LOCUS       sequence-172625-        8328 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid clone containing HHV-6B(Z29) restriction endonuclease 
            fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8328)
  AUTHORS   Lindquester GJ, Inoue N, Allen RD, Castelli JW, Stamey FR, Dambaugh 
            TR, O'Brian JJ, Danovich RM, Frenkel N, Pellett PE
  TITLE     Restriction endonuclease mapping and molecular cloning of the human 
            herpesvirus 6 variant B strain Z29 genome.
  JOURNAL   Arch Virol. 1996;141(2):367-79.
  PUBMED    8634027
REFERENCE   2  (bases 1 to 8328)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Arch 
            Virol."; date: "1996"; volume: "141(2)"; pages: "367-79"
COMMENT     Sequence Label: pH6Z-201
FEATURES             Location/Qualifiers
     source          1..8328
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(48..67)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(49..67)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(74..91)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(74..90)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(83..105)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(231..686)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(368..389)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     580..599
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        713..817
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(818..886,887..1678)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1036..1055)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1849..2437
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2338..2357
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2591..2608
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2725..2746
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2761..2766,2767..2784,2785..2791)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2799..2815
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2804..2826
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2823..2839
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2823..2839
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2860..2878
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2906..2922
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2907..2923
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     mobile_element  6773..7540
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     primer_bind     complement(join(8328,1..16))
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(join(8328,1..16))
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 atccactagt tctagagcgg ccgccaccgc ggtggagctc caattcgccc tatagtgagt
       61 cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
      121 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
      181 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg aaattgtaag
      241 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
      301 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
      361 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
      421 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
      481 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
      541 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
      601 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
      661 gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac
      721 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
      781 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
      841 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
      901 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
      961 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     1021 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     1081 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     1141 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     1201 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     1261 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     1321 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     1381 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     1441 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     1501 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     1561 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     1621 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     1681 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     1741 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     1801 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1861 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     1921 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     1981 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     2041 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     2101 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     2161 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     2221 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     2281 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     2341 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     2401 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     2461 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     2521 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     2581 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     2641 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     2701 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     2761 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     2821 cacaggaaac agctatgacc atgattacgc caagctcgaa attaaccctc actaaaggga
     2881 acaaaagctg gtaccgggcc ccccctcgag gtcgacggta tcgataagct tgtcggcatt
     2941 tgctctctca tgataaacgc gccgacggaa aaagatttat tatgcgccgc tcacgtttgt
     3001 atcaattttg caatctgcca tcccaacgag attactctga atatcgcgaa gctaacacaa
     3061 ccgatgctcc ttcgagaaca catcaccata tacaactctt tcaacaaata tatttattgg
     3121 gatgattatg gcaaactatt aaactgacaa aataaacttt tttggctcga atctaaattg
     3181 gtctctcttt gcgtcctcta tattgatcgt cataacgaga aagaaaacca gtattaatgt
     3241 cttcgaatag tttcatgatt ctttgtctaa tttcgacaat cctaattcaa gacttgtcgg
     3301 cgatgcgttt gccatatcac acatcgagcc ctgctctaaa tcagacaggt aagcagcaaa
     3361 acaacttttt agctttttaa tcatgattgc aacaaaaggt tttctgaaag tctctgtctt
     3421 cccaggtacg acagcggacc aatctgtgca acacaacaaa catcctaata agacagaaga
     3481 cgaaatacgt gaattttctg aagacagctc cgcatcgggg gaactaccga taaatccgat
     3541 agccatacca atatatgccg cataaaaaaa aacaataaaa agccaaatga acatttcgac
     3601 aagtcttttt ccctactgta tataagatgt tgttcccgtc gtgttaactt ctccaaataa
     3661 aaaataacca tggaactttt actatttgta atgtcactca tattactaac cttctcgaaa
     3721 gcgatgcctc ttttcgacca caattctttc tattttgaaa aacttgacga ctgtatcgca
     3781 gctgtaataa attgcacgag atccgaagta cctctattac tagaaccaat ctaccagcct
     3841 ccggtatata atgaagacgt tatgtcaata ctgctaaaac ccccaacaaa aaaaaagcct
     3901 tttagccgta taatggtaac caatgaattc ctcagcgact tcttacttct gcaggataac
     3961 ccagagcaac tacgcacatt gtttgcgctg ataggggacc cagaatctcg ggacaattgg
     4021 ctaaactttt tcaatggctt ccagacatgt tcgccttccg tcggaataac aacctgcatc
     4081 agcgataact gtagaaaata tttgcctgaa agaattacgt acgtcaataa cttttttgtt
     4141 gataacattg caggtctcga gtttaacatt tcagaaaaca cagacagttt ttacagcaac
     4201 attggttttt tattatactt ggagaatcct gctacaggca tcacaaaaat tatcaggttc
     4261 ccttttaact ctttgactct ctttgatacg attttgaatt gtttaaagta tttccacttg
     4321 aaaaccggag tagaattcga cctgctaaaa cagatggaag cctacaattc taaactacct
     4381 ttccgaagtt cccgccctac gattctgatt agaaacacat aattggctat gacatcacgt
     4441 cccattcaca taaaatgctc ctagggaatt taatccactg catttcattt cccaaccatg
     4501 gccctactac agtggatgtt agatcacgtt caggatgaag aaaaaaacta tgaaaacctt
     4561 tcaatagatg atcagcattc actcttcggc ataaacagag actggttgag tttcctgcaa
     4621 ctctccaagt tagaaattac acatcttaag catgtttata aattggtgga caacgataga
     4681 gttcatctaa ccgtccaccc ctcttcggat aacgtccacg cctggagttt tttgtgcaaa
     4741 cccaccgatg ttaaagttgt gattctggga caggatccgt atcccgacgg cagaggttgc
     4801 ggtttggcct tcggtacagt gaaagagtgc tcgattccag aatctctaaa gaatatattt
     4861 aaagaactgg aaagaagcat cccgaatttt tccccccctg acaacggctg tttaaactcc
     4921 tggtgtagag aaggagtcct actgctaaac tcgatattca ctgtagttca tggattacca
     4981 atgtcccacg aggcatttgg ttggcaaaca ctgagctaca agattatcag cagactatcg
     5041 gaacaaatga actctctcgt tttcttgttg tggggaaaac atgcccggaa actctcttat
     5101 ctaatagacg cacagaaaca tctcgtctta gaaagtgcac atccatcacc caaagtgaaa
     5161 tctgcaagaa tgccatttat tggttgcaat cattttgtgc gaacaaattt atttcttact
     5221 gagcacggga aagacccaat caattggaac attctgaacg aatagaacct atttagttta
     5281 cataagaatt acaatacagc aataaaattc acagtgtttc caatattgtg gttgccagta
     5341 agtcttcatt aaaactccca gcattttcaa agttttcggt ttcgctgcta tcgtctatga
     5401 caattgcaga attttgtgtc cttaaattat ttagattgga attccgtctc ctgggaactc
     5461 tccctcggcc acgtcctcgt gctctgcccc ggttttgtcc tgaaataaca cgctgctgcc
     5521 cgattgcacc atctgttgat ccgacggttg ttctggtccg ttgtcgaact cttcttgcgt
     5581 tctgcgttgt ttcagtttca aatactcgca tgtcgataaa cggtttaatc tcacacacag
     5641 gtaccgacct gacacaatca gaaaacacta tctgtttctt ttgctcgata tgttttgttt
     5701 tacttaaaca tttacttgga acagtaacat cagcacattc aagtacattt tttattaaat
     5761 tatcctttac ataatccaaa cgctctttca atttcgaaag cgatctttct acaacctcgc
     5821 cgctgcttgc aacaagtctg gccctaagct gttctaaaga cttattaagc tgaaaatttc
     5881 cataattatc ttcattggat agaccttctc ggttcgtccc ctaaaagaaa aagatcagct
     5941 ttagatacgc acaacaaaga ctccaaagac ctaggttttg gtgaacccaa aactccaccc
     6001 acctgcgcat aaacgcgctt gtctaattca tcatgttgtt gatcttcgtg attatcaata
     6061 gaaacacagg gcgataacgc accctcaacc gctccattca cgaatgtcgt agaagtcggt
     6121 gctatgccat cttcgtgact ttgttcttcc aaattacgtt tcgtttcgtg gtgagatttc
     6181 tgtctcttct ccttaggggg ttctgaaatc acaacaaaac agatactatt taaaaaagtc
     6241 cacgaccgtg acacaccaat aataaaaaaa tttacaacag ctacacattc atatttacca
     6301 tcaaattcat cctttttgtc cggccgcttg tcttcgtcgt ttcgtttttt ttcttcttgt
     6361 tttcgtttct catcttcttt ttttttgtca atgtcctcca cttttctttt ttctttctgc
     6421 ttccccgagc gttttctgtg ttcatcgtcg tgttttttcc tgtctagagc tcgacattct
     6481 ttttcaaact tttccataaa tttcaaatcg cccagatttc gtagctcggg ctccgaggaa
     6541 actttagcaa tagtaacaaa cgttatttga agttttccgt tttcatggga aacagtcaaa
     6601 atcctctctc tgccctcagt attctgagaa atcgacgtga ttgattttga acacagtaaa
     6661 aggccccgag ctctgtacgt tctgcatttt ccttcatcca tcccaatatc caccgttaga
     6721 gaacccattg aatctgtctc cgatgtccaa taaatcattg atatccatcc ttggtgatgc
     6781 tgccaactta ctgatttagt gtatgatggt gtttttgagg tgctccagtg gcttctgttt
     6841 ctatcagctg tccctcctgt tcagctactg acggggtggt gcgtaacggc aaaagcaccg
     6901 ccggacatca gcgctatctc tgctctcact gccgtaaaac atggcaactg cagttcactt
     6961 acaccgcttc tcaacccggt acgcaccaga aaatcattga tatggccatg aatggcgttg
     7021 gatgccgggc aacagcccgc attatgggcg ttggcctcaa cacgatttta cgtcacttaa
     7081 aaaactcagg ccgcagtcgg taacctcgcg catacagccg ggcagtgacg tcatcgtctg
     7141 cgcggaaatg gacgaacagt ggggctatgt cggggctaaa tcgcgccagc gctggctgtt
     7201 ttacgcgtat gacagtctcc ggaagacggt tgttgcgcac gtattcggtg aacgcactat
     7261 ggcgacgctg gggcgtctta tgagcctgct gtcacccttt gacgtggtga tatggatgac
     7321 ggatggctgg ccgctgtatg aatcccgcct gaagggaaag ctgcacgtaa tcagcaagcg
     7381 atatacgcag cgaattgagc ggcataacct gaatctgagg cagcacctgg cacggctggg
     7441 acggaagtcg ctgtcgttct caaaatcggt ggagctgcat gacaaagtca tcgggcatta
     7501 tctgaacata aaacactatc aataagttgg agtcattacc tccatccttt agaaaacgtt
     7561 tcacacgttc cagagtcagc taagaactca atgaaacagc cattgtcgaa ttcaaacctt
     7621 ttcaaatgaa gagactgact aataatttgt tcataagcct gtataacatt gtcaaatgac
     7681 ccatatccac gttcttcagc atacatctta tctgtgtgaa aataagaaca aaataaacat
     7741 gctcgacacc atcaaaatac acatagcaaa tcgtaacatt acagaaatcc aaaaacgact
     7801 acaaacgaga catacatttt taccagaata atcccagaaa aatctcagaa agatgagttc
     7861 tttgccgctc atctagcaca aatgagctag cgaacacagt caccagcgcc cttttatgcg
     7921 cacgaatcac acttccgggc tcgagcgttt gcgatccaaa aactactgca tacaactaaa
     7981 acgatttatt tctcccatga aacgacatat ctaagtcaga caacgaacac gatataaaaa
     8041 tctattaaac cgaaatgtgg cccgacatct aaaaataccg ttcggctttt ttactgtaac
     8101 gactttttac taacggttaa ccacatcctc atccaaaccg aaaaccttta cgtattgcgt
     8161 cagcgacaag ggcgtggact ttgccgtaaa atgcatataa aaaggcttgt cgcccgtcag
     8221 aagtattagg gatcaaactg taagtctcat acacggcaaa acagcgaatg ctaaaccgcg
     8281 aactacaaat cacgtgaagc ttgatatcga attcctgcag cccggggg
//
