LOCUS       sequence-172622-        6758 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid clone containing HHV-6B(Z29) restriction endonuclease 
            fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6758)
  AUTHORS   Lindquester GJ, Inoue N, Allen RD, Castelli JW, Stamey FR, Dambaugh 
            TR, O'Brian JJ, Danovich RM, Frenkel N, Pellett PE
  TITLE     Restriction endonuclease mapping and molecular cloning of the human 
            herpesvirus 6 variant B strain Z29 genome.
  JOURNAL   Arch Virol. 1996;141(2):367-79.
  PUBMED    8634027
REFERENCE   2  (bases 1 to 6758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Arch 
            Virol."; date: "1996"; volume: "141(2)"; pages: "367-79"
COMMENT     Sequence Label: pH6Z-109
FEATURES             Location/Qualifiers
     source          1..6758
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1883..1899)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1884..1900)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(1928..1946)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1967..1983)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1967..1983)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1980..2002)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1991..2007
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2015..2021,2022..2039,2040..2045))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2060..2081
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2198..2215)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2369..2957)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2449..2468)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3128..3919,3920..3988))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3751..3770
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3989..4093)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4120..4575
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4207..4226)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4417..4438
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4701..4723
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4715..4732
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4716..4732
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4739..4758
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4739..4757
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     4790..4806
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4790..4806
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gtctatgcgc gcgccgtgac acggaaaacc aagcggagcg agacgatcga ccgactcctg
       61 ctatccgtcc tccctgggca cggtccacac gccagtctgc ggagtcacct gagggccaga
      121 tccgctctgc gccccccgcc cgatccgccc cgataaaaaa aaatcatctg actcgtgcca
      181 gttcacacag atgcaaccga tgacaaaaaa aaaacacacc accgcaagca aacgctcgcc
      241 ggcccgtcca ccgtccctct cacccccact cgaatcgcgc aggcgcgtcg gcgggaagtc
      301 ccccgaacgc ctccacggca acgctcctcc cacgccccct cggtcccccc tccctccatc
      361 cccctccccg gagcatacgc gccgactctc tccgcagagg cggcgaccac agcggcagcg
      421 gaaactgtaa cggccacgtt ctttgaacag tgacagtaac ggcggcggcg gctgtcgccg
      481 tcacggtagt cgtggcggag cacccgggga ggcgacgacg gggactaccg atgctctcag
      541 atcatcgcca tagtgaaaga gtaccgaata aaacaagtta aatttcgttg taaataaaaa
      601 aaaatactat gtggaaatta aaaataaaaa aaatgaaaat aaaggctaaa cactgactaa
      661 acgtacatcc ttctccgtac gtcccctttc aataaatggc ggacccgacc tctccataaa
      721 cggagacacg ccgggagact ccggccctcc cgttccagag gtagcccgag acctaatcac
      781 aaaactgtac ctgaaaccga aagtatagag ggcggcctcg aatgccccac ctatgaggcg
      841 ggacttcgcg acgcgccacc cttcctatac gcccggcctt ccgcgtgcgc gcctatagga
      901 cgcatcccct cgccccgaaa ccgcaacgcg gcagagtcgg cgccacaccc ccgtcccccg
      961 agttcgcgat gggctggcgc cgaccctgcc gcggcaacga aggcgaaacg agaccccgac
     1021 gcatgcgggc ataaagagcg agaagtaaac gcggggtccc gaattatcgc gagactattt
     1081 gcccgggccc gccctcgtcc tcatgccctt ccccccgccc tcatcccctg acacacccgg
     1141 tggaaaaaac gaaagacaca taaataaaac gaattttgtt gttaaaaatg tttccacgtg
     1201 acgtccccgg tgattcccgg agatgcggca cgcggcggat tcgcccgttt acatcggggt
     1261 ccgcagacgc ggttctgcca gggcgttgcg ctgtcggttc cgcacaaaac accgcggtcc
     1321 gtcgtcaccc ttcagccacg cggtagttcc gcgattctat ggttcatctc cgctcacatc
     1381 gttctttttt ttttattctt tcactgagcc gctccaccac caagagtgtt actatctaca
     1441 ccgtatacat gttttttttt tatctctccg cccctcccca tcacagacca tcaaacacct
     1501 tttgccgtat cgacactgac tatactcgac cgcggatgac aacggtcgtt ggtcacaaca
     1561 gagctacagc ggacagcaaa aataacataa atacagtaag agagaggccc cggtccacat
     1621 aggaagccaa gggcggacga aaataaaaac ccgtccgtaa ttcacacacg aatcgggggt
     1681 tatgacaacg cgacacacgc agacgaggga cggacgcatc gcgatccggc gcgacggggc
     1741 gcgattggcc cacgcgcgag cccgtgcccg cttcgagtgg ctgctcctgg ctcgcggcag
     1801 gccgtccaaa ctgtacggct atacgagtcg gcaccgggga gaacggatcc cccgggctgc
     1861 aggaattcga tatcaagctt atcgataccg tcgacctcga gggggggccc ggtaccagct
     1921 tttgttccct ttagtgaggg ttaatttcga gcttggcgta atcatggtca tagctgtttc
     1981 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     2041 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     2101 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     2161 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     2221 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     2281 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     2341 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     2401 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     2461 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2521 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     2581 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     2641 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2701 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2761 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     2821 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2881 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2941 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     3001 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     3061 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     3121 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     3181 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     3241 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     3301 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     3361 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     3421 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     3481 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     3541 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     3601 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     3661 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     3721 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     3781 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     3841 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     3901 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     3961 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     4021 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     4081 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat
     4141 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     4201 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     4261 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     4321 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     4381 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     4441 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     4501 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     4561 taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     4621 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     4681 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattgta
     4741 atacgactca ctatagggcg aattggagct ccaccgcggt ggcggccgct ctagaactag
     4801 tggatcccgc tcggagctct ctcaccccgt gttggagtgg gagagaacag agctggtcct
     4861 gacggatcgg agacgcgggt ggccgtgtac gcacctcctg tccggctcgg agtcccagcg
     4921 cgtttcctcg agtgacgccg gagacacatg gaacgcagcg acagagaagg cggcgggggg
     4981 aaaagaggag gcggagagag gcgggcgaca gcaagccact gacagactcg caagtccgca
     5041 cctgacgcgg ggccgccgcg actgcggtcg gtcccttcag ggtgaggagc ccagcgccgc
     5101 ggaagacttt gcgaggtgca gaccgctgct ggacgaactg tgcggggagg gcggctggct
     5161 tccctttgcg tttctcacgg catctccgca cgtctgtctg atcctaacgg agggaggccc
     5221 cgtcctggcg cttgacctga acgacacctc cctgtggcgc atcgcggacg acttggagct
     5281 gctgctgcgc ctggggagcc tgctcctgct ctcagggctc cggcttcctc tccgtccccc
     5341 gagcgggagc ggcgaggcgg cgagaaagcc ggggtacgag aaggaagagg gaagagggag
     5401 agcgacgacg gcgagcgcga cggccgcgac gtcgccgcgc agaccgaccc gtccgagggg
     5461 ggtgacggag aagggacgtg tgacaaccgg ggacgtccct ttctccgcac atcccgaatc
     5521 tgaggaacag acagacggcc accacgggcg ccaagaaagc ggccacggcg accagcgcgg
     5581 cggggacgga cgaggacacc gcgatgacgg cgcgcgccgc cacgcgaatg acgaaacaga
     5641 gccccagcag cgcggagagc acgaggacgg ggaacagacc gactccgggc gcgaggagga
     5701 cgcacaggag agcgaggtcg caagaagaga cgagaaggga acggagcagg gcggtagcgg
     5761 aaggagctgc gggagggcaa cgcagacgta cggcgggaga ggcgaacatg gtgcctggtc
     5821 gtcgatcccc ctgtctgtcc ccaggcccga tccccgcgtg tgggtccctc ctcctcatct
     5881 gttattccct tccccgctgc cgtcgataac gcccgtcgaa gacgagccgt ccgcccgccc
     5941 tcggtgcccg ccaggtcccg cggaggaacc ctctaagtgt tctccgtgcc cgccctgccc
     6001 gtcgcccgac gccccgcagt ccgctgtccc gcgcctctcc gctctgtccg tcccctcgcc
     6061 gtccaccgcg cgtgtccgtt tctccctctc ctccctctcc tcctcctcct cctcctcttc
     6121 ctcctcctcc ccgtcctact ctccctcccc gttgtctccg ccctctcccg tctctccctc
     6181 gtctcccaga tctccgttca tctcccccat tagatctccg ggactccgag caaagccgtg
     6241 ggtgtcctcc gggcatcccg tggcgttccc gccggcgccc tcgtccgcgc cgcccttttc
     6301 caaaagagtc ccgtccgttc cctcatcggc atctccgtcc gcgccgtgca tcggcaggtc
     6361 gcggccgccc tccgcacaga cggcgtgaga cgccgcgtga ggtgacggag gcggcgaagg
     6421 gacacgagaa gaacatgcag aagaacatga agacgaagaa gacgaagaaa cgaggacgaa
     6481 aggaggggaa tacaccggag accgagcggc ggatggagcc cgctcggtct cgcacgtccg
     6541 cgataccgag cgggctccgt cgccgctccg gaccctccac gcccctccgt ccaggacccg
     6601 aggtccgaca cgcccccacc tggcgcaccg cctccgccac caccgctgac agtcaccgca
     6661 tatcaccccc atacacaccc agcagcagag gccggcacat acacacgcgc ggagcacgca
     6721 cgagaacacg cgagacatcg gccgccgaga ttaacgga
//
