ctaacggtta accacatcct catccaaacc gaaaaccttt
     9181 acgtattgcg tcagcgacaa gggcgtggac tttgccgtaa aatgcatata aaaaggcttg
     9241 tcgcccgtca gaagtattag ggatcaaact gtaagtctca tacacggcaa aacagcgaat
     9301 gctaaaccgc gaactacaaa tcacgtgaag cttgatgcag actcttatac agattgaatg
     9361 tatacacttt gatacattat gaagcataaa tactaaaatg tagttagatc gaaatgaaag
     9421 ctttatggga ctattaaaat tgcagattca tgcttatgta aacgtgaata aattcggtgt
     9481 ttttgatgtt ccgtgaaaaa gtttacagcg cattcgacat atggaattta cgtctactcg
     9541 aacgttgacc tgttgtagct tcaaggtaag atttaaccta ttttgcatct acgcacttcc
     9601 aagagctcaa tacaattttg tatatgccaa aaaacccctt tagtcttttt aatagaaaat
     9661 cgtttatcaa ccaagatgta agttagttta gtttccagcg tatagttatc gagggacaca
     9721 tcattaaaat ctgcgttgca taaaaacttg gactgctaaa aattacactt gcttaaagaa
     9781 atagtcatct agttttcgga aaaaaccctc gttcacacca tgctagcctg aatggaaaat
     9841 cgtaggtcac tggttctttc tatacgagat gagtatgctt gcttattttt ctctattgtc
     9901 gtcaattgct tctggctgtg gttctcatag aatttccccc gtctgtgtgg aaacaccctt
     9961 caattttact cgtatcacct acctctctct acttgtcata tctccgattc attttatcta
//
