LOCUS       sequence-172623-        5153 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid clone containing HHV-6B(Z29) restriction endonuclease 
            fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Lindquester GJ, Inoue N, Allen RD, Castelli JW, Stamey FR, Dambaugh 
            TR, O'Brian JJ, Danovich RM, Frenkel N, Pellett PE
  TITLE     Restriction endonuclease mapping and molecular cloning of the human 
            herpesvirus 6 variant B strain Z29 genome.
  JOURNAL   Arch Virol. 1996;141(2):367-79.
  PUBMED    8634027
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Arch 
            Virol."; date: "1996"; volume: "141(2)"; pages: "367-79"
COMMENT     Sequence Label: pH6Z-203
FEATURES             Location/Qualifiers
     source          1..5153
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(730..746)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(730..746)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(778..797)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(779..797)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(804..821)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(804..820)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(813..835)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(961..1416)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1098..1119)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1310..1329
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        1443..1547
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1548..1616,1617..2408)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1766..1785)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2579..3167
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3068..3087
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3321..3338
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3455..3476
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3491..3496,3497..3514,3515..3521)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3529..3545
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3534..3556
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3553..3569
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3553..3569
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        3590..3608
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     3636..3652
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3637..3653
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aacgtaagtt ggagtttata ctatctgcga tgtatcgttt gaaagagttt atatttgaga
       61 tttcttaact tgtgtttctc acagagcgtt taaaaatgca ctgtacgaaa ctgaggaact
      121 gatggaaacc atcggaatgc gacttgcctc acaaatattg tcactggtcg gacagcgagg
      181 aacatctata cggtgagttt aaataatagg ggattggtag gagcgaattt tttttttagt
      241 aaccagttat attaatttat ttttttcgct tttttcggtt atgcacagca acatagatcc
      301 ggccatcgta agcgcattgt ggcactcttt gccagaaaaa ctgacaacga ctaatataaa
      361 atacgatata gccagtccga cacatatgag tcccgcatta tggtgagcgc tgttaaacta
      421 tgaatctaaa tgtagagttt ttttttacgt ttaatttcac tttctttgga ttagaagacg
      481 aaaggccata attaaacaaa tgacttttcc agagtttttt tttatattta tgcagttttt
      541 cttaatccgc agcaccatct ttatacagac cggaacttca aaacaacgtt tcagaaatgc
      601 gggactactg atggttaaca acatcttcac tgtgcaagcg agatatagta aacaaaatat
      661 gttcgaaaaa aaaatatatg gatatgaaca cctcggacaa gcttgatatc gaattcctgc
      721 agcccggggg atccactagt tctagagcgg ccgccaccgc ggtggagctc caattcgccc
      781 tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
      841 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
      901 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
      961 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     1021 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     1081 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     1141 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     1201 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     1261 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     1321 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     1381 cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg
     1441 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     1501 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     1561 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     1621 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     1681 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     1741 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     1801 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     1861 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     1921 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     1981 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     2041 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     2101 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     2161 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     2221 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     2281 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     2341 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     2401 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     2461 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     2521 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     2581 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     2641 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     2701 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     2761 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     2821 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     2881 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     2941 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     3001 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     3061 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     3121 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     3181 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     3241 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     3301 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     3361 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     3421 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     3481 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     3541 aacaatttca cacaggaaac agctatgacc atgattacgc caagctcgaa attaaccctc
     3601 actaaaggga acaaaagctg gtaccgggcc ccccctcgag gtcgacggta tcgataagct
     3661 tttgtccccg tagattatac cccaactact accttagaag atatagaaga cctcctgaac
     3721 tacgccattt gcgatgataa cagctgcggt tgtctcatag agacagaggc acggtaagga
     3781 tctagcgttt tattttattt catcttgttt ctttaccacg cgctcattct cccggacacc
     3841 gatgtcggaa tccgccgaga taacaaataa atgaatatat aataatcata tctaattccc
     3901 cgcagcatca tgttcggaga tatcataatc tgcgttcccc tctcggccga aagcagagga
     3961 gtgagaaatt taaaaagcag aataatgcct atgggtctat cacagattct ctctagcggc
     4021 ctcggccttc acttctctct gctatatggc gctttcggga gcaactataa tagtttggcc
     4081 tatatggaac gcctgaaacc actaaccgcg atgacggcga tagctttttg tccaatgact
     4141 agtaaattgg agctacgcca gaactacaga ctggaaaagg ctcgatggtg agtacttgta
     4201 caagtttttg tgccttcccg ccacgtctcc tttatcgaca aggaaaggca tgcattgtgt
     4261 atttattcac atagaaaagc gtaagatccc cgccgtataa atatcccgaa ttcgctaaaa
     4321 agagtcccat ttatacaccc cgccccgccc tcggatgcac ctgtcactct aaaaatgtcg
     4381 cagcacttct cctccaccct ccactccacc ccctcttaga agacagacca cacccatgta
     4441 cgcgcatacg tgtcaacccc acacgcatac ctacatcctt tcagagcaga aactatgggg
     4501 ctggaagcct tcgtaacaag aaaatttttt ctcgttcctt gtatcttgcc tctttgtgcg
     4561 gcacacatgt attcatccac tcaaaatcca gcgaactgat cgtcaatatc gaactcttga
     4621 agatacaaaa ccatggcgga cagactatca aaacgttaac ctcgtttgcg atcgttcgaa
     4681 aagacagcga cgggcaagat tgggaaacct gcacgcgctt cgcgtccgtt agcatagagg
     4741 acatattgag gtctaaacct gccgccaatg gcacatgttg tcctccccga gacgtgcacc
     4801 atgatcgtcc tacgttacaa tcttctaact catggactcg gactgagtat ttcgaaccgt
     4861 ggcaggacgt cgttgacgca tacgtcccta taaacgataa tcattgtcca aacgatagct
     4921 acgtcgtgtg agtgattcat gccaatttta ataaaaaact ccatctatgt tttttattgg
     4981 ttttgctaca tagcagtgga acacaattct gaaattctaa tttcgaaatt cgcttttttt
     5041 ttcgtcagat ttcaaaccct gcaaggtcac gaatggtgtt cacgtttgaa taagaacgac
     5101 acgaagaatt atttatcttc tgtactgtaa gtaaacttat tttctcttct ttg
//
